The effect of environmental and therapeutic concentrations of antibiotics on nitrate reduction rates in river sediment
Abstract
The use of antibiotics in both human and veterinary medicine has led to increased presence of these compounds and antibiotic resistance in the environment. In this study, the effect of low, environmentally relevant (<mg L(-1)) and high therapeutic (>mg L(-1)) concentrations of vancomycin (VA), flumequine (FLU), and sulfamethoxazole (SMX) on nitrate reduction rates was studied in river sediments. Nitrate reduction rates were determined by supplying intact sediments for several weeks with both nitrate and antibiotics (ng L(-1), μg L(-1), and mg L(-1) concentrations), including a non-amended control. Furthermore the concentrations of the three investigated antibiotics were measured in the initial (natural) sediments and the sediments supplied with the antibiotics. The antibiotic concentrations in the sediments decreased (on average 62% for FLU and 93% for SMX) during the experiments, indicating loss of antibiotics due to sorption or (bio) degradation. Nitrate reduction rates were not affected by environmental concentrations of VA, FLU and SMX. FLU and SMX only partially inhibited nitrate reduction rates at high, therapeutic concentrations by 41 and 39% respectively. The three tested antibiotics significantly enhanced the production of nitrite, an intermediate in dissimilatory nitrate reduction. Nitrite production increased 1.9 and 1.4 fold for environmental VA concentrations (107 and 187 μg L(-1) respectively), application of 58 mg L(-1) SMX resulted in a 7.5 fold increase and augmented 16 and 8.5 fold in the presence of respectively 13 μg L(-1) and 52 mg L(-1) FLU. Even though inhibition of nitrate reduction rates was observed at therapeutic antibiotic concentrations, nitrate reduction proceeded under all experimental conditions, indicating the presence of resistance toward these antibiotics among the nitrate reducing bacteria. The accumulation of nitrite suggests that the nitrite reduction step was more affected than the overall nitrate reduction process.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Low levels of common antibiotics contaminate groundwater and soil and can disrupt bacterial denitrification.
“Recent ecological findings show that even low levels of common antibiotics contaminate groundwater and soil and can disrupt bacterial denitrification[424–426]”
From Enterosalivary nitrate metabolism and the microbiome: Intersection of microbial metabolism, nitric oxide and diet in cardiac and pulmonary vascular health (2017) · cites this paper as [425]
Cites
Environmental Chemistry of Soils
McBride, M. B. (1994) · Medical Entomology and Zoology · Cited by 5,871 · see citing works
Antibiotics in the aquatic environment – A review – Part I
Kümmerer, K. (2009) · Chemosphere · doi:10.1016/j.chemosphere.2008.11.086 · Cited by 4,485 · see citing works
Cell biology and molecular basis of denitrification
Zumft, W. G. (1997) · Microbiology and Molecular Biology Reviews · doi:10.1128/mmbr.61.4.533-616.1997 · Cited by 3,483 · see citing works
Occurrence, fate and effects of pharmaceutical substances in the environment- A review
Halling-Sørensen, B., Nors Nielsen, S., Lanzky, P. F., Ingerslev, F., Holten Lützhøft, H. C., & Jørgensen, S. E. (1998) · Chemosphere · doi:10.1016/s0045-6535(97)00354-8 · Cited by 3,285 · see citing works
Biology of anaerobic microorganisms
(1989) · Choice Reviews Online · doi:10.5860/choice.26-3877 · Cited by 2,252 · see citing works
Sorption of Veterinary Pharmaceuticals in Soils: A Review
Tolls, J. (2001) · Environmental Science & Technology · doi:10.1021/es0003021 · Cited by 1,390 · see citing works
(1988) · Medical Entomology and Zoology · Cited by 1,320 · see citing works
Pharmaceutical antibiotic compounds in soils – a review
Thiele‐Bruhn, S. (2003) · Journal of Plant Nutrition and Soil Science · doi:10.1002/jpln.200390023 · Cited by 1,313 · see citing works
The occurrence of antibiotics in an urban watershed: From wastewater to drinking water
Watkinson, A., Murby, E. J., Kolpin, D. W., & Costanzo, S. D. (2009) · The Science of The Total Environment · doi:10.1016/j.scitotenv.2008.11.059 · Cited by 1,200 · see citing works
Fatta-Kassinos, D., Meriç, S., & Nikolaou, A. (2011) · Analytical and Bioanalytical Chemistry · doi:10.1007/s00216-010-4300-9 · Cited by 885 · see citing works
Biology of anaerobic microorganisms
(1989) · Research in Microbiology · doi:10.1016/0923-2508(89)90030-2 · Cited by 861 · see citing works
Pharmaceuticals in groundwaters
Sacher, F., Lange, F. T., Brauch, H., & Blankenhorn, I. (2001) · Journal of Chromatography A · doi:10.1016/s0021-9673(01)01266-3 · Cited by 825 · see citing works
Pharmaceuticals in the Environment
Kümmerer, K. (2010) · Annual Review of Environment and Resources · doi:10.1146/annurev-environ-052809-161223 · Cited by 820 · see citing works
Significance of antibiotics in the environment
Kummerer, K. (2003) · Journal of Antimicrobial Chemotherapy · doi:10.1093/jac/dkg293 · Cited by 746 · see citing works
Presence of therapeutic drugs in the environment
Zuccato, E., Calamari, D., Natangelo, M., & Fanelli, R. (2000) · The Lancet · doi:10.1016/s0140-6736(00)02270-4 · Cited by 703 · see citing works
Ecosystem response to antibiotics entering the aquatic environment
Costanzo, S. D., Murby, J., & Bates, J. R. (2004) · Marine Pollution Bulletin · doi:10.1016/j.marpolbul.2004.10.038 · Cited by 593 · see citing works
Determination of Antibiotic Residues in Manure, Soil, and Surface Waters
Christian, T., Schneider, R. J., Färber, H. A., Skutlarek, D., Meyer, M. T., & Goldbach, H. E. (2003) · Acta hydrochimica et hydrobiologica · doi:10.1002/aheh.200390014 · Cited by 538 · see citing works
Occurrence and fate of antibiotics in the Seine River in various hydrological conditions
Tamtam, F., Mercier, F., Bot, B. L., Eurin, J., Dinh, Q. T., Clément, M., & Chevreuil, M. (2008) · The Science of The Total Environment · doi:10.1016/j.scitotenv.2007.12.009 · Cited by 499 · see citing works
Self-citationEffects of sulfonamide and tetracycline antibiotics on soil microbial activity and microbial biomass
Thiele‐Bruhn, S., & Beck, I. (2005) · Chemosphere · doi:10.1016/j.chemosphere.2005.01.023 · Cited by 487 · see citing works
Effect of antibiotics in the environment on microbial populations
Ding, C., & He, J. (2010) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-010-2649-5 · Cited by 467 · see citing works
Lindberg, R. H., Jarnheimer, P.-Å., Olsén, B., Johansson, M. I., & Tysklind, M. (2004) · Chemosphere · doi:10.1016/j.chemosphere.2004.09.015 · Cited by 455 · see citing works
Occurrence and Environmental Fate of Veterinary Antibiotics in the Terrestrial Environment
Kim, K.-R., Owens, G., Kwon, S.-I., So, K.-H., Lee, D.-B., & Ok, Y. S. (2010) · Water Air & Soil Pollution · doi:10.1007/s11270-010-0412-2 · Cited by 452 · see citing works
Fluoroquinolones in soil—risks and challenges
Picó, Y., & Andreu, V. (2006) · Analytical and Bioanalytical Chemistry · doi:10.1007/s00216-006-0843-1 · Cited by 355 · see citing works
Persistence of antibacterial agents in marine sediments
Hektoen, H., Berge, J. A., Hormazábal, V., & Yndestad, M. (1995) · Aquaculture · doi:10.1016/0044-8486(94)00310-k · Cited by 351 · see citing works
Visible-Light-Mediated TiO2 Photocatalysis of Fluoroquinolone Antibacterial Agents
Paul, T., Miller, P. L., & Strathmann, T. J. (2007) · Environmental Science & Technology · doi:10.1021/es070097q · Cited by 347 · see citing works
Lalumera, G. M., Calamari, D., Galli, P., Castiglioni, S., Crosa, G. A., & Fanelli, R. (2003) · Chemosphere · doi:10.1016/j.chemosphere.2003.08.001 · Cited by 312 · see citing works
Occurrence of sulfonamide antimicrobials in private water wells in Washington County, Idaho, USA
Batt, A. L., Snow, D. D., & Aga, D. S. (2006) · Chemosphere · doi:10.1016/j.chemosphere.2006.01.029 · Cited by 298 · see citing works
Fate of the Antibiotic Sulfamethoxazole and Its Two Major Human Metabolites in a Water Sediment Test
Radke, M., Lauwigi, C., Heinkele, G., Mürdter, T. E., & Letzel, M. (2009) · Environmental Science & Technology · doi:10.1021/es900300u · Cited by 230 · see citing works
Dinh, Q. T., Alliot, F., Moreau-Guigon, E., Eurin, J., Chevreuil, M., & Labadie, P. (2011) · Talanta · doi:10.1016/j.talanta.2011.05.013 · Cited by 229 · see citing works
Self-citationEffects of the Antimicrobial Sulfamethoxazole on Groundwater Bacterial Enrichment
Underwood, J. C., Harvey, R. W., Metge, D. W., Repert, D. A., Baumgartner, L. K., Smith, R. L., Roane, T. M., & Barber, L. B. (2011) · Environmental Science & Technology · doi:10.1021/es103605e · Cited by 217 · see citing works
Ingerslev, F., Toräng, L., Loke, M.-L., et al. (2001) · Chemosphere · doi:10.1016/s0045-6535(00)00479-3 · Cited by 190 · see citing works
Mohatt, J. L., Hu, L., Finneran, K. T., & Strathmann, T. J. (2011) · Environmental Science & Technology · doi:10.1021/es200413g · Cited by 160 · see citing works
Ingerslev, F., & Halling‐Sørensen, B. (2001) · Ecotoxicology and Environmental Safety · doi:10.1006/eesa.2000.2026 · Cited by 159 · see citing works
Zielezny, Y., Groeneweg, J., Vereecken, H., et al. (2006) · Soil Biology and Biochemistry · doi:10.1016/j.soilbio.2006.01.031 · Cited by 155 · see citing works
Mechanisms of resistance to antibiotics
Wright, G. D. (2003) · Current Opinion in Chemical Biology · doi:10.1016/j.cbpa.2003.08.004 · Cited by 138 · see citing works
Potential rates and pathways of microbial nitrate reduction in coastal sediments
Laverman, A. M., Cappellen, P. V., van Rotterdam-Los, D., et al. (2006) · FEMS Microbiology Ecology · doi:10.1111/j.1574-6941.2006.00155.x · Cited by 98 · see citing works
Self-citationDegradation of oxolinic acid and flumequine in aquaculture pond waters and sediments
Lai, H., & Lin, J.-J. (2009) · Chemosphere · doi:10.1016/j.chemosphere.2008.12.060 · Cited by 93 · see citing works
Aerobic degradation and photolysis of tylosin in water and soil
Hu, D., & Coats, J. R. (2007) · Environmental Toxicology and Chemistry · doi:10.1897/06-197r.1 · Cited by 87 · see citing works
Degradation of the fluoroquinolone enrofloxacin by wood-rotting fungi
Martens, R., Wetzstein, H.-G., Zadražil, F., Capelari, M., Hoffmann, P., & Schmeer, N. (1996) · Applied and Environmental Microbiology · doi:10.1128/aem.62.11.4206-4209.1996 · Cited by 84 · see citing works
Liu, F., Ying, G., Yang, J., Zhou, L., Tao, R., Wang, L., Zhang, L., & Peng, P. (2010) · Environmental Chemistry · doi:10.1071/en09160 · Cited by 80 · see citing works
Pallud, C. E., Meile, C. D., Laverman, A. M., et al. (2006) · Marine Chemistry · doi:10.1016/j.marchem.2006.12.011 · Cited by 76 · see citing works
Self-citationMaki, T., Hasegawa, H., KITAMI, H., FUMOTO, K., Munekage, Y., & Ueda, K. (2006) · Fisheries Science · doi:10.1111/j.1444-2906.2006.01222.x · Cited by 73 · see citing works
Microbial models of soil metabolism: biotransformations of danofloxacin
Chen, Y., Rosazza, J. P. N., Reese, C., et al. (1997) · Journal of Industrial Microbiology & Biotechnology · doi:10.1038/sj.jim.2900409 · Cited by 66 · see citing works
Significance of antibiotics in the environment
Kummerer, K. (2003) · Journal of Antimicrobial Chemotherapy · doi:10.1093/jac/dkg386 · Cited by 58 · see citing works
Chloramphenicol Inhibition of Denitrifying Enzyme Activity in Two Agricultural Soils
Murray, R. E., & Knowles, R. (1999) · Applied and Environmental Microbiology · doi:10.1128/aem.65.8.3487-3492.1999 · Cited by 57 · see citing works
Viola, G., Facciolo, L., Canton, M., et al. (2004) · Chemistry & Biodiversity · doi:10.1002/cbdv.200490061 · Cited by 54 · see citing works
Primary Photophysical Properties of Moxifloxacin— A Fluoroquinolone Antibiotic
Lorenzo, F., Navaratnam, S., Edge, R., et al. (2008) · Photochemistry and Photobiology · doi:10.1111/j.1751-1097.2007.00269.x · Cited by 48 · see citing works
A 50-year record of quinolone and sulphonamide antimicrobial agents in Seine River sediments
Tamtam, F., Bot, B. L., Dinh, Q. T., et al. (2011) · Journal of Soils and Sediments · doi:10.1007/s11368-011-0364-1 · Cited by 47 · see citing works
Self-citationWerner, J. J., Chintapalli, M., Lundeen, R. A., et al. (2007) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf070101h · Cited by 38 · see citing works
Ilhan, Z. E., Ong, S. K., & Moorman, T. B. (2011) · Journal of Environmental Quality · doi:10.2134/jeq2011.0082 · Cited by 33 · see citing works
Is site preference of N2O a tool to identify benthic denitrifier N2O?
Mothet, A., Sébilo, M., Laverman, A. M., et al. (2013) · Environmental Chemistry · doi:10.1071/en13021 · Cited by 15 · see citing works
Self-citation
Flagged first, then by citations
Cited by
Sorted by citations
Koch, C. D., Gladwin, M. T., Freeman, B. A., Lundberg, J. O., Weitzberg, E., & Morris, A. (2017) · Free Radical Biology and Medicine · doi:10.1016/j.freeradbiomed.2016.12.015 · Cited by 186 · see citing works
- Authors
- Yan, C., Dinh, Q. T., Chevreuil, M., Garnier, J., Roose-Amsaleg, C., Labadie, P., & Laverman, A. M.
- Affiliations
- Milieux environnementaux, transferts et interactions dans les hydrosystèmes et les sols (FR)
- École Pratique des Hautes Études (FR)
- Centre National de la Recherche Scientifique (FR)
- Corresponding author
- Laverman, Anniet M.
- Venue
- Water Research
- Publisher
- Elsevier BV
- Published
- 2013-07
- Volume
- 47
- Issue
- 11
- Pages
- 3654-3662
- ISSN
- 0043-1354 (ISSN-L)
- OpenAlex
- W2072596475
- Cited by
- 67
- References
- 51
- Citations by year
- 2026: 2 · 2025: 3 · 2024: 3 · 2023: 5 · 2022: 7 · 2021: 2 · 2020: 4 · 2019: 10 · 2018: 6 · 2017: 5 · 2016: 12 · 2015: 5 · 2014: 1 · 2013: 2
- FWCI
- 5.20
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Water Research (publishedVersion)
- PubMed (publishedVersion)
- HAL (submittedVersion)
- Topics
- Physical Sciences › Environmental Science › Pollution › Pharmaceutical and Antibiotic Environmental Impacts
- Physical Sciences › Environmental Science › Environmental Chemistry › Environmental Chemistry and Analysis
- Physical Sciences › Environmental Science › Pollution › Wastewater Treatment and Nitrogen Removal
- Keywords
- river sediments, vancomycin, flumequine, sulfamethoxazole, nitrite production, dissimilatory nitrate reduction, antibiotic resistance, antibiotic sorption, antibiotic degradation, nitrate-reducing bacteria
- MeSH terms
- Anti-Bacterial Agents, Biodegradation, Environmental, France, Nitrates, Nitrites, Oxidation-Reduction, Sulfamethoxazole, Vancomycin, Water Pollutants, Chemical, Geologic Sediments, Fluoroquinolones, Rivers
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
