The DOI matches its Crossref record; 30 of the 30 references it cites were found in a registry or an index.
Chemical characterization methods for the analysis of structural extracellular polymeric substances (EPS)
Open accessAbstract
Biofilm structure and functionality depend on extracellular polymeric substances (EPS), but analytical methods for EPS often lack specificity which limits progress of biofilm research. EPS were extracted from aerobic granular sludge and analyzed with frequently applied colorimetric methods. The colorimetric methods were evaluated based on their applicability for EPS analysis. EPS fractions of interest were proteins, sugars, uronic acids and phenolic compounds. The applied methods (Lowry method, bicinchoninic acid assay, phenol sulfuric acid method, carbazole sulfuric acid method) were investigated in terms of their sensitivity towards the selected standard compound. Interference of compounds present in EPS with the colorimetric methods was further evaluated. All methods showed to be highly depending on the choice of standard compound and susceptible towards interference by compounds present in EPS. This study shows that currently used colorimetric methods are not capable of accurately characterizing EPS. More advanced methods are needed to be able to draw conclusions about biofilm composition, structure and functionality.
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PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENT
Lowry, O., Rosebrough, N., Farr, A. L., & Randall, R. (1951) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)52451-6 · Cited by 317,331
Standard methods: For the examination of water and waste water
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Colorimetric Method for Determination of Sugars and Related Substances
DuBois, M., Gilles, K. A., Hamilton, J. K., Rebers, P. A., & Smith, F. (1956) · Analytical Chemistry · doi:10.1021/ac60111a017 · Cited by 52,269
Measurement of protein using bicinchoninic acid
Smith, P. K., Krohn, R. I., Hermanson, G. T., Mallia, A. K., Gartner, F. H., Provenzano, M. D., Fujimoto, E. K., Goeke, N. M., Olson, B. J., & Klenk, D. C. (1985) · Analytical Biochemistry · doi:10.1016/0003-2697(85)90442-7 · Cited by 18,482
Standard methods for the examination of water and wastewater
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Flemming, H.-C., & Wingender, J. (2010) · Nature Reviews Microbiology · doi:10.1038/nrmicro2415 · Cited by 10,137
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ON TYROSINE AND TRYPTOPHANE DETERMINATIONS IN PROTEINS
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Extraction of extracellular polymers from activated sludge using a cation exchange resin
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A NEW SPECIFIC COLOR REACTION OF HEXURONIC ACIDS
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Cited by
Vandana, & Das, S. (2022) · Carbohydrate Polymers · doi:10.1016/j.carbpol.2022.119536 · Cited by 199
Cheah, Y. T., & Chan, D. J. C. (2022) · Journal of Applied Microbiology · doi:10.1111/jam.15455 · Cited by 108
Metadata
- Authors
- Felz, S., Vermeulen, P., van Loosdrecht, M. C. M., & Lin, Y. M.
- Venue
- Water Research
- Publisher
- Elsevier BV
- Published
- 2019-06
- Volume
- 157
- Pages
- 201-208
- ISSN
- 0043-1354 (ISSN-L)
- OpenAlex
- W2925995880
- Cited by
- 365
- References
- 30
- FWCI
- 8.14
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Felz, S., Vermeulen, P., van Loosdrecht, M. C. M., & Lin, Y. M. (2019). Chemical characterization methods for the analysis of structural extracellular polymeric substances (EPS). Water Research. https://doi.org/10.1016/j.watres.2019.03.068
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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