CRISPR–Cas12a base editors confer efficient multiplexed genome editing in rice
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Delivery of the enzymes expressed limitations of less efficiency, tissue-specificity, immunogenicity, and species-specificity.
“The delivery of the enzymes expressed the limitations of less efficiency, tissue-specific, immunogenicity, and species-specificity, etc. (Chen et al., 2020; Cheng et al., 2023).”
From Emerging applications of gene editing technologies for the development of climate-resilient crops (2025) · cites this paper as Cheng et al., 2023
The main text (page unavailable) discusses editing efficiency at different rice target sites, not the efficiency of enzyme delivery. Searches for delivery limitations, tissue specificity, immunogenicity, and species specificity found no passage addressing those claims. (LLM, conf=0.72)
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Richter, M. F., Zhao, K. T., Eton, E., Lapinaite, A., Newby, G. A., Thuronyi, B. W., Wilson, C., Koblan, L. W., Zeng, J., Bauer, D. E., et al. (2020) · Nature Biotechnology · doi:10.1038/s41587-020-0453-z · Cited by 1,020 · see citing works
Kleinstiver, B. P., Sousa, A. A., Walton, R. T., Tak, Y. E., Hsu, J. Y., Clement, K., Welch, M. M., Horng, J. E., Malagon-Lopez, J., Scarfò, I., et al. (2019) · Nature Biotechnology · doi:10.1038/s41587-018-0011-0 · Cited by 680 · see citing works
A CRISPR–Cpf1 system for efficient genome editing and transcriptional repression in plants
Tang, X. D., Lowder, L. G., Zhang, T., Malzahn, A. A., Zheng, X., Voytas, D. F., Zhong, Z., Chen, Y., Ren, Q., Li, Q., et al. (2017) · Nature Plants · doi:10.1038/nplants.2017.18 · Cited by 619 · see citing works
Self-citationBase editing with a Cpf1–cytidine deaminase fusion
Li, X., Wang, Y., Liu, Y., Yang, B., Wang, X., Wei, J., Lu, Z., Zhang, Y., Wu, J., Huang, X., et al. (2018) · Nature Biotechnology · doi:10.1038/nbt.4102 · Cited by 426 · see citing works
Self-citationThe emerging and uncultivated potential of CRISPR technology in plant science
Zhang, Y., Malzahn, A. A., Sretenovic, S., & Qi, Y. (2019) · Nature Plants · doi:10.1038/s41477-019-0461-5 · Cited by 404 · see citing works
Self-citationPrecise plant genome editing using base editors and prime editors
Molla, K. A., Sretenovic, S., Bansal, K. C., & Qi, Y. (2021) · Nature Plants · doi:10.1038/s41477-021-00991-1 · Cited by 387 · see citing works
Self-citationEfficient targeted mutagenesis of rice and tobacco genomes using Cpf1 from Francisella novicida
Endo, A., Mikami, M., Kaya, H., & Toki, S. (2016) · Scientific Reports · doi:10.1038/srep38169 · Cited by 297 · see citing works
Engineering CRISPR/LbCas12a for highly efficient, temperature‐tolerant plant gene editing
Schindele, P., & Puchta, H. (2019) · Plant Biotechnology Journal · doi:10.1111/pbi.13275 · Cited by 213 · see citing works
Zhang, Y., Ren, Q., Tang, X., Liu, S., Malzahn, A. A., Zhou, J., Wang, J., Yin, D., Pan, C., Yuan, M., et al. (2021) · Nature Communications · doi:10.1038/s41467-021-22330-w · Cited by 202 · see citing works
Self-citationImproved plant cytosine base editors with high editing activity, purity, and specificity
Ren, Q., Sretenovic, S., Liu, G., Zhong, Z., Wang, J., Huang, L., Tang, X., Guo, Y., Liu, L., Wu, Y., et al. (2021) · Plant Biotechnology Journal · doi:10.1111/pbi.13635 · Cited by 131 · see citing works
Self-citationCas12a Base Editors Induce Efficient and Specific Editing with Low DNA Damage Response
Wang, X., Ding, C., Yu, W., Wang, Y., He, S., Yang, B., Xiong, Y., Wei, J., Li, J., Liang, J., et al. (2020) · Cell Reports · doi:10.1016/j.celrep.2020.107723 · Cited by 110 · see citing works
Self-citationHighly efficient C‐to‐T and A‐to‐G base editing in a Populus hybrid
Li, G., Sretenovic, S., Eisenstein, E., Coleman, G. D., & Qi, Y. (2021) · Plant Biotechnology Journal · doi:10.1111/pbi.13581 · Cited by 60 · see citing works
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Targeted genome-modification tools and their advanced applications in crop breeding
Li, B., Sun, C., Li, J., & Gao, C. (2024) · Nature Reviews Genetics · doi:10.1038/s41576-024-00720-2 · Cited by 259 · see citing works
Self-citationCRISPR–Cas applications in agriculture and plant research
Tuncel, A., Pan, C., Clem, J. S., Liu, D., & Qi, Y. (2025) · Nature Reviews Molecular Cell Biology · doi:10.1038/s41580-025-00834-3 · Cited by 139 · see citing works
Self-citationFan, T., Cheng, Y., Wu, Y., Liu, S., Tang, X. D., He, Y., Liao, S., Zheng, X., Zhang, T., Qi, Y., et al. (2024) · Nature Communications · doi:10.1038/s41467-024-49473-w · Cited by 56 · see citing works
Self-citationPrecise fine-turning of GhTFL1 by base editing tools defines ideal cotton plant architecture
Wang, G., Wang, F., Xu, Z., Wang, Y., Zhang, C., Zhou, Y., Hui, F., Yang, X., Nie, X., Zhang, X., et al. (2024) · Genome biology · doi:10.1186/s13059-024-03189-8 · Cited by 46 · see citing works
Emerging applications of gene editing technologies for the development of climate-resilient crops
Chavhan, R. L., Jaybhaye, S. G., Hinge, V. R., Deshmukh, A. S., Shaikh, U. S., Jadhav, P. K., Kadam, U. S., & Hong, J. C. (2025) · Frontiers in Genome Editing · doi:10.3389/fgeed.2025.1524767 · Cited by 43 · see citing works
The type V effectors for CRISPR/Cas-mediated genome engineering in plants
Zhang, R., Chai, N., Liu, T., Zheng, Z., Lin, Q., Xie, X., Wen, J., Yang, Z. F., Liu, Y., & Zhu, Q. (2024) · Biotechnology Advances · doi:10.1016/j.biotechadv.2024.108382 · Cited by 36 · see citing works
CRISPR/Cas genome editing in soybean: challenges and new insights to overcome existing bottlenecks
Alves, N. S. F., Pinto, C. E. M., Távora, F. T. P. K., de Melo, B. P., Arraes, F. B. M., Lourenço‐Tessutti, I. T., de Moura, S. M., de Oliveira, A. C., Morgante, C. V., Qi, Y., et al. (2024) · Journal of Advanced Research · doi:10.1016/j.jare.2024.08.024 · Cited by 35 · see citing works
Self-citationDirected evolution rice genes with randomly multiplexed sgRNAs assembly of base editors
Zhang, A., Shan, T., Sun, Y., Chen, Z., Hu, J., Hu, Z., Ming, Z., Zhu, Z., Li, X., He, J., et al. (2023) · Plant Biotechnology Journal · doi:10.1111/pbi.14156 · Cited by 32 · see citing works
Multiplexed in-situ mutagenesis driven by a dCas12a-based dual-function base editor
Wu, Y., Li, Y., Liu, Y., Xiu, X., Liu, J., Zhang, L., Li, J., Du, G., Lv, X., Chen, J., et al. (2024) · Nucleic Acids Research · doi:10.1093/nar/gkae228 · Cited by 29 · see citing works
Self-citationHe, Y., Han, Y., Ma, Y., Liu, S., Fan, T., Liang, Y., Tang, X., Zheng, X., Wu, Y., Zhang, T., et al. (2024) · Plant Biotechnology Journal · doi:10.1111/pbi.14363 · Cited by 29 · see citing works
Self-citationGene editing of non-coding regulatory DNA and its application in crop improvement
Chen, Y.-H., Lu, J., Xia, Y., Huang, L., Zhang, C., Liu, Q., & Li, Q. (2023) · Journal of Experimental Botany · doi:10.1093/jxb/erad313 · Cited by 19 · see citing works
Advances and Applications of Plant Base Editing Technologies
Peng, H. F., Li, J., Sun, K., Tang, H., Huang, W., Li, X., Wang, S., Ding, K., Han, Z., Li, Z., et al. (2025) · International Journal of Molecular Sciences · doi:10.3390/ijms26199452 · Cited by 17 · see citing works
Self-citationDeveloping a CRISPR/FrCas9 system for core promoter editing in rice
Wang, H., Ding, J., Zhu, J., Liu, X., Xu, R., Qin, R., Gu, D., Li, M., Wei, P., & Li, J. (2024) · aBIOTECH · doi:10.1007/s42994-024-00157-5 · Cited by 10 · see citing works
Self-citationCRISPR–Cas systems and applications for crop bioengineering
Uranga, M., Martín‐Hernández, A. M., Storme, N. D., & Pasin, F. (2024) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2024.1483857 · Cited by 11 · see citing works
Gene editing tool kit in millets: present status and future directions
Sapara, V., Khisti, M., Yogendra, K. N., & Reddy, P. S. (2024) · The Nucleus · doi:10.1007/s13237-024-00485-3 · Cited by 8 · see citing works
A comprehensive all-in-one CRISPR toolbox for large-scale screens in plants
Cheng, Y., Li, G., Qi, A., Mandlik, R., Pan, C., Wang, D., Ge, S., & Qi, Y. (2025) · The Plant Cell · doi:10.1093/plcell/koaf081 · Cited by 9 · see citing works
Self-citationOptimizing ErCas12a for efficient gene editing in Arabidopsis thaliana
Pietralla, J., Capdeville, N., Schindele, P., & Puchta, H. (2023) · Plant Biotechnology Journal · doi:10.1111/pbi.14194 · Cited by 8 · see citing works
Mapping the plant genome: CRISPR/Cas9 as a toolkit for functional genomics
Thabet, S. G., Elkelish, A., Dawood, M. F. A., Safhi, F. A., Alhudhaibi, A. M., Börner, A., & Alqudah, A. M. (2025) · Acta Physiologiae Plantarum · doi:10.1007/s11738-025-03872-8 · Cited by 4 · see citing works
Zhang, C., Li, J., Li, Y., Yan, L., Yong, C. S. Y., Li, S., He, Y., & Xia, L. (2026) · Journal of Integrative Plant Biology · doi:10.1111/jipb.70154 · Cited by 4 · see citing works
Self-citationLiu, X., Jin, S., Xiao, Z., Ma, C., Wang, Q., Wang, H., Zhou, R., Gu, D., Xu, R., Qin, R., et al. (2026) · Journal of Integrative Plant Biology · doi:10.1111/jipb.70177 · Cited by 4 · see citing works
Self-citationGenome editing in rice: toward climate-resilient, nutrient-rich yields
Huang, X., He, W., Bassié, L., Christou, P., & Capell, T. (2026) · Trends in Plant Science · doi:10.1016/j.tplants.2026.02.007 · Cited by 3 · see citing works
Single‐Cell CRISPR: An Efficient Strategy for Decoding Plant Cis ‐Regulatory Complexity
Zhang, Y., Luo, X., Li, H., Jin, S., Zhang, X., & Wang, Y. (2026) · Plant Biotechnology Journal · doi:10.1111/pbi.70647 · Cited by 3 · see citing works
Self-citationEnhancing CRISPR‐Cas12a base editing in plants with LbCas12a variants and introns
Cheng, Y., Li, G., Zhou, M., Mandlik, R., Wang, D., & Qi, Y. (2026) · Journal of Integrative Plant Biology · doi:10.1111/jipb.70249 · Cited by 3 · see citing works
Self-citationBase editing in rice using nuclease‐deactivated CRISPR/Cas‐SF01
Jia, M., Xie, Y., Wei, C., Wang, H., Xue, L., Zou, C., Zhu, J., & Wang, M. (2026) · Journal of Integrative Plant Biology · doi:10.1111/jipb.70265 · Cited by 3 · see citing works
From Shared Mechanisms to Precision Breeding: Engineering Cold and Drought Cross-Tolerance in Crops
Yang, X., Jia, Z., Liu, Y., Wang, X., Chen, J., Liu, Y., & Chen, M.-X. (2026) · International Journal of Molecular Sciences · doi:10.3390/ijms27052497 · Cited by 2 · see citing works
Research progress of CRISPR/Cas gene editing technology in rice breeding
Li, K., Wu, C., & Sui, Y. (2023) · Chinese Science Bulletin (Chinese Version) · doi:10.1360/tb-2023-1134 · Cited by 2 · see citing works
High-Efficiency Cas12a-Mediated A-to-G Base Editing in Rice
Cheng, Y., & Qi, Y. (2025) · Springer protocols handbooks/Springer protocols · doi:10.1007/978-1-0716-4358-7_10 · Cited by 1 · see citing works
Self-citationAdvances in multiplex precision genome editing in eukaryotic and prokaryotic systems
Golla, D. A., Sun, C., Haugh, L., Straub, N., & Gao, X. (2026) · Current Opinion in Biotechnology · doi:10.1016/j.copbio.2026.103470 · Cited by 1 · see citing works
Establishment of an efficient and PAM ‐relaxed LbCas12a genome editing tool in plants
Wang, Y., Xu, H., Lu, W., Wan, Z., Xu, Z., Wang, W., Chen, C., Pan, E., Jiang, F., Liu, T., et al. (2026) · New Phytologist · doi:10.1111/nph.71498 · Cited by 1 · see citing works
Precision genetic manipulation: CRISPR toolkits reshape plant biology
Li, Z., Xiong, X., Cao, R., & Li, J. (2026) · PLANT PHYSIOLOGY · doi:10.1093/plphys/kiag696 · Cited by 1 · see citing works
Self-citationZhong, Z., & Qin, P. (2025) · Chinese Science Bulletin (Chinese Version) · doi:10.1360/tb-2024-0697 · Cited by 1 · see citing works
Targeted Base Editing in Rice Using Target-AID
Nguyen, S. M., Taoka, K.-I., & Nishida, K. (2025) · Springer protocols handbooks/Springer protocols · doi:10.1007/978-1-0716-4358-7_1
Potentials and Challenges of CRISPR/Cas9 Technique for Salinity Stress Management
Saha, A., Banerjee, A., Mandal, K., & Upadhyaya, G. (2026) · Springer eBooks · doi:10.1007/978-3-032-24001-9_11
Janthabut, T., Pongjaroenkit, S., Khemkladngoen, N., Lithanatudom, S. K., Chanchay, P., Vuttipongchaikij, S., & Sakulsingharoj, C. (2026) · Transgenic Research · doi:10.1007/s11248-026-00511-x
Wu, X., Liu, M., Yan, F., Ma, D. M., Ren, B., & Zhou, H. (2026) · PLANT PHYSIOLOGY · doi:10.1093/plphys/kiag732
From base transitions to expanded editing tools: development and applications of plant base editors
Sui, N., Zhang, J., Xu, W., 庞美新, Zhang, R., Chai, N., Wen, J., Liu, T., Zheng, Z., Huang, J., et al. (2026) · Biotechnology Advances · doi:10.1016/j.biotechadv.2026.109074
- Authors
- Cheng, Y., Zhang, Y., Li, G., Fang, H., Sretenovic, S., Fan, A., Li, J., Xu, J., Que, Q., & Qi, Y.
- Affiliations
- University of Maryland, College Park (US)
- Syngenta (United States) (US)
- Syngenta (China) (CN)
- Institute for Bioscience and Biotechnology Research (US)
- Corresponding author
- Qi, Yiping
- Venue
- Plant Communications
- Publisher
- Elsevier BV
- Published
- 2023-07
- Volume
- 4
- Issue
- 4
- Pages
- 100601
- ISSN
- 2590-3462 (ISSN-L)
- PMC
- PMC10363544
- OpenAlex
- W4365517431
- Cited by
- 36
- References
- 13
- Citations by year
- 2026: 14 · 2025: 8 · 2024: 10 · 2023: 4
- FWCI
- 3.21
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Plant Communications (publishedVersion, cc-by-nc-nd)
- PubMed Central (publishedVersion, cc-by-nc-nd)
- PubMed (publishedVersion)
- Funders
- Syngenta International
- National Science Foundation (IOS-2029889, IOS-2132693)
- U.S. Department of Agriculture
- NSF
- USDA NIFA (2021-67013-34554)
- Foundation for Food and Agriculture Research (593603)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › CRISPR and Genetic Engineering
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Molecular Biology Research
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Genetic and Mutation Studies
- Keywords
- CRISPR/Cas12a, base editors, multiplexed genome editing, rice, genome editing efficiency
- MeSH terms
- Gene Editing, Oryza, CRISPR-Cas Systems
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