FoxA transcription factors are essential for the development of dorsal axial structures
Open accessAbstract
In vertebrates, embryonic structures present at the dorsal midline, prechordal plate, notochord, hypochord and floor plate share a common embryonic origin. In zebrafish, they derive from a pool of progenitors located within the embryonic shield at the onset of gastrulation. The molecular mechanisms responsible for the common development of these structures remain unknown. Based on their spatial and temporal expression, transcription factors of the Forkhead box A (FoxA) family appeared to be good candidates to play such a role. In agreement with this hypothesis, we found that simultaneous knockdown of FoxA2 and FoxA3 abolish the formation of all axial derivatives, while overexpression of these transcription factors strongly enlarges dorsal mesodermal territories. We establish that, in FoxA2-FoxA3 double morphants, precursors of axial tissues are correctly induced at early gastrula stage, but their dorsal midline identity is not maintained during development and we found that progenitors of these tissues are cell-autonomously re-specified to form muscle fibers as well as cells of the ventral neural tube. Our study provides the first example of a specific loss of all dorsal midline tissues and demonstrates that members of the FoxA family have redundant functions essential to maintain the axial identity of prechordal plate, notochord, floor plate and hypochord progenitors during gastrulation.
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Some known notochord regulators, such as foxa3, were not identified as they are filtered out in the first steps of data processing owing to low expression.
“However, it is important to note that some known notochord regulators, such as foxa3 (ref. 41), were not identified as they are filtered out in the first steps of data processing owing to low expression.”
From Dissecting cell identity via network inference and in silico gene perturbation (2023) · cites this paper as [41]
A perturbation cannot be simulated if a TF-binding motif is unknown or TF expression is too sparse, as in the case of foxa3 in zebrafish.
“For example, a perturbation cannot be simulated if a TF-binding motif is unknown or TF expression is too sparse, as we note in the case of foxa3 in zebrafish41.”
From Dissecting cell identity via network inference and in silico gene perturbation (2023) · cites this paper as [41]
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Dissecting cell identity via network inference and in silico gene perturbation
Kamimoto, K., Stringa, B., Hoffmann, C. M., Jindal, K., Solnica-Krezel, L., & Morris, S. A. (2023) · Nature · doi:10.1038/s41586-022-05688-9 · Cited by 677 · see citing works
- Authors
- Dal-Pra, S., Thisse, C., & Thisse, B.
- Affiliations
- University of Virginia (US)
- Corresponding author
- Thisse, Bernard
- Venue
- Developmental Biology
- Publisher
- Elsevier BV
- Published
- 2011-02
- Volume
- 350
- Issue
- 2
- Pages
- 484-495
- ISSN
- 0012-1606 (ISSN-L)
- OpenAlex
- W1992919344
- Cited by
- 35
- References
- 63
- Citations by year
- 2026: 2 · 2024: 2 · 2023: 6 · 2022: 6 · 2021: 3 · 2020: 4 · 2019: 1 · 2018: 2 · 2017: 1 · 2016: 2 · 2014: 2 · 2013: 3 · 2012: 1
- FWCI
- 0.52
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Developmental Biology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Institut National de la Santé et de la Recherche Médicale
- Center National de la Recherche Scientifique
- Ministère de la Recherche
- Hôpital Universitaire de Strasbourg
- Association pour la Recherche sur le Cancer
- Ligue Nationale Contre le Cancer
- University of Virginia
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Wnt/β-catenin signaling in development and cancer
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Zebrafish Biomedical Research Applications
- Keywords
- FoxA transcription factor, FOXA2, FOXA3, prechordal plate, notochord, floor plate, embryonic shield, gastrulation, dorsal mesoderm, muscle fiber development, ventral neural tube, zebrafish
- MeSH terms
- Animals, Mesoderm, Zebrafish, Zebrafish Proteins, Hepatocyte Nuclear Factor 3-gamma, Forkhead Transcription Factors, Gastrulation
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