The DOI matches its Crossref record; 49 of the 51 references it cites were found in a registry or an index.
C2C12 myoblasts release micro-vesicles containing mtDNA and proteins involved in signal transduction
Abstract
Micro-vesicles can be released by different cell types and operate as 'safe containers' mediating inter-cellular communication. In this work we investigated whether cultured myoblasts could release exosomes. The reported data demonstrate, for the first time, that C2C12 myoblasts release micro-vesicles as shown by the presence of two exosome markers (Tsg101 and Alix proteins). Using real-time PCR analysis it was shown that these micro-vesicles, like other cell types, carry mtDNA. Proteomic characterization of the released micro-vesicle contents showed the presence of many proteins involved in signal transduction. The bioinformatics assessment of the Disorder Index and Aggregation Index of these proteins suggested that C2C12 micro-vesicles mainly deliver the machinery for signal transduction to target cells rather than key proteins involved in hub functions in molecular networks. The presence of IGFBP-5 in the purified micro-vesicles represents an exception, since this binding protein can play a key role in the modulation of the IGF-1 signalling pathway. In conclusion, the present findings demonstrate that skeletal muscle cells release micro-vesicles, which probably have an important role in the communication processes within skeletal muscles and between skeletal muscles and other organs. In particular, the present findings suggest possible new diagnostic approaches to skeletal muscle diseases.
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“Interestingly, mitochondrial DNA (Guescini et al., 2010), histones, and nuclear proteins were also found in ELV-MB (Guescini et al., 2010; Forterre et al., 2014a; Sork et al., 2018) and ELV-MT (Le Bihan et al., 2012; Forterre et al., 2014a).”
From Skeletal Muscle-Released Extracellular Vesicles: State of the Art (2019) · cites this paper as Guescini et al., 2010
In the citing paper
“It has been described that myoblasts produce small ELVs (ELV-MB) (Guescini et al., 2010; Forterre et al., 2014a,b; Sork et al., 2018) likely reflecting the intense macromolecule/RNA/lipids turn-over that occurs during cell proliferation (Johnstone et al., 1991; Desdin-Mico and Mittelbrunn, 2017).”
From Skeletal Muscle-Released Extracellular Vesicles: State of the Art (2019) · cites this paper as Guescini et al., 2010
In the citing paper
“During the last decade, it has been shown that muscle cells also release extracellular vesicles (EVs) into the extracellular milieu, which represent new paracrine and endocrine signals that have modified our conceptual basis to explain how muscles communicate to other organs (Guescini et al., 2010; Le Bihan et al., 2012; Romancino et al., 2013; Forterre et al., 2014a).”
From Skeletal Muscle-Released Extracellular Vesicles: State of the Art (2019) · cites this paper as Guescini et al., 2010
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THIS ARTICLE HAS BEEN RETRACTED Exercise induces interleukin‐8 expression in human skeletal muscle
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“Our study provides evidence that exosomes can carry large fragments ( 10 kb) of double-stranded genomic DNA, apart from mitochondrial DNA and small amounts of singlestranded DNA (13, 14).”
BackgroundInfluentialExosomes as mediators of intercellular crosstalk in metabolism
Isaac, R., Reis, F. C. G., Ying, W., & Olefsky, J. M. (2021) · Cell Metabolism · doi:10.1016/j.cmet.2021.08.006 · Cited by 860
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“Although the mitochondrialND1 gene was previously identified in the EVs of glioblastoma cell lines and astrocytes (11), the presence of mitochondrial DNA has not been examined in EVs from patients with breast cancer.”
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“has been observed in several normal and pathological conditions (35, 36), and it will now be important to test whether this is linked to local”
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Metadata
- Authors
- Guescini, M., Guidolin, D., Vallorani, L., Casadei, L., Gioacchini, A. M., Tibollo, P., Battistelli, M., Falcieri, E., Battistin, L., Agnati, L. F., & Stocchi, V.
- Affiliations
- University of Urbino (IT)
- University of Padua (IT)
- IRCCS San Camillo Hospital (IT)
- Corresponding author
- Guescini, M.
- Stocchi, V.
- ORCID
- Venue
- Experimental Cell Research
- Publisher
- Elsevier BV
- Published
- 2010-07
- Volume
- 316
- Issue
- 12
- Pages
- 1977-1984
- ISSN
- 0014-4827 (ISSN-L)
- OpenAlex
- W1981888677
- Cited by
- 294
- References
- 51
- Citations by year
- 2026: 2 · 2025: 16 · 2024: 21 · 2023: 33 · 2022: 27 · 2021: 27 · 2020: 32 · 2019: 25 · 2018: 9 · 2017: 29 · 2016: 14 · 2015: 20 · 2014: 17 · 2013: 12 · 2012: 5
- FWCI
- 2.87
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- Locations
- Experimental Cell Research (publishedVersion)
- PubMed (publishedVersion)
- CINECA IRIS Institutional Research information system (University of Urbino) (submittedVersion)
- Padua Research Archive (University of Padova) (submittedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Extracellular vesicles in disease
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Muscle Physiology and Disorders
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Caveolin-1 and cellular processes
- Keywords
- exosomes, C2C12 myoblasts, mitochondrial DNA, signal transduction proteins, TSG101, Alix, IGFBP-5, IGF-1 signaling, intercellular communication, skeletal muscle, exosomal proteome, skeletal muscle disorders
- MeSH terms
- Tsg101 Protein, Animals, Calcium-Binding Proteins, Cells, Cultured, DNA-Binding Proteins, DNA, Mitochondrial, Insulin-Like Growth Factor I, Transcription Factors, Signal Transduction, Insulin-Like Growth Factor Binding Protein 5, Myoblasts, Skeletal, Microscopy, Electron, Transmission, Mice, Endosomal Sorting Complexes Required for Transport
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