Differences in growth properties of endometrial cancer in three dimensional (3D) culture and 2D cell monolayer
Abstract
Three-dimensional (3D) in vitro models have an invaluable role in understanding the behaviour of tumour cells in a well defined microenvironment. This is because some aspects of tumour characteristics cannot be fully recapitulated in a cell monolayer (2D). In the present study, we compared growth patterns, expression of signalling molecules, and metabolism-associated proteins of endometrial cancer cell lines in 3D and 2D cell cultures. Cancer cells formed spherical structures in 3D reconstituted basement membrane (3D rBM), and the morphological appearance was cell line dependent. Cell differentiation was observed after 8 days in the 3D rBM. There was reduced proliferation, detected by less expression of PCNA in 3D rBM than in 2D cell monolayers. The addition of exogenous epidermal growth factor (EGF) to cancer cells induced phosphorylation of EGFR and Akt in both cell culture conditions. The uptake of glucose was selectively altered in the 3D rBM, but there was a lack of association with Glut-1 expression. The secretion of vascular endothelial growth factor (VEGF) and prostaglandin E(2) (PGE(2)) was selectively altered in 3D rBM, and it was cell line dependent. Our data demonstrated that 3D rBM as an in vitro model can influence proliferation and metabolism of endometrial cancer cell behaviour compared to 2D cell monolayer. Changes are specific to individual cell types. The use of 3D rBM is, therefore, important in the in vitro study of targeted anticancer therapies.
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Overall, the morphological appearance of 3D spheroids is primarily cell line dependent.
“Overall, the morphological appearance of 3D spheroids is primarily cell line dependent. 29,35,37”
From Three-dimensional cell culture systems and their applications in drug discovery and cell-based biosensors (2014) · cites this paper as [37]
Cancer cells cultured in 2D conditions undergo different phenotypes, gene and protein expressions, and drug responses from those in vivo.
“Such is particularly evidenced by cancer cells cultured in 2D conditions undergoing different phenotypes,[ 4 ] gene and protein expressions,[ 5 ] and drug responses[ 6 ]”
From 3D Biomimetic Models to Reconstitute Tumor Microenvironment In Vitro: Spheroids, Organoids, and Tumor‐on‐a‐Chip (2023) · cites this paper as [6]
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- Authors
- Chitcholtan, K., Asselin, E., Parent, S., Sykes, P. H., & Evans, J. J.
- Affiliations
- University of Otago (NZ)
- Université du Québec à Trois-Rivières (CA)
- Corresponding author
- Chitcholtan, Kenny
- Venue
- Experimental Cell Research
- Publisher
- Elsevier BV
- Published
- 2013-01
- Volume
- 319
- Issue
- 1
- Pages
- 75-87
- ISSN
- 0014-4827 (ISSN-L)
- OpenAlex
- W2078088491
- Cited by
- 173
- References
- 53
- Citations by year
- 2026: 4 · 2025: 11 · 2024: 11 · 2023: 18 · 2022: 12 · 2021: 17 · 2020: 20 · 2019: 12 · 2018: 11 · 2017: 16 · 2016: 13 · 2015: 15 · 2014: 8 · 2013: 5
- FWCI
- 4.43
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Experimental Cell Research (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Oncology › Cancer Cells and Metastasis
- Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
- Keywords
- endometrial cancer, 3D cell culture, reconstituted basement membrane, tumor microenvironment, cell proliferation, PCNA, epidermal growth factor, EGFR phosphorylation, Akt phosphorylation, glucose uptake, GLUT1, vascular endothelial growth factor, prostaglandin E2, cell differentiation, cell line dependence
- MeSH terms
- Cell Differentiation, Female, Humans, Signal Transduction, Endometrial Neoplasms, Cell Culture Techniques, Cell Line, Tumor, Cell Proliferation, Tumor Microenvironment
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