Salutary effects of attenuation of angiotensin II on coronary perivascular fibrosis associated with insulin resistance and obesity
Abstract
Obesity and insulin resistance confer increased risk for accelerated coronary disease and cardiomyopathic phenomena. We have previously shown that inhibition of angiotensin-converting enzyme (ACE) prevents coronary perimicrovascular fibrosis in genetically obese mice that develop insulin resistance. This study was performed to elucidate mechanism(s) implicated and to determine the effects of attenuation of angiotensin II (Ang) II. Genetically obese ob/ob mice were given ACE inhibitor (temocapril) or Ang II type 1 (AT(1)) receptor blocker (olmesartan) from 10 to 20 weeks. Cardiac expressions of plasminogen activator inhibitor (PAI)-1, the major physiologic inhibitor of fibrinolysis, and transforming growth factor (TGF)-beta(1), a prototypic profibrotic molecule, were determined and extent of perivascular coronary fibrosis was measured. Twenty-week-old obese mice exhibited increased plasma levels of PAI-1 and TGF-beta(1) compared with the values in lean counterpart. Perivascular coronary fibrosis in arterioles and small arteries was evident in obese mice that also showed increased left ventricular collagen as measured by hydroxyproline assay. Immunohistochemistry confirmed the deposition of perivascular type 1 collagen. Markedly increased PAI-1 and TGF-beta were seen immunohistochemically in coronary vascular wall and confirmed by western blotting. When obese mice were treated with temocapril or olmesartan from 10 to 20 weeks, both were equally effective and prevented increases in perivascular fibrosis, plasma PAI-1 and TGF-beta(1), left ventricular collagen and mural immunoreactivity for PAI-1, TGF-beta and collagen type 1. The c-Jun NH(2)-terminal kinase (JNK) activity was elevated in the left ventricle of obese mice (western) and blocked by temocapril and olmesartan. Ang II-mediated upregulation of PAI-1 and TGF-beta(1) with collagen deposition may explain the mechanism of perivascular fibrosis in obese mice. ACE inhibition and blockade of AT(1) receptor may prevent coronary perivascular fibrosis and collagen deposition even before development of overt diabetes. JNK activation may be a mediator of obesity-related cardiac dysfunction and a potential therapeutic target.
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In ob/ob mice, fibrosis occurs mainly in the coronary perivascular region.
“In ob/ob mice, fibrosis occurs mainly in the coronary perivascular region (391, 392).”
From Cardiac remodeling in obesity (2008) · cites this paper as [391]
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- Authors
- Zaman, A. K. M. T., Fujii, S., Goto, D., Furumoto, T., Mishima, T., Nakai, Y., Dong, J., Imagawa, S., Sobel, B. E., & Kitabatake, A.
- Affiliations
- Hokkaido University (JP)
- University of Vermont (US)
- Corresponding author
- Fujii, Satoshi
- Venue
- Journal of Molecular and Cellular Cardiology
- Publisher
- Elsevier BV
- Published
- 2004-08
- Volume
- 37
- Issue
- 2
- Pages
- 525-535
- ISSN
- 0022-2828 (ISSN-L)
- OpenAlex
- W2116549196
- Cited by
- 70
- References
- 42
- Citations by year
- 2026: 2 · 2025: 1 · 2021: 2 · 2020: 1 · 2019: 1 · 2018: 4 · 2017: 2 · 2016: 2 · 2015: 6 · 2014: 3 · 2013: 9 · 2012: 3
- FWCI
- 1.96
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Molecular and Cellular Cardiology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Ministry of Education, Culture, Sports, Science and Technology
- Topics
- Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Renin-Angiotensin System Studies
- Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Cardiac Fibrosis and Remodeling
- Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Cardiovascular Disease and Adiposity
- Keywords
- angiotensin II, insulin resistance, obesity, angiotensin-converting enzyme inhibition, angiotensin II type 1 receptor blockade, temocapril, olmesartan, plasminogen activator inhibitor-1, TGF-β1, c-Jun NH2-kinase, ob/ob mice, cardiomyopathy
- MeSH terms
- Olmesartan Medoxomil, Angiotensin II, Angiotensin-Converting Enzyme Inhibitors, Animals, Blood Glucose, Coronary Disease, Coronary Vessels, Fibrosis, Heart Ventricles, Imidazoles, Insulin, Insulin Resistance, Male, Mice, Obese, Obesity, Phosphorylation, Tetrazoles, Thiazepines, Transforming Growth Factor beta, Plasminogen Activator Inhibitor 1, Matrix Metalloproteinase 9, Collagen Type I, Angiotensin II Type 1 Receptor Blockers, JNK Mitogen-Activated Protein Kinases, Mice
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