The DOI matches its Crossref record; 36 of the 36 references it cites were found in a registry or an index.
MicroRNA-21 protects against the H2O2-induced injury on cardiac myocytes via its target gene PDCD4
Open accessAbstract
Reactive oxygen species (ROS)-induced cardiac cell injury via expression changes of multiple genes plays a critical role in the pathogenesis of numerous heart diseases. MicroRNAs (miRNAs) comprise a novel class of endogenous, small, noncoding RNAs that negatively regulate about 30% of the genes in a cell via degradation or translational inhibition of their target mRNAs. Currently, the effects of ROS on miRNA expression and the roles of miRNAs in ROS-mediated injury on cardiac myocytes are uncertain. Using quantitative real-time RT-PCR (qRT-PCR), we demonstrated that microRNA-21 (miR-21) was upregulated in cardiac myocytes after treatment with hydrogen peroxide (H2O2). To determine the potential roles of miRNAs in H2O2-mediated gene regulation and cellular injury, miR-21 expression was downregulated by miR-21 inhibitor and upregulated by pre-miR-21. H2O2-induced cardiac cell death and apoptosis were increased by miR-21 inhibitor and was decreased by pre-miR-21. Programmed cell death 4 (PDCD4) that was regulated by miR-21 and was a direct target of miR-21 in cardiac myocytes. Pre-miR-21-mediated protective effect on cardiac myocyte injury was inhibited in H2O2-treated cardiac cells via adenovirus-mediated overexpression of PDCD4 without miR-21 binding site. Moreover, Activator protein 1 (AP-1) was a downstream signaling molecule of PDCD4 that was involved in miR-21-mediated effect on cardiac myocytes. The results suggest that miR-21 is sensitive to H2O2 stimulation. miR-21 participates in H2O2-mediated gene regulation and functional modulation in cardiac myocytes. miR-21 might play an essential role in heart diseases related to ROS such as cardiac hypertrophy, heart failure, myocardial infarction, and myocardial ischemia/reperfusion injury.
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“For example, miR-21 protects cardiomyocytes against H2O2 induced cell apoptosis by targeting the proapoptotic proteins PDCD437.”
From Long non-coding RNA MEG3 functions as a competing endogenous RNA to regulate ischemic neuronal death by targeting miR-21/PDCD4 signaling pathway (2017) · cites this paper as [37]
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“Cheng et al. have shown that miR-21 is upregulated in response to H2O2 injury in cardiac myocytes, and that it functions to attenuate apoptosis in that cell population [29].”
From MicroRNA‐21 protects neurons from ischemic death (2010) · cites this paper as [29]
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Bartel (2004) · doi:10.1016/s0092-8674(04)00045-5 · Cited by 34,714
Lewis, B. P., Burge, C. B., & Bartel, D. P. (2005) · Cell · doi:10.1016/j.cell.2004.12.035 · Cited by 11,855
MicroRNA-21 contributes to myocardial disease by stimulating MAP kinase signalling in fibroblasts
Thum, T., Gross, C., Fiedler, J., Fischer, T., Kissler, S., Bussen, M., Galuppo, P., Just, S., Rottbauer, W., Frantz, S., et al. (2008) · Nature · doi:10.1038/nature07511 · Cited by 2,349
Asangani, I. A., Rasheed, S. A. K., Nikolova, D. A., Leupold, J. H., Colburn, N. H., Post, S., & Allgayer, H. (2008) · Oncogene · doi:10.1038/sj.onc.1210856 · Cited by 1,882
Identification of hundreds of conserved and nonconserved human microRNAs
Bentwich, I., Avniel, A., Karov, Y., Aharonov, R., Gilad, S., Barad, O., Barzilai, A., Einat, P., Einav, U., Meiri, E., et al. (2005) · Nature Genetics · doi:10.1038/ng1590 · Cited by 1,841
AP-1 in cell proliferation and survival
Shaulian, E., & Karin, M. (2001) · Oncogene · doi:10.1038/sj.onc.1204383 · Cited by 1,733
The Widespread Impact of Mammalian MicroRNAs on mRNA Repression and Evolution
Farh, K. K.-H., Grimson, A., Jan, C., Lewis, B. P., Johnston, W. K., Lim, L. P., Burge, C. B., & Bartel, D. P. (2005) · Science · doi:10.1126/science.1121158 · Cited by 1,484
Phylogenetic Shadowing and Computational Identification of Human microRNA Genes
Berezikov, E., Guryev, V., van de Belt, J., Wienholds, E., Plasterk, R. H. A., & Cuppen, E. (2005) · Cell · doi:10.1016/j.cell.2004.12.031 · Cited by 1,270
MicroRNA Pathways in Flies and Worms
Ambros, V. (2003) · Cell · doi:10.1016/s0092-8674(03)00428-8 · Cited by 1,218
Frankel, L. B., Christoffersen, N. R., Jacobsen, A., Lindow, M., Krogh, A., & Lund, A. H. (2008) · Journal of Biological Chemistry · doi:10.1074/jbc.m707224200 · Cited by 1,141
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Biological functions of microRNAs: a review
Huang, Y., Shen, X. J., Zou, Q., Wang, S. P., Tang, S. M., & Zhang, G. Z. (2010) · Journal of Physiology and Biochemistry · doi:10.1007/s13105-010-0050-6 · Cited by 659
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Small, E. M., Frost, R. J., & Olson, E. N. (2010) · Circulation · doi:10.1161/circulationaha.109.889048 · Cited by 604
Cardiac Fibrosis in Patients With Atrial Fibrillation
Dzeshka, M. S., Lip, G. Y. H., Snezhitskiy, V. А., & Shantsila, E. (2015) · Journal of the American College of Cardiology · doi:10.1016/j.jacc.2015.06.1313 · Cited by 564
Dong, S., Cheng, Y., Yang, J., Li, J., Liu, X., Wang, X., Wang, D., Krall, T. J., Delphin, E. S., & Zhang, C. (2009) · Journal of Biological Chemistry · doi:10.1074/jbc.m109.027896 · Cited by 454
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Metadata
- Authors
- Cheng, Y., Liu, X., Zhang, S., Lin, Y., Yang, J., & Zhang, C.
- Affiliations
- Rutgers New Jersey Medical School (US)
- Corresponding author
- Zhang, Chunxiang
- Venue
- Journal of Molecular and Cellular Cardiology
- Publisher
- Elsevier BV
- Published
- 2009-07
- Volume
- 47
- Issue
- 1
- Pages
- 5-14
- ISSN
- 0022-2828 (ISSN-L)
- PMC
- PMC3593965
- OpenAlex
- W2141570739
- Semantic Scholar
- 1fbe4e1a56db9230d95dce535ef1d9e12a4d760a
- Cited by
- 368
- References
- 36
- Citations by year
- 2026: 8 · 2025: 9 · 2024: 14 · 2023: 11 · 2022: 16 · 2021: 20 · 2020: 24 · 2019: 25 · 2018: 23 · 2017: 18 · 2016: 29 · 2015: 23 · 2014: 26 · 2013: 28 · 2012: 29
- FWCI
- 10.27
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Journal of Molecular and Cellular Cardiology (publishedVersion)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- Funders
- National Institutes of Health
- National Heart, Lung, and Blood Institute (HL 080133, R01HL095707, R01HL080133)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › MicroRNA in disease regulation
- Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Cardiac Ischemia and Reperfusion
- Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Cardiac Fibrosis and Remodeling
- Keywords
- miR-21, PDCD4, hydrogen peroxide, reactive oxygen species, cardiomyocytes, oxidative stress, apoptosis, cardioprotection, AP-1, microRNA target regulation, heart disease
- MeSH terms
- Animals, Hydrogen Peroxide, Necrosis, Blotting, Western, Rats, Sprague-Dawley, Apoptosis, Transcription Factor AP-1, Reverse Transcriptase Polymerase Chain Reaction, Myocytes, Cardiac, MicroRNAs, Apoptosis Regulatory Proteins, Rats
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