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Identification of hundreds of conserved and nonconserved human microRNAs

Bentwich, Isaac, Avniel, Amir, Karov, Yael, Aharonov, Ranit, Gilad, Shlomit, Barad, Omer, Barzilai, Adi, Einat, Paz +5 more·2005·Nature Genetics
DOI 10.1038/ng1590
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Abstract

MicroRNAs are noncoding RNAs of approximately 22 nucleotides that suppress translation of target genes by binding to their mRNA and thus have a central role in gene regulation in health and disease. To date, 222 human microRNAs have been identified, 86 by random cloning and sequencing, 43 by computational approaches and the rest as putative microRNAs homologous to microRNAs in other species. To prove our hypothesis that the total number of microRNAs may be much larger and that several have emerged only in primates, we developed an integrative approach combining bioinformatic predictions with microarray analysis and sequence-directed cloning. Here we report the use of this approach to clone and sequence 89 new human microRNAs (nearly doubling the current number of sequenced human microRNAs), 53 of which are not conserved beyond primates. These findings suggest that the total number of human microRNAs is at least 800.

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  • Computational predictions estimate that vertebrate genomes may contain up to ~1000 miRNA genes.

    “while computational predictions estimate that the vertebrate genomes may contain up to z1000 miRNA genes (Bentwich et al. 2005;Berezikov et al. 2005).”

    From MicroRNA expression in the adult mouse central nervous system (2008) · cites this paper as Bentwich et al. 2005

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Authors
Bentwich, I., Avniel, A., Karov, Y., Aharonov, R., Gilad, S., Barad, O., Barzilai, A., Einat, P., Einav, U., Meiri, E., Sharon, E., Spector, Y., & Bentwich, Z.
Venue
Nature Genetics
Publisher
Springer Science and Business Media LLC
Published
2005-06-19
Volume
37
Issue
7
Pages
766-770
ISSN
  • 1061-4036
  • 1546-1718
DOI
10.1038/ng1590
Cited by
1,465
References
1
Language
en
Licence
www.springer.com/tdm (tdm)

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