The DOI matches its Crossref record; 4 of the 4 references it cites were found in a registry or an index.
Detecting HIV-1 integration by repetitive-sampling -gag PCR
Open accessAbstract
In this review, we compare four assays that are currently used to measure HIV integration and discuss their strengths and weaknesses. We then outline advances that have been made toward development of a more robust, more sensitive, quantitative HIV integration assay suitable for clinical use. The assay that we have developed uses repetitive-sampling Alu-gag PCR. The detailed protocol describes our assay step-by-step, the creation of an integration standard cell line and accompanying standard curve, as well as the quantitation of integration and calculation of associated error estimates. Finally, we speculate on fundamental, unresolved issues in HIV latency that can be addressed by measuring HIV integration.
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Cites
Quantification of latent tissue reservoirs and total body viral load in HIV-1 infection
Chun, T.-W., Carruth, L., Finzi, D., Shen, X., DiGiuseppe, J. A., Taylor, H., Hermankova, M., Chadwick, K., Margolick, J., Quinn, T. C., et al. (1997) · Nature · doi:10.1038/387183a0 · Cited by 2,050
Identification of host proteins required for HIV infection through a functional genomic screen
Brass, A. L., Dykxhoorn, D. M., Benita, Y., Yan, N., Engelman, A., Xavier, R. J., Lieberman, J., & Elledge, S. J. (2008) · Science · doi:10.1126/science.1152725 · Cited by 1,449
Multiple effects of mutations in human immunodeficiency virus type 1 integrase on viral replication
Engelman, A., Englund, G., Orenstein, J. M., Martin, M. A., & Craigie, R. (1995) · Journal of Virology · doi:10.1128/jvi.69.5.2729-2736.1995 · Cited by 445
Swiggard, W. J., Baytop, C., Yu, J. J., Dai, J., Li, C., Schretzenmair, R., Theodosopoulos, T., & O'Doherty, U. (2005) · Journal of Virology · doi:10.1128/jvi.79.22.14179-14188.2005 · Cited by 182
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Cited by
Comparative Analysis of Measures of Viral Reservoirs in HIV-1 Eradication Studies
Eriksson, S., Graf, E. H., Dahl, V., Strain, M. C., Yukl, S. A., Lysenko, E. S., Bosch, R. J., Lai, J., Chioma, S., Emad, F., et al. (2013) · PLoS Pathogens · doi:10.1371/journal.ppat.1003174 · Cited by 567
Self-citationHIV-1 persistence in CD4+ T cells with stem cell–like properties
Buzon, M. J., Sun, H., Li, C., Shaw, A., Seiss, K., Ouyang, Z., Martin-Gayo, E., Leng, J., Henrich, T. J., Li, J. Z., et al. (2014) · Nature Medicine · doi:10.1038/nm.3445 · Cited by 414
Sequential LASER ART and CRISPR Treatments Eliminate HIV-1 in a Subset of Infected Humanized Mice
Dash, P. K., Kaminski, R., Bella, R., Su, H., Mathews, S., Ahooyi, T. M., Chen, C., Mancuso, P., Sariyer, R., Ferrante, P., et al. (2019) · Nature Communications · doi:10.1038/s41467-019-10366-y · Cited by 295
Pace, M. J., Graf, E. H., Agosto, L. M., Mexas, A. M., Male, F., Brady, T., Bushman, F. D., & O'Doherty, U. (2012) · PLoS Pathogens · doi:10.1371/journal.ppat.1002818 · Cited by 144
Self-citationSwaminathan, G., Rossi, F., Sierra, L.-J., Gupta, A., Navas-Martín, S., & Martín-García, J. (2012) · PLoS Pathogens · doi:10.1371/journal.ppat.1002937 · Cited by 103
Metadata
- Authors
- Liszewski, M. K., Yu, J. J., & O’Doherty, U.
- Venue
- Methods
- Publisher
- Elsevier BV
- Published
- 2009-04
- Volume
- 47
- Issue
- 4
- Pages
- 254-260
- ISSN
- 1046-2023 (ISSN-L)
- OpenAlex
- W2123943261
- Cited by
- 158
- References
- 4
- FWCI
- 2.47
- Type
- journal article
- Language
- en
- Open access
- green
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- Liszewski, M. K., Yu, J. J., & O’Doherty, U. (2009). Detecting HIV-1 integration by repetitive-sampling -gag PCR. Methods. https://doi.org/10.1016/j.ymeth.2009.01.002
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.