Cited by 2,050HIV Research and TreatmentHIV/AIDS drug development and treatmentInfectious Diseases and Tuberculosis

Quantification of latent tissue reservoirs and total body viral load in HIV-1 infection

Chun, Tae-Wook, Carruth, Lucy, Finzi, Diana, Shen, Xuefei, DiGiuseppe, Joseph A., Taylor, Harry, Hermankova, Monika, Chadwick, Karen +7 more·1997·Nature

Abstract

The capacity of HIV-1 to establish latent infection of CD4+ T cells may allow viral persistence despite immune responses and antiretroviral therapy. Measurements of infectious virus and viral RNA in plasma and of infectious virus, viral DNA and viral messenger RNA species in infected cells all suggest that HIV-1 replication continues throughout the course of infection. Uncertainty remains over what fraction of CD4+ T cells are infected and whether there are latent reservoirs for the virus. We show here that during the asymptomatic phase of infection there is an extremely low total body load of latently infected resting CD4+ T cells with replication-competent integrated provirus (<10(7) cells). The most prevalent form of HIV-1 DNA in resting and activated CD4+ T cells is a full-length, linear, unintegrated form that is not replication competent. The infection progresses even though at any given time in the lymphoid tissues integrated HIV-1 DNA is present in only a minute fraction of the susceptible populations, including resting and activated CD4+ T cells and macrophages.

Integrity signals

Registry

Crossref · 10.1038/387183a0

Cites

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  • HAART profoundly reduces HIV-1 replication and plasma viremia but does not suppress low-level viral genome expression and replication in tissues or target the latently infected cells that serve as an HIV-1 reservoir.

    “HAART profoundly reduces viral replication in cells that support HIV-1 infection and reduces plasma viremia to a minimal level but neither suppresses low-level viral genome expression and replication in tissues nor targets the latently infected cells that serve as a reservoir for HIV-1”

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Authors
Chun, T.-W., Carruth, L., Finzi, D., Shen, X., DiGiuseppe, J. A., Taylor, H., Hermankova, M., Chadwick, K., Margolick, J., Quinn, T. C., Kuo, Y.-H., Brookmeyer, R., Zeiger, M. A., Barditch-Crovo, P., & Siliciano, R. F.
Affiliations
  • Johns Hopkins University (US)
  • National Institutes of Health (US)
Venue
Nature
Published
1997-05
Volume
387
Issue
6629
Pages
183-188
ISSN
  • 0028-0836 (ISSN-L)
  • 1476-4687
OpenAlex
W2030844138
Cited by
2,050
References
29
Citations by year
2026: 29 · 2025: 47 · 2024: 48 · 2023: 59 · 2022: 54 · 2021: 56 · 2020: 80 · 2019: 79 · 2018: 102 · 2017: 81 · 2016: 98 · 2015: 79 · 2014: 86 · 2013: 87 · 2012: 74
FWCI
25.32
Type
journal article
Language
en
Open access
closed
Locations
  • Nature (publishedVersion)
  • PubMed (publishedVersion)
  • RePEc: Research Papers in Economics (submittedVersion)
Topics
  • Life Sciences › Immunology and Microbiology › Virology › HIV Research and Treatment
  • Health Sciences › Medicine › Infectious Diseases › HIV/AIDS drug development and treatment
  • Health Sciences › Medicine › Surgery › Infectious Diseases and Tuberculosis
Keywords
HIV-1, latent viral reservoir, CD4+ T cells, integrated provirus, viral persistence, asymptomatic HIV infection, activated T cells, macrophages, viral DNA, viral RNA, antiretroviral therapy
MeSH terms
Cells, Cultured, DNA, Viral, Humans, Immunologic Memory, Lymph Nodes, Lymphocyte Activation, Macrophages, Proviruses, Virus Replication, CD4-Positive T-Lymphocytes, HIV-1, HIV Infections, Polymerase Chain Reaction, Virus Integration, Leukocyte Common Antigens, Virus Latency, Coculture Techniques, Anti-HIV Agents, Viral Load

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