Quantitative analysis of cellular senescence phenotypes using an imaging cytometer
Abstract
Until now, various stimuli as well as serial passaging have been known to induce cellular senescence in normal human diploid fibroblasts. However, in many cases, we have encountered difficulty in quantitatively analyzing the cellular senescence phenotypes of senescent cells in a physiological condition. High-content screening (HCS)-based image analysis is becoming an important and powerful research tool. In the present study, an automated and quantitative cellular image-analysis system was employed to quantify the cellular senescence phenotypes induced in normal human diploid fibroblasts, TIG-1 cells, and found to be a powerful tool in the cellular senescence study.
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STAT3 is essential for both the early ROS increase and the later senescence-associated-β-galactosidase activity through STAT3-regulated soluble factor IGFBP5 in human fibroblasts and vascular endothelial cells.
“STAT3 was similarly described to be essential for both the early ROS increase, as well as the later senescence-associated-β-galactosidase activity, through STAT3-regulated soluble factor insulin-like growth factor binding protein 5 in both human fibroblasts (Udono et al., 2012) and vascular endothelial cells (Kim et al., 2007).”
From Targeting Janus Kinases and Signal Transducer and Activator of Transcription 3 To Treat Inflammation, Fibrosis, and Cancer: Rationale, Progress, and Caution (2020) · cites this paper as Udono et al., 2012
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Rb-mediated heterochromatin formation and silencing of E2F target genes during cellular senescence
Narita, M., Nuñez, S., Heard, E., Narita, M., Lin, A. W., Hearn, S. A., Spector, D. L., Hannon, G. J., & Lowe, S. W. (2003) · Cell · doi:10.1016/s0092-8674(03)00401-x · Cited by 2,278 · see citing works
Kurz, D. J., Decary, S., Hong, Y., & Erusalimsky, J. D. (2000) · Journal of Cell Science · doi:10.1242/jcs.113.20.3613 · Cited by 899 · see citing works
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Bharadwaj, U., Kasembeli, M. M., Robinson, P., & Tweardy, D. J. (2020) · Pharmacological Reviews · doi:10.1124/pr.119.018440 · Cited by 314 · see citing works
“…to be essential for both the early ROS increase, as well as the later senescence-associated-b-galactosidase activity, through STAT3-regulated soluble factor insulinlike growth factor binding protein 5 in both human fibroblasts (Udono et al., 2012) and vascular endothelial cells (Kim et al., 2007).”
BackgroundIL-6-STAT3 signaling and premature senescence
Kojima, H., Inoue, T., Kunimoto, H., & Nakajima, K. (2013) · JAK-STAT · doi:10.4161/jkst.25763 · Cited by 170 · see citing works
- Authors
- Udono, M., Kadooka, K., Yamashita, S., & Katakura, Y.
- Affiliations
- Kyushu University (JP)
- ORCID
- Katakura, Yoshinori
- Venue
- Methods
- Publisher
- Elsevier BV
- Published
- 2012-03
- Volume
- 56
- Issue
- 3
- Pages
- 383-388
- ISSN
- 1046-2023 (ISSN-L)
- OpenAlex
- W2065006123
- Cited by
- 22
- References
- 2
- Citations by year
- 2026: 2 · 2022: 1 · 2020: 2 · 2019: 1 · 2018: 4 · 2017: 2 · 2016: 2 · 2015: 3 · 2014: 3 · 2013: 2
- FWCI
- 1.72
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Methods (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Japan Society for the Promotion of Science (24580190)
- Topics
- Health Sciences › Medicine › Physiology › Telomeres, Telomerase, and Senescence
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Cell Image Analysis Techniques
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Aging › Genetics, Aging, and Longevity in Model Organisms
- Keywords
- cellular senescence, image cytometry, high-content screening, image analysis, human diploid fibroblasts, senescence induction, quantitative analysis
- MeSH terms
- Cells, Cultured, Humans, Phenotype, Cellular Senescence, Image Cytometry
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