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Cited by 47Advanced Proteomics Techniques and ApplicationsMass Spectrometry Techniques and ApplicationsMetabolomics and Mass Spectrometry Studies

Development of multiplex mass spectrometric immunoassay for detection and quantification of apolipoproteins C-I, C-II, C-III and their proteoforms

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Trenchevska, Olgica, Schaab, Matthew R., Nelson, Randall W., Nedelkov, Dobrin·2015·Methods
DOI 10.1016/j.ymeth.2015.02.020PMC PMC4574700
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Abstract

The impetus for discovery and evaluation of protein biomarkers has been accelerated by recent development of advanced technologies for rapid and broad proteome analyses. Mass spectrometry (MS)-based protein assays hold great potential for in vitro biomarker studies. Described here is the development of a multiplex mass spectrometric immunoassay (MSIA) for quantification of apolipoprotein C-I (apoC-I), apolipoprotein C-II (apoC-II), apolipoprotein C-III (apoC-III) and their proteoforms. The multiplex MSIA assay was fast (∼ 40 min) and high-throughput (96 samples at a time). The assay was applied to a small cohort of human plasma samples, revealing the existence of multiple proteoforms for each apolipoprotein C. The quantitative aspect of the assay enabled determination of the concentration for each proteoform individually. Low-abundance proteoforms, such as fucosylated apoC-III, were detected in less than 20% of the samples. The distribution of apoC-III proteoforms varied among samples with similar total apoC-III concentrations. The multiplex analysis of the three apolipoproteins C and their proteoforms using quantitative MSIA represents a significant step forward toward better understanding of their physiological roles in health and disease.

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Cited by

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Authors
Trenchevska, O., Schaab, M. R., Nelson, R. W., & Nedelkov, D.
Affiliations
Arizona State University (US)
Corresponding author
Nedelkov, Dobrin
Venue
Methods
Publisher
Elsevier BV
Published
2015-06
Volume
81
Pages
86-92
ISSN
1046-2023 (ISSN-L)
DOI
10.1016/j.ymeth.2015.02.020
PMC
PMC4574700
OpenAlex
W2081058730
Cited by
47
References
4
Citations by year
2026: 1 · 2024: 5 · 2021: 2 · 2020: 4 · 2019: 4 · 2018: 6 · 2017: 7 · 2016: 13 · 2015: 5
FWCI
4.26
Type
journal article
Language
en
Open access
Licence
www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Locations
  • Methods (publishedVersion)
  • PubMed (publishedVersion)
  • Europe PMC (PubMed Central) (acceptedVersion)
  • PubMed Central (acceptedVersion)
  • Arizona State University Library Digital Repository (Arizona State University) (acceptedVersion, cc-by-nc-nd)
Funders
National Institute of Diabetes and Digestive and Kidney Diseases (R01DK082542, R24DK090958)
Topics
  • Physical Sciences › Chemistry › Spectroscopy › Advanced Proteomics Techniques and Applications
  • Physical Sciences › Chemistry › Spectroscopy › Mass Spectrometry Techniques and Applications
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Metabolomics and Mass Spectrometry Studies
Keywords
apolipoprotein C-I, apolipoprotein C-II, apolipoprotein C-III, proteoforms, protein biomarkers, plasma apolipoproteins
MeSH terms
Humans, Immunoassay, Protein Processing, Post-Translational, Mass Spectrometry, Protein Isoforms, Apolipoprotein C-I, Apolipoprotein C-II, Apolipoprotein C-III

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