Cited by 442Advanced Fluorescence Microscopy TechniquesNonlinear Optical Materials StudiesLaser-Matter Interactions and Applications

The DOI matches its Crossref record; 26 of the 27 references it cites were found in a registry or an index.

Highly Nonlinear Photodamage in Two-Photon Fluorescence Microscopy

Open access

Hopt, Alexander, Neher, Erwin·2001·Biophysical Journal

DOI 10.1016/s0006-3495(01)76173-5PMC PMC1301392

Open this paper in CoChat — free account Open PDF

Abstract

Two-photon fluorescence excitation is being increasingly used in laser scan microscopy due to very low photodamage induced by this technique under normal operation. However, excitation intensity has to be kept low, because nonlinear photodamage sets in when laser power is increased above a certain threshold. We studied this kind of damage in bovine adrenal chromaffin cells, using two different indicators of damage: changes in resting [Ca(2+)] level and the degranulation reaction. In agreement with previous studies, we found that, for both criteria, damage is proportional to the integral (over space and time) of light intensity raised to a power approximately 2.5. Thus, widening the laser pulse shape at constant average intensity both in time and in focal volume is beneficial for avoiding this kind of damage. Both measures, of course, reduce the two-photon fluorescence excitation. However, loss of signal can be compensated by increasing excitation power, such that, at constant damaging potential, signals may be even larger with long pulses and large focal volumes, because the exponent of the power law of damage is higher (mu approximately 2.5) than that of the two-photon signal (mu approximately 2).

Integrity signals

Registry

Crossref · 10.1016/s0006-3495(01)76173-5

Cites

26 of 26 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

We couldn’t retrieve this paper’s full text, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Two-photon laser scanning fluorescence microscopy
Denk, W., Strickler, J. H., & Webb, W. W. (1990) · Science · doi:10.1126/science.2321027 · Cited by 9,488
Handbook of fluorescent probes and research chemicals
Haugland, R. P. (1996) · Medical Entomology and Zoology · Cited by 2,793
Measurement of two-photon excitation cross sections of molecular fluorophores with data from 690 to 1050 nm
Xu, C., & Webb, W. W. (1996) · Journal of the Optical Society of America B · doi:10.1364/josab.13.000481 · Cited by 2,198
Ultrashort Laser Pulse Phenomena
Diels, J., & Rudolph, W. (2006) · Elsevier eBooks · doi:10.1016/b978-0-12-215493-5.x5000-9 · Cited by 853
Theory and Practice of Scanning Optical Microscopy
Dainty, J. C. (1985) · Optica Acta International Journal of Optics · doi:10.1080/713821694 · Cited by 773
Introduction
Lynch, D. K. (1987) · Journal of the Optical Society of America A · doi:10.1364/josaa.4.000558 · Cited by 756
Photon Upmanship: Why Multiphoton Imaging Is More than a Gimmick
Denk, W. T., & Svoboda, K. (1997) · Neuron · doi:10.1016/s0896-6273(00)81237-4 · Cited by 679
Breakdown threshold and plasma formation in femtosecond laser–solid interaction
von der Linde, D., & Schüler, H. (1996) · Journal of the Optical Society of America B · doi:10.1364/josab.13.000216 · Cited by 308
Ca2+ Fluorescence Imaging with Pico- and Femtosecond Two-Photon Excitation: Signal and Photodamage
Koester, H. J., Baur, D., Uhl, R., & Hell, S. W. (1999) · Biophysical Journal · doi:10.1016/s0006-3495(99)77063-3 · Cited by 265
Pulse-length dependence of cellular response to intense near-infrared laser pulses in multiphoton microscopes
König, K., Becker, T., Fischer, P., Riemann, I., & Halbhuber, K. J. (1999) · Optics Letters · doi:10.1364/ol.24.000113 · Cited by 259

Show all 27 references

Research works printed but not linked: 2

Clayton, 1977, Vol. 1 and 2
Ridsdale, 1993, The viability of cultured cells under two-photon laser scanning microscopy (abstr.), Biophys. J., 64, A109

Sorted by citations

Items 301–400 of 441

An Introduction to In Vitro Slice Approaches for the Study of Neuronal Circuitry
Varela, C., Llano, D. A., & Theyel, B. B. (2011) · Neuromethods · doi:10.1007/7657_2011_19 · Cited by 8
Novel approach to real-time flash photolysis and confocal [Ca2+] imaging
Sobie, E. A., Kao, J. P. Y., & Lederer, W. J. (2007) · Pflügers Archiv - European Journal of Physiology · doi:10.1007/s00424-007-0229-z · Cited by 9
Two-photon imaging induces brain heating and calcium microdomain hyperactivity in cortical astrocytesPreprint
Schmidt, E. M., & Oheim, M. (2018) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/321091 · Cited by 9
Divergent excitation two photon microscopy for 3D random access mesoscale imaging at single cell resolutionPreprint
Janiak, F. K., Bartel, P., Bale, M. R., Yoshimatsu, T., Komulainen, E., Zhou, M., Staras, K., Prieto-Godino, L. L., Euler, T., Maravall, M., et al. (2019) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/821405 · Cited by 9
A Deep Learning Approach for Improving Two-Photon Vascular Imaging Speeds
Zhou, A., Mihelic, S. A., Engelmann, S. A., Tomar, A., Dunn, A. K., & Narasimhan, V. M. (2024) · Bioengineering · doi:10.3390/bioengineering11020111 · Cited by 8
Vibrational two‐photon microscopy for tissue imaging: Short‐wave infrared surface‐enhanced resonance hyper‐Raman scattering
Olson, J. E., Yu, J. H., Thimes, R. L., & Camden, J. P. (2021) · Journal of Biophotonics · doi:10.1002/jbio.202100158 · Cited by 8
Precision 1070 nm Ultrafast Laser‐Induced Photothrombosis of Depth‐Targeted Vessels In Vivo
Zhu, L., Wang, M., Fu, P., Liu, Y., Zhang, H., Roe, A. W., & Xi, W. (2023) · Small Methods · doi:10.1002/smtd.202200917 · Cited by 8
Multi-dimensional time-correlated single-photon counting
Becker, W. H., & Bergmann, A. (2007) · Proceedings of SPIE, the International Society for Optical Engineering/Proceedings of SPIE · doi:10.1117/12.735546 · Cited by 8
Femtosecond Plasma-Mediated Nanosurgery of Cells and Tissues
Vogel, A., Noack, J., Hüttman, G., & Paltauf, G. (2007) · Springer eBooks · doi:10.1007/978-0-387-30453-3_10 · Cited by 8
Strategies to overcome photobleaching in algorithm-based adaptive optics for nonlinear in-vivo imaging
Müllenbroich, M. C., McGhee, E. J., Wright, A. J., Anderson, K. I., & Mathieson, K. (2014) · Journal of Biomedical Optics · doi:10.1117/1.jbo.19.1.016021 · Cited by 8
Optical parametrically gated microscopy in scattering media
Zhao, Y., Adie, S. G., Tu, H., Liu, Y., Graf, B. W., Chaney, E. J., Marjanović, M., & Boppart, S. A. (2014) · Optics Express · doi:10.1364/oe.22.022547 · Cited by 7
Photodamage in deep tissue two‐photon optical biopsy of human skin
Dalbosco, L., Zanini, G., D’Amato, E., Tessarolo, F., Boi, S., Bauer, P., Haase, A., & Antolini, R. (2014) · Journal of Biophotonics · doi:10.1002/jbio.201400083 · Cited by 7
Simultaneous multi‐spectral, single‐photon fluorescence imaging using a plasmonic colour filter array
Connolly, P. W. R., Valli, J., Shah, Y. D., Altmann, Y., Grant, J. P., Accarino, C., Rickman, C., Cumming, D. R. S., & Buller, G. S. (2021) · Journal of Biophotonics · doi:10.1002/jbio.202000505 · Cited by 7
Genetically encoded fluorescent sensors for redox processes
Bilan, D. S., Lukyanov, S., & Belousov, V. V. (2015) · Russian Journal of Bioorganic Chemistry · doi:10.1134/s106816201502003x · Cited by 7
Enzyme-Dependent Fluorescence Recovery after Photobleaching of NADH: In Vivo and In Vitro Applications to the Study of Enzyme Kinetics
Combs, C. A., & Balaban, R. S. (2004) · Methods in enzymology on CD-ROM/Methods in enzymology · doi:10.1016/s0076-6879(04)85015-8 · Cited by 7
Multiphoton Neurophotonics: Recent Advances in Imaging and Manipulating Neuronal Circuits
Telliez, C., Sims, R. R., Faini, G., Berto, P., Papagiakoumou, E. I., Tanese, D., & Accanto, N. (2025) · ACS Photonics · doi:10.1021/acsphotonics.4c02101 · Cited by 7
Evaluation of compact pulsed lasers for two-photon microscopy using a simple method for measuring two-photon excitation efficiency
Saidi, S., & Shtrahman, M. (2023) · Neurophotonics · doi:10.1117/1.nph.10.4.044303 · Cited by 6
Gentle Label‐Free Nonlinear Optical Imaging Relaxes Linear‐Absorption‐Mediated Triplet
Wang, G., Li, L., Sorrells, J. E., Chen, J., & Tu, H. (2025) · Advanced Science · doi:10.1002/advs.202415648 · Cited by 6
Two-Photon Fluorescence Imaging
Fan, 丰. F., Mao, H., Wang, A., & Chen, L. (2021) · Advances in experimental medicine and biology · doi:10.1007/978-981-15-7627-0_3 · Cited by 6
Ultrafast, Dual-Band Coherent Raman Spectroscopy without Ultrashort Pulses
Hiramatsu, K., Tajima, T., & Goda, K. (2022) · ACS Photonics · doi:10.1021/acsphotonics.2c00768 · Cited by 6
A Comparison of the Sensitivity of Photodamage Assays in Rat Basophilic Leukemia Cells
Barth, E. E., Hallworth, R., & Nichols, M. G. (2005) · Photochemistry and Photobiology · doi:10.1562/2004-08-04-ra-261 · Cited by 6
Multiparametric high‐resolution imaging of barley embryos by multiphoton microscopy and magnetic resonance micro‐imaging
Stark, M., Manz, B., Ehlers, A., Küppers, M., Riemann, I., Volke, F., Siebert, U., Weschke, W., & König, K. (2007) · Microscopy Research and Technique · doi:10.1002/jemt.20426 · Cited by 6
Instrumentation for Live-Cell Imaging and Main Formats
Oheim, M. (2009) · Methods in molecular biology · doi:10.1007/978-1-60761-404-3_1 · Cited by 6
Coherent Raman Tissue Imaging in the BrainPreprint
Saar, B. G., Freudiger, C. W., Xu, X., Huttner, A. J., Kesari, S., Young, G. S., & Xie, X. S. (2014) · Cold Spring Harbor Protocols · doi:10.1101/pdb.top081695 · Cited by 6
Hybrid Iterating-Averaging Low Photon Budget Gabor Holographic Microscopy
Rogalski, M., Arcab, P., Wdowiak, E., Picazo-Bueno, J. Á., Micó, V., Jóźwik, M., & Trusiak, M. (2025) · ACS Photonics · doi:10.1021/acsphotonics.4c01863 · Cited by 5
Compact simultaneous label-free autofluorescence multi-harmonic microscopy for user-friendly photodamage-monitored imaging
Wang, G., Boppart, S. A., & Tu, H. (2024) · Journal of Biomedical Optics · doi:10.1117/1.jbo.29.3.036501 · Cited by 5
Comparing confocal and two-photon Ca2+ imaging of thin low-scattering preparations
Cheng, J., McMahon, S. M., Piston, D. W., & Jackson, M. B. (2023) · Biophysical Reports · doi:10.1016/j.bpr.2023.100109 · Cited by 4
Overview of Supercontinuum Sources for Multiphoton Microscopy and Optical Biopsy
Zhao, Y., & Iftimia, N. V. (2019) · Elsevier eBooks · doi:10.1016/b978-0-323-48067-3.00013-5 · Cited by 5
Broadband 1.5 GHz VECSEL-Based Laser System for High Speed Multicontrast Nonlinear Imaging
Bondaz, T. A. G., McInerney, J. G., Moloney, J. V., & Jones, R. J. (2023) · IEEE Photonics Technology Letters · doi:10.1109/lpt.2023.3261830 · Cited by 5
Ultra‐compact tunable fiber laser for coherent anti‐Stokes Raman imaging
Gottschall, T., Meyer‐Zedler, T., Schmitt, M. P., Huber, R. A., Popp, J., Tünnermann, A., & Limpert, J. (2021) · Journal of Raman Spectroscopy · doi:10.1002/jrs.6171 · Cited by 5
Two-color two-photon excitation of indole using a femtosecond laser regenerative amplifier
Qiao, L., Ni, J., Mao, Z., Wang, C., & Cheng, Y. (2008) · Optics Communications · doi:10.1016/j.optcom.2008.11.034 · Cited by 5
Studying Kinetochores In Vivo Using FLIM-FRET
Yoo, T. Y., & Needleman, D. J. (2016) · Methods in molecular biology · doi:10.1007/978-1-4939-3542-0_11 · Cited by 5
3D STED Microscopy with Pulsed and Continuous Wave Lasers
Harke, B. (2008) · doi:10.53846/goediss-3436 · Cited by 5
Aptamerized silica/gold nanocapsules for stimulated release of doxorubicin through remote two-photon excitation
Tew, L. S., Lee, T.-H., Lo, L., Khung, Y. L., & Chen, N. (2022) · International Journal of Smart and Nano Materials · doi:10.1080/19475411.2022.2033874 · Cited by 5
Fast Diffusion Characterization by Multiphoton Excited Fluorescence Recovery while Photobleaching
Li, M., Razumtcev, A. A., Turner, G. A., Hwang, Y., & Simpson, G. J. (2023) · Analytical Chemistry · doi:10.1021/acs.analchem.3c02638 · Cited by 4
Multiphoton fluorescent images with a spatially varying background signal: a ML deconvolution method
CRIVARO, M., Enjieu-Kadji, H., HATANAKA, R., Nakauchi, S., Bosch, J., Judin, J., RIERA, J., & Kawashima, R. (2010) · Journal of Microscopy · doi:10.1111/j.1365-2818.2010.03473.x · Cited by 4
Effects of pupil filter patterns in line-scan focal modulation microscopy
Shen, S., Pant, S., Chen, R., & Chen, N. (2018) · Journal of Biomedical Optics · doi:10.1117/1.jbo.23.3.036008 · Cited by 4
Photoinduced charge transfer in quasi-one-dimensional polymers in two-photon absorption
Yan, P.-J., Mu, X., Dong, J., & Sun, M. (2020) · RSC Advances · doi:10.1039/d0ra06436j · Cited by 4
Optical Manipulation and Recording of Neural Activity with Wavefront Engineering
Sims, R. R., Bendifallah, I., Blanchard, K., Tanese, D., Emiliani, V., & Papagiakoumou, E. I. (2023) · Neuromethods · doi:10.1007/978-1-0716-2764-8_1 · Cited by 5
Multispot multiphoton Ca2+ imaging in acute myocardial slices
Borile, G., de Mauro, C., Urbani, A., Alfieri, D., Pavone, F. S., & Mongillo, M. (2014) · Journal of Biomedical Optics · doi:10.1117/1.jbo.20.5.051016 · Cited by 4
Applications of Multiphoton Microscopy in Dermatology
So, P. T. C., Yew, E. Y. S., & Rowlands, C. J. (2016) · Elsevier eBooks · doi:10.1016/b978-0-12-802838-4.00019-4 · Cited by 4
Intravital imaging of megakaryocytes
Stegner, D., & Heinze, K. G. (2020) · Platelets · doi:10.1080/09537104.2020.1738366 · Cited by 4
Hybrid Liquid-Crystal/Photonic-Crystal Devices: Current Research and Applications
Hsiao, Y. (2019) · IntechOpen eBooks · doi:10.5772/intechopen.82833 · Cited by 4
Leveraging the periodic interference condition in electro-optic modulators for picosecond pulse generation
Meyer, S., Kutscher, T. F., Lamminger, P., Sommer, F., & Karpf, S. (2023) · Optics Continuum · doi:10.1364/optcon.500969 · Cited by 3
Dual-objective two-photon microscope for volumetric imaging of dense scattering biological samples by bidirectional excitation and collection
Zhai, M., Yu, J., Hu, Y., Yu, H., Xie, B., Yu, Y., Li, D., Wang, A., & Cheng, H. (2024) · Photonics Research · doi:10.1364/prj.516824 · Cited by 3
Photobleaching analysis of fluorescent proteins in two-photon microscopy at high repetition rates
Weng, C. H., & Han, K. Y. (2024) · Optics Communications · doi:10.1016/j.optcom.2024.131059 · Cited by 3
Spectrally and spatially resolved laser‐induced photobleaching of endogenous flavin fluorescence in cardiac myocytes
Chorvatova, A. M., Kirchnerova, J., Cagalinec, M., Mateašík, A., & Chorvat, D., Jr. (2018) · Cytometry Part A · doi:10.1002/cyto.a.23591 · Cited by 3
Restored interlaced volumetric imaging increases image quality and scanning speed during intravital imaging in living mice
Sogabe, M., Ohzeki, M., Fujimoto, K., Sehara‐Fujisawa, A., & Nishimura, S. (2020) · Journal of Biophotonics · doi:10.1002/jbio.201960204 · Cited by 3
Improved image contrast in nonlinear light-sheet fluorescence microscopy using i$$^2$$PIE Pulse compression
Badrodien, I., Neethling, P., & Bosman, G. W. (2024) · Scientific Reports · doi:10.1038/s41598-024-63429-6 · Cited by 3
Gas bubbles induced by combined optical and ultrasound energies for high-resolution deep optical microscopy
Kim, J., Kim, J., Kim, H., & Chang, J. H. (2024) · APL Photonics · doi:10.1063/5.0203205 · Cited by 3
Fast two-photon neuronal imaging and control using a spatial light modulator and ruthenium compounds
Peterka, D. S., Nikolenko, V., Fino, É., Araya, R., Etchenique, R., & Yuste, R. (2010) · Proceedings of SPIE, the International Society for Optical Engineering/Proceedings of SPIE · doi:10.1117/12.842606 · Cited by 3
Calcium Imaging in the Drosophila Olfactory System with a Genetic IndicatorPreprint
Root, C. M., Wong, A. M., Flores, J., & Wang, J. W. (2013) · Cold Spring Harbor Protocols · doi:10.1101/pdb.prot078568 · Cited by 3
Nonlinear excitation fluorescence microscopy: source considerations for biological applications
Wokosin, D. L. (2008) · Proceedings of SPIE, the International Society for Optical Engineering/Proceedings of SPIE · doi:10.1117/12.773610 · Cited by 3
Reduction in DNA Synthesis During Two‐photon Microscopy of Intrinsic Reduced Nicotinamide Adenine Dinucleotide Fluorescence¶
Nichols, M. G., Barth, E. E., & Nichols, J. A. (2005) · Photochemistry and Photobiology · doi:10.1111/j.1751-1097.2005.tb00183.x · Cited by 3
Out-of-equilibrium dynamics of a grid-like Fe( ii ) spin crossover dimer triggered by a two-photon excitation
Velazquez-Garcia, J. D. J., Basuroy, K., Wong, J., Demeshko, S., Meyer, F., Kim, I., Henning, R. W., Staechelin, Y. U., Lange, H., & Techert, S. (2024) · Chemical Science · doi:10.1039/d4sc02933j · Cited by 2
Evaluation of safe exposure time for two-photon microscopy imaging of acrylic-painted mock-ups
Mattana, S., Fovo, A. D., Baria, E., Fontana, R., & Cicchi, R. (2025) · Journal of Physics Photonics · doi:10.1088/2515-7647/ada998 · Cited by 2
Intravital mesoscale optical imaging: challenges, techniques, and future perspectives
Wang, M., Wu, J., & Dai, Q. (2025) · Biophysics Reports · doi:10.52601/bpr.2025.250015 · Cited by 2
Integrative functional optical imaging approaches: Optics, microfluidics, and machine learning for neuroscience in organoids and small-animal models
Wheelock, J. M., Pritchard, R., Kumar, S., & Lu, H. (2025) · Biophysical Journal · doi:10.1016/j.bpj.2025.09.015 · Cited by 2
Simultaneous two- and three-photon multiplane imaging across cortical layers in freely moving mice
Klioutchnikov, A., Wallace, D. J., Berdahl, C., Sugi, A., Sawinski, J., & Kerr, J. N. D. (2026) · Nature Methods · doi:10.1038/s41592-026-03125-7 · Cited by 5
Squeezed light improves sensitivity of microscopy technique
Potma, E. O. (2021) · Nature · doi:10.1038/d41586-021-01514-w · Cited by 2
SADiff: Structure-aware diffusion model for enhancing prostate multiphoton microscopy imaging
Huang, M., Huang, X., Chen, H., Fan, H., Shi, P., & Zhu, X. (2025) · Expert Systems with Applications · doi:10.1016/j.eswa.2025.128447 · Cited by 2
Dispersion control considerations for multiphoton and non-linear laser microscopy applications
Brideau, C., & Stys, P. K. (2009) · Proceedings of SPIE, the International Society for Optical Engineering/Proceedings of SPIE · doi:10.1117/12.809245 · Cited by 2
Intravital Multiphoton Endoscopy
Huland, D. M., Ouzounov, D. G., Rivera, D. R., Brown, C. M., & Xu, C. (2014) · Springer eBooks · doi:10.1007/978-94-017-9361-2_15 · Cited by 2
Is Heralded Two-Photon Excited Fluorescence with Single Absorbers Possible with Current Technology?
Jechow, A. (2022) · Photonics · doi:10.3390/photonics9020052 · Cited by 2
Challenges in mitochondrial profiling during pre-clinical studies
Afonso, G. J. M., Simões, R. F., Pinho, S. L. C., Oliveira, P. J., & Cunha‐Oliveira, T. (2022) · Elsevier eBooks · doi:10.1016/b978-0-323-88462-4.00002-x · Cited by 2
Exposure to fluorescent excitation light induces dose-dependent, irreversible force relaxation in living fibroblast cells
Knoll, S. G. (2016) · Illinois Digital Environment for Access to Learning and Scholarship (University of Illinois at Urbana-Champaign) · Cited by 2
Intracellular molecular diffusion probed with nonlinear optical microscopy
Potma, E. O. (2001) · University of Groningen research database (University of Groningen / Centre for Information Technology) · Cited by 2
Between Life and Death: Reducing Phototoxicity in Super-Resolution MicroscopyPreprint
Tosheva, K. L., Yuan, Y., Pereira, P. M., Culley, S., & Henriques, R. (2019) · Preprints.org · doi:10.20944/preprints201908.0319.v1 · Cited by 2
A Comparison of the Sensitivity of Photodamage Assays in Rat Basophilic Leukemia Cells¶
Barth, E. E., Hallworth, R., & Nichols, M. G. (2005) · Photochemistry and Photobiology · doi:10.1111/j.1751-1097.2005.tb00225.x · Cited by 2
Label-Free Multimodal Multiphoton Intravital Imaging
Park, J., Tu, H., Marjanović, M., & Boppart, S. A. (2021) · Advances in experimental medicine and biology · doi:10.1007/978-981-15-7627-0_7 · Cited by 1
微型双光子显微镜与显微内窥镜方法与应用（特邀）
Kaiyan, 李. L., Yankan, 黄. H., Zixian, 曹. C., Haiyan, 黄. H., & Wenxuan, 梁. L. (2025) · Laser & Optoelectronics Progress · doi:10.3788/lop251757 · Cited by 1
Dynamic glia in the living brain: insights into glial physiology and function from in vivo imaging
Liu, Y., Eyo, U. B., & Lu, T.-Y. (2025) · Journal of Neurophysiology · doi:10.1152/jn.00292.2025 · Cited by 2
Wide-field two-photon random illumination microscopy (2P-RIM)
Benachir, A., Li, X., Fantuzzi, E. M., Giroussens, G., Mangeat, T., Vernuccio, F., Rigneault, H., Sentenac, A., & Heuke, S. (2026) · Science Advances · doi:10.1126/sciadv.aef3487 · Cited by 1
Enabling robust, stable, and accurate nonlinear optical measurements from squeezed light generated in hot rubidium vapor
Allen, C. H., Soucie, M. S., Benson, R., Glezakou-Elbert, O.-M., Jimenez, R., Morrell‐Falvey, J. L., & Ma, Y. (2026) · Quantum Effects and Measurement Techniques in Biology and Biophotonics · doi:10.1117/12.3080097 · Cited by 1
Live-cell STED microscopy enables 50 nm resolution imaging with preserved cell proliferation
Mol, F. N., Dijt, S. J., Burgers, T. C. Q., Maunz, T. M., & Vlijm, R. (2026) · Scientific Reports · doi:10.1038/s41598-026-48958-6 · Cited by 1
Advances in multiphoton microscopy for in vivo skin imaging and dermatologic intervention
Zhao, J., Wu, Z., Mantle, L., & Zeng, H. (2026) · Journal of Innovative Optical Health Sciences · doi:10.1142/s1793545826300144 · Cited by 1
On how to efficiently accelerate brain network analysis on FPGA-based computing system
Gnemmi, G., Crippa, M., Durelli, G. C., Cattaneo, R., Pallotta, G., & Santambrogio, M. D. (2015) · International Conference on ReConFigurable Computing and FPGAs (ReConFig) · doi:10.1109/reconfig.2015.7393330 · Cited by 1
Functional Imaging Using Two-Photon Microscopy in Living Tissue
Vanzetta, I., Deneux, T., Kaszás, A., Katona, G., & Rózsa, B. J. (2012) · Neuromethods · doi:10.1007/978-1-61779-897-9_7 · Cited by 1
Time-Resolved Raman Scattering
van Loosdrecht, D. F. A. P. H. M. (2011) · CRC Press eBooks · doi:10.1201/b11040-20 · Cited by 1
Solvent effect on the femtosecond laser induced two-photon emission from Rhodamine 6G
Pathrose, B. P., Nampoori, V. P. N., Radhakrishnan, P., & Mujeeb, A. (2016) · Journal of Photochemistry and Photobiology A Chemistry · doi:10.1016/j.jphotochem.2016.10.011 · Cited by 1
Neuromodulation Using Optogenetics and Related Technologies
Pedersen, N. P., & Gross, R. E. (2018) · Elsevier eBooks · doi:10.1016/b978-0-12-805353-9.00035-8 · Cited by 2
Joint Optimization of Hadamard Sensing and Reconstruction in Compressed Sensing Fluorescence Microscopy
Wang, A. Q., LaViolette, A. K., Moon, L., Xu, C., & Sabuncu, M. R. (2021) · Lecture notes in computer science · doi:10.1007/978-3-030-87231-1_13 · Cited by 1
Nanoparticle-Assisted Stimulated Emission Depletion (STED) Super-Resolution Nanoscopy
Sivan, Y., & Sonnefraud, Y. (2017) · CRC Press eBooks · doi:10.4324/9781315206530-9 · Cited by 1
Investigations on 2,7-diamino-9-fluorenol photochemistry
Živković, D. T. (2007) · edoc (University of Basel) · doi:10.5451/unibas-004208337 · Cited by 1
Multifocal multiphoton microscopy : new detection methods and biological applications
Martini, J. (2006) · PUB – Publications at Bielefeld University (Bielefeld University) · Cited by 1
Optimizing Spectral Resolution in Supercontinuum-Generation-Based Multimodal fs CARS Microscopy
Slepkov, A. D., Ridsdale, A., Pegoraro, A. F., Labroille, G., & Stolow, A. (2010) · Conference on Lasers and Electro-Optics · doi:10.1364/cleo.2010.cwd2 · Cited by 1
Fluorescent Probes Used for Measuring Intracellular Calcium
Oheim, M. (2015) · Encyclopedia of Life Sciences · doi:10.1002/9780470015902.a0002642.pub2 · Cited by 1
Three-dimensional computation of light scattering by multiple biological cells
Starosta, M. S. (2010) · Texas ScholarWorks (Texas Digital Library) · Cited by 1
Fiber-based Implementations of Coherent anti-Stokes Raman Scattering Microspectroscopy and Microscopy
Andresen, E. R. (2007) · Cited by 1
Engineering a Photo-Control Mechanism for RNA Interference
Blidner, R. (2007) · doi:10.31390/gradschool_dissertations.1331 · Cited by 1
Application of femtosecond lasers for subcellular nanosurgery
Maxwell, I. Z. (2007) · PhDT · Cited by 1
Multimodal measurements of phototoxicity in nonlinear optical microscopyPreprint
Zhang, X., Dorlhiac, G., Landry, M. P., & Streets, A. (2021) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2021.08.27.457929 · Cited by 1
References
(2005) · Springer series in chemical physics · doi:10.1007/3-540-28882-1_9 · Cited by 1
Examination of Diagnostic Features in Multiphoton Microscopy and Optical Coherence Tomography Images of Ovarian Tumorigenesis in a Mouse Model
Watson, J. M. (2013) · UA Campus Repository (The University of Arizona)
Probing vesicle dynamics within small synapses
Lemke, E. A. (2006) · MPG.PuRe (Max Planck Society) · doi:10.53846/goediss-2152
Coherent Raman Scattering: Applications in Imaging and Sensing.
Cui, M. (2009)
Generation of Novel Photochromic GFPs: Fluorescent Probes for RESOLFT-type Microscopy at Low Light Intensities
Grotjohann, T. (2012) · doi:10.53846/goediss-1490
Visualization of autoimmune processes in chronic neuroinflammation by means of two-photon laser scanning microscopy
Herz, J. (2010) · Universitätsbibliothek der FU Berlin Hochschulschriftenstelle u. Dokumentenserver · doi:10.17169/refubium-6930
Intravital Multiphoton Microscopy Analysis of Spatial Relationships in Murine Skull Bone Marrow
Engelke, K. (2009) · Elektronische Hochschulschriften der LMU München (Ludwig-Maximilians-Universität München) · doi:10.5282/edoc.10587
A Dithienylethene that gates a spontaneous reaction and photolyzes with visible light and synthetic progress towards a corresponding hexatriene
Warford, C. C. (2012) · Summit (Simon Fraser University)

Authors: Hopt, A., & Neher, E.
Affiliations: Max Planck Institute for Biophysical Chemistry (DE)
Max Planck Society (DE)
Corresponding author: Neher, Erwin
ORCID: Neher, Erwin
Venue: Biophysical Journal
Publisher: Elsevier BV
Published: 2001-04
Volume: 80
Issue: 4
Pages: 2029-2036
ISSN: 0006-3495 (ISSN-L)
DOI: 10.1016/s0006-3495(01)76173-5
PMC: PMC1301392
OpenAlex: W2089975180
Cited by: 442
References: 27
Citations by year: 2026: 15 · 2025: 15 · 2024: 15 · 2023: 16 · 2022: 23 · 2021: 21 · 2020: 16 · 2019: 14 · 2018: 14 · 2017: 18 · 2016: 16 · 2015: 18 · 2014: 24 · 2013: 18 · 2012: 13
FWCI: 9.13
Type: journal article
Language: en
Open access: bronze
doi.org
Licence: www.elsevier.com/tdm/userlicense/1.0/ (tdm)
www.elsevier.com/open-access/userlicense/1.0/ (vor, 4489 d)
Locations: Biophysical Journal (publishedVersion)
PubMed Central (publishedVersion)
PubMed (publishedVersion)
Max Planck Institute for Plasma Physics (submittedVersion)
MPG.PuRe (Max Planck Society) (submittedVersion)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Advanced Fluorescence Microscopy Techniques
Physical Sciences › Materials Science › Electronic, Optical and Magnetic Materials › Nonlinear Optical Materials Studies
Physical Sciences › Physics and Astronomy › Atomic and Molecular Physics, and Optics › Laser-Matter Interactions and Applications
Keywords: two-photon fluorescence microscopy, photodamage, laser pulse shape, focal volume, excitation intensity, adrenal chromaffin cells, calcium imaging, degranulation
MeSH terms: Adrenal Glands, Animals, Calcium, Cattle, Lasers, Light, Microscopy, Fluorescence, Models, Theoretical, Time Factors, Models, Statistical, Photons, Chromaffin Cells

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

Highly Nonlinear Photodamage in Two-Photon Fluorescence Microscopy

Abstract

Overview

Integrity signals

Verification

Claims the paper makes

Claims other papers make about this one

Citations

Cites

Cited by

Related

Metadata

Record

About these checks