Student or educator? Sign up with your school email and get 6 months of Pro free.
CoChat
Sign Up
Cited by 349

The DOI matches its Crossref record; 24 of the 24 references it cites were found in a registry or an index.

Myoplasmic binding of fura-2 investigated by steady-state fluorescence and absorbance measurements

Open access
Konishi, M., Olson, A., Hollingworth, S., Baylor, S.M.·1988·Biophysical Journal
DOI 10.1016/s0006-3495(88)83045-5
Open this paper in CoChat — free account

Abstract

Binding of the fluorescent Ca2+ indicator dye fura-2 by intracellular constituents has been investigated by steady-state optical measurements. Fura-2's (a) fluorescence intensity, (b) fluorescence emission anisotropy, (c) fluorescence emission spectrum, and (d) absorbance spectra were measured in glass capillary tubes containing solutions of purified myoplasmic proteins; properties b and c were also measured in frog skeletal muscle fibers microinjected with fura-2. The results indicate that more than half, and possibly as much as 85%, of fura-2 molecules in myoplasm are in a protein-bound form, and that the binding changes many properties of the dye. For example, in vitro characterization of the Ca2+-dye reaction indicates that when fura-2 is bound to aldolase (a large and abundant myoplasmic protein), the dissociation constant of the dye for Ca2+ is three- to fourfold larger than that measured in the absence of protein. The problems raised by intracellular binding of fura-2 to cytoplasmic proteins may well apply to cells other than skeletal muscle fibers.

Overview

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.1016/s0006-3495(88)83045-5

Cites

24 of 24 references verified0 citation errors · 0 not checkable

Statistics

Verification

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Citations

Cites

Show all 24 references

Cited by

See all 349

Metadata

Authors
Konishi, M., Olson, A., Hollingworth, S., & Baylor, S. M.
Venue
Biophysical Journal
Publisher
Elsevier BV
Published
1988-12
Volume
54
Issue
6
Pages
1089-1104
ISSN
0006-3495 (ISSN-L)
DOI
10.1016/s0006-3495(88)83045-5
OpenAlex
W1999132987
Cited by
349
References
24
FWCI
27.38
Type
journal article
Language
en
Open access
hybrid

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

How to cite

Cite CoChat for the checks on this page, and the paper for its own findings.

This page
CoChat. (2026). Citation check: Myoplasmic binding of fura-2 investigated by steady-state fluorescence and absorbance measurements. https://app.cochat.ai/papers/10.1016/s0006-3495(88)83045-5
The paper
Konishi, M., Olson, A., Hollingworth, S., & Baylor, S. M. (1988). Myoplasmic binding of fura-2 investigated by steady-state fluorescence and absorbance measurements. Biophysical Journal. https://doi.org/10.1016/s0006-3495(88)83045-5

Data sources

Bibliographic data: Semantic Scholar, OpenAlex, Crossref.

Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.