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Calcium Rises Abruptly and Briefly Throughout the Cell at the Onset of Anaphase
Abstract
Continuous measurement and imaging of the intracellular free calcium ion concentration ([Ca 2+ ] i ) of mitotic and interphase PtK 1 cells was accomplished with the new fluorescent Ca 2+ indicator fura-2. No statistically significant difference between basal [Ca 2+ ] i of interphase and mitotic cells was detected. However, mitotic cells showed a rapid elevation of [Ca 2+ ] i from basal levels of 130 n M to 500 to 800 n M at the metaphase-anaphase transition. The [Ca 2+ ] i transient was brief, lasting approximately 20 seconds and the elevated [Ca 2+ ] i appeared uniformly distributed over the entire spindle and central region of the cell. The close temporal association of the [Ca 2+ ] i transient with the onset of anaphase suggests that calcium may have a signaling role in this event.
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Citations
Cited by
Feedback control of mitosis in budding yeast
Li, R., & Murray, A. W. (1991) · Cell · doi:10.1016/0092-8674(81)90015-5 · Cited by 1,174
Fluorescence ratio imaging: a new window into intracellular ionic signaling
Tsien, R. Y., & Poenie, M. (1986) · Trends in Biochemical Sciences · doi:10.1016/0968-0004(86)90245-8 · Cited by 414
Self-citationSpatially Resolved Calcium Dynamics of Mammalian Purkinje Cells in Cerebellar Slice
Tank, D. W., Sugimori, M., Connor, J. A., & Llinás, R. R. (1988) · Science · doi:10.1126/science.2847315 · Cited by 406
Myoplasmic binding of fura-2 investigated by steady-state fluorescence and absorbance measurements
Konishi, M., Olson, A., Hollingworth, S., & Baylor, S. M. (1988) · Biophysical Journal · doi:10.1016/s0006-3495(88)83045-5 · Cited by 349
Alteration of intracellular Fura-2 fluorescence by viscosity: A simple correction
Poenie, M. (1990) · Cell Calcium · doi:10.1016/0143-4160(90)90062-y · Cited by 309
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Metadata
- Authors
- Poenie, M., Alderton, J., Steinhardt, R., & Tsien, R.
- Venue
- Science
- Published
- 1986-08-22
- Volume
- 233
- Issue
- 4766
- Pages
- 886-889
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2088233139
- Cited by
- 539
- References
- 0
- FWCI
- 27.65
- Type
- journal article
- Language
- en
- Open access
- closed
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- Poenie, M., Alderton, J., Steinhardt, R., & Tsien, R. (1986). Calcium Rises Abruptly and Briefly Throughout the Cell at the Onset of Anaphase. Science. https://doi.org/10.1126/science.3755550
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