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Cited by 573Diffusion Coefficients in LiquidsHydrogels: synthesis, properties, applicationsCellular Mechanics and Interactions

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Diffusion of Macromolecules in Agarose Gels: Comparison of Linear and Globular Configurations

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Pluen, Alain, Netti, Paolo A., Jain, Rakesh K., Berk, David A.·1999·Biophysical Journal
DOI 10.1016/s0006-3495(99)76911-0PMC PMC1300351
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Abstract

The diffusion coefficients (D) of different types of macromolecules (proteins, dextrans, polymer beads, and DNA) were measured by fluorescence recovery after photobleaching (FRAP) both in solution and in 2% agarose gels to compare transport properties of these macromolecules. Diffusion measurements were conducted with concentrations low enough to avoid macromolecular interactions. For gel measurements, diffusion data were fitted according to different theories: polymer chains and spherical macromolecules were analyzed separately. As chain length increases, diffusion coefficients of DNA show a clear shift from a Rouse-like behavior (DG congruent with N0-0.5) to a reptational behavior (DG congruent with N0-2.0). The pore size, a, of a 2% agarose gel cast in a 0.1 M PBS solution was estimated. Diffusion coefficients of the proteins and the polymer beads were analyzed with the Ogston model and the effective medium model permitting the estimation of an agarose gel fiber radius and hydraulic permeability of the gels. Not only did flexible macromolecules exhibit greater mobility in the gel than did comparable-size rigid spherical particles, they also proved to be a more useful probe of available space between fibers.

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  • The measured diffusion coefficients were in good agreement with literature values in aqueous solutions and their mass dependence.

    “Using this method, our measured diffusion coefficients were in good agreement with literature values in aqueous solutions23,24 and their mass dependence (inset to Fig. 1b).”

    From Brain clearance is reduced during sleep and anesthesia (2024) · cites this paper as [24]

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Metadata

Authors
Pluen, A., Netti, P. A., Jain, R. K., & Berk, D. A.
Affiliations
Harvard University (US)
Corresponding author
Jain, Rakesh K.
ORCID
Venue
Biophysical Journal
Publisher
Elsevier BV
Published
1999-07
Volume
77
Issue
1
Pages
542-552
ISSN
0006-3495 (ISSN-L)
DOI
10.1016/s0006-3495(99)76911-0
PMC
PMC1300351
OpenAlex
W2130420151
Cited by
573
References
2
Citations by year
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FWCI
5.54
Type
journal article
Language
en
Open access
Licence
Locations
  • Biophysical Journal (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
  • Research Explorer (The University of Manchester) (submittedVersion)
Topics
  • Physical Sciences › Chemistry › Physical and Theoretical Chemistry › Diffusion Coefficients in Liquids
  • Physical Sciences › Materials Science › Biomaterials › Hydrogels: synthesis, properties, applications
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
Keywords
macromolecular diffusion, agarose gel, diffusion coefficient, DNA diffusion, reptation, polymer chain length, effective medium model, hydraulic permeability, pore size, protein diffusion, dextran, polymer beads, gel porosity
MeSH terms
Animals, Cattle, Chickens, Dextrans, Diffusion, DNA, Gels, Particle Size, Polymers, Proteins, Sepharose, Porosity, Base Pairing

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