Ascorbate inhibits iNOS expression in endotoxin‐ and IFNγ‐stimulated rat skeletal muscle endothelial cells
Open accessAbstract
Lipopolysaccharide endotoxin and interferon-gamma induced inducible nitric oxide synthase (iNOS) protein expression and nitrite/nitrate formation in microvascular endothelial cell cultures (ECs) derived from rat skeletal muscle. Pretreatment of ECs with ascorbate accumulated a large amount of ascorbate inside the cells and consequently decreased both intracellular oxidant level and iNOS induction. These effects of ascorbate were abolished in the presence of exogenous superoxide generated by xanthine oxidase/xanthine plus catalase but were not altered when N-nitro-L-arginine methyl ester was applied to inhibit nitric oxide synthesis. Ascorbate also attenuated the activation of transcription factor IRF-1 but not NF kappa B. These results indicate that ascorbate inhibits iNOS expression in ECs by an antioxidant mechanism independent of both NF kappa B activation and the reported negative feedback effect of nitric oxide.
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- Authors
- Wu, F., Tyml, K., & Wilson, J. X.
- Affiliations
- Western University (CA)
- Corresponding author
- Wilson, John X.
- ORCID
- Wu, Feng
- Venue
- FEBS Letters
- Publisher
- Wiley
- Published
- 2002-05-14
- Volume
- 520
- Issue
- 1-3
- Pages
- 122-126
- ISSN
- 0014-5793 (ISSN-L)
- 1873-3468
- OpenAlex
- W2063749178
- Cited by
- 67
- References
- 27
- Citations by year
- 2025: 2 · 2023: 4 · 2022: 2 · 2021: 3 · 2020: 1 · 2019: 1 · 2018: 1 · 2017: 1 · 2015: 1 · 2014: 4 · 2013: 2
- FWCI
- 4.80
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- FEBS Letters (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Physiology › Nitric Oxide and Endothelin Effects
- Health Sciences › Nursing › Nutrition and Dietetics › Vitamin C and Antioxidants Research
- Health Sciences › Medicine › Biochemistry › Antioxidant Activity and Oxidative Stress
- Keywords
- vitamin C, inducible nitric oxide synthase, iNOS expression, endotoxin, lipopolysaccharide, interferon-gamma, nitric oxide, superoxide, antioxidant defense, IRF1, NF-κB
- MeSH terms
- Animals, Ascorbic Acid, Cells, Cultured, DNA-Binding Proteins, Endothelium, Vascular, Enzyme Induction, Interferon-gamma, Lipopolysaccharides, Male, Nitric Oxide, Phosphoproteins, NF-kappa B, Oxidants, Rats, Wistar, Oxidative Stress, Muscle, Skeletal, Nitric Oxide Synthase, Interferon Regulatory Factor-1, Rats, Nitric Oxide Synthase Type II
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
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