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Mitochondrial membrane potential and hydroethidine‐monitored superoxide generation in cultured cerebellar granule cells
Open accessAbstract
Mitochondrial depolarisation has been reported to enhance the generation of superoxide anion (O⋅− 2) in a number of cell preparations while an inhibition has been observed with isolated mitochondria. Cerebellar granule cells equilibrated with >1 μM hydroethidine (dihydroethidium) which is oxidised to the fluorescent ethidium cation by O⋅− 2 showed a large increase in fluorescence on protonophore addition. However, controls showed the fluorescent enhancement to be a consequence of release of unbound preformed ethidium from the mitochondrial matrix within the cell with resultant fluorescent enhancement. This ambiguity was removed by the use of low (1 μM) concentrations of dye in which case generated ethidium remained bound within the mitochondria. Under these conditions antimycin A, but not protonophore addition, produced an increase in fluorescence. It is concluded that excess ethidium acts as an indicator of mitochondrial membrane potential obscuring the monitoring of O⋅− 2 and that certain experiments employing this indicator in cells may require re‐evaluation.
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Skulachev, V. P. (1996) · Quarterly Reviews of Biophysics · doi:10.1017/s0033583500005795 · Cited by 703
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Metadata
- Authors
- Budd, S. L., Castilho, R. F., & Nicholls, D. G.
- Venue
- FEBS Letters
- Publisher
- Wiley
- Published
- 1997-09-22
- Volume
- 415
- Issue
- 1
- Pages
- 21-24
- ISSN
- 0014-5793 (ISSN-L)
- 1873-3468
- OpenAlex
- W1981783025
- Cited by
- 222
- References
- 1
- FWCI
- 3.26
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Budd, S. L., Castilho, R. F., & Nicholls, D. G. (1997). Mitochondrial membrane potential and hydroethidine‐monitored superoxide generation in cultured cerebellar granule cells. FEBS Letters. https://doi.org/10.1016/s0014-5793(97)01088-0
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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