d ‐Aspartate stimulation of testosterone synthesis in rat Leydig cells
Abstract
D-Aspartate increases human chorionic gonadotropin-induced testosterone production in purified rat Leydig cells. L-Aspartate, D-,L-glutamate or D-,L-asparagine could not substitute for D-aspartate and this effect was independent of glutamate receptor activation. Testosterone production was enhanced only in cells cultured with D-aspartate for more than 3 h. The increased production of testosterone was well correlated with the amounts of D-aspartate incorporated into the Leydig cells, and L-cysteine sulfinic acid, an inhibitor of D-aspartate uptake, suppressed both testosterone production and intracellular D-aspartate levels. D-Aspartate therefore is presumably taken up into cells to increase steroidogenesis. Intracellular D-aspartate probably acts on cholesterol translocation into the inner mitochondrial membrane, the rate-limiting process in steroidogenesis.
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Associations between D-aspartic acid and testosterone or luteinizing hormone are largely based on animal studies.
“Nonetheless, these associations are largely based on animal studies.[Citation23–25]”
From Ergogenic properties, safety evaluation and regulations of selected ingredients in testosterone booster dietary supplements (2024) · cites this paper as [25]
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- Authors
- Nagata, Y., Homma, H., Lee, J.-A., & Imai, K.
- Affiliations
- The University of Tokyo (JP)
- Venue
- FEBS Letters
- Publisher
- Wiley
- Published
- 1999-02-12
- Volume
- 444
- Issue
- 2-3
- Pages
- 160-164
- ISSN
- 0014-5793 (ISSN-L)
- 1873-3468
- OpenAlex
- W2079700548
- Cited by
- 185
- References
- 40
- Citations by year
- 2026: 3 · 2025: 7 · 2024: 12 · 2023: 7 · 2022: 5 · 2021: 4 · 2020: 6 · 2019: 11 · 2018: 2 · 2017: 10 · 2016: 4 · 2015: 9 · 2014: 5 · 2013: 5 · 2012: 6
- FWCI
- 6.87
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- FEBS Letters (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Reproductive Medicine › Sperm and Testicular Function
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Hormonal and reproductive studies
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Amino Acid Enzymes and Metabolism
- Keywords
- D-aspartate, testosterone production, Leydig cells, steroidogenesis, human chorionic gonadotropin, D-aspartate uptake, cholesterol translocation, inner mitochondrial membrane
- MeSH terms
- Animals, Aspartic Acid, Cholesterol, Cysteine, Bucladesine, Chorionic Gonadotropin, Leydig Cells, Male, Testis, Testosterone, Rats, Sprague-Dawley, Neurotransmitter Agents, Rats
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