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Real‐time observation of acrosomal dispersal from mouse sperm using GFP as a marker protein
Abstract
We produced transgenic mouse lines that accumulate mutated green fluorescent protein (EGFP) in sperm acrosome, a membrane limited organelle overlying the nucleus. The sperm showed normal fertilizing ability and the integrity of their acrosome was easily examined in a non‐invasive manner by tracing the GFP in individual ‘live’ sperm with fluorescent microscopy. The time required for the dispersal of acrosomal contents was demonstrated to be approximately 3 s after the onset of acrosome reaction.
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Cited by
In vitro production of functional sperm in cultured neonatal mouse testes
Sato, T., Katagiri, K., Gohbara, A., Inoue, K., Ogonuki, N., Ogura, A., Kubota, Y., & Ogawa, T. (2011) · Nature · doi:10.1038/nature09850 · Cited by 746
In vitro production of fertile sperm from murine spermatogonial stem cell lines
Sato, T., Katagiri, K., Yokonishi, T., Kubota, Y., Inoue, K., Ogonuki, N., Matoba, S., Ogura, A., & Ogawa, T. (2011) · Nature Communications · doi:10.1038/ncomms1478 · Cited by 228
Sperm-borne phospholipase C zeta-1 ensures monospermic fertilization in mice
Nozawa, K., Satouh, Y., Fujimoto, T., Oji, A., & Ikawa, M. (2018) · Scientific Reports · doi:10.1038/s41598-018-19497-6 · Cited by 140
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Metadata
- Authors
- Nakanishi, T., Ikawa, M., Yamada, S., Parvinen, M., Baba, T., Nishimune, Y., & Okabe, M.
- Venue
- FEBS Letters
- Publisher
- Wiley
- Published
- 1999-04-23
- Volume
- 449
- Issue
- 2-3
- Pages
- 277-283
- ISSN
- 0014-5793 (ISSN-L)
- 1873-3468
- OpenAlex
- W2014014112
- Cited by
- 201
- References
- 0
- FWCI
- 6.21
- Type
- journal article
- Language
- en
- Open access
- closed
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- Nakanishi, T., Ikawa, M., Yamada, S., Parvinen, M., Baba, T., Nishimune, Y., & Okabe, M. (1999). Real‐time observation of acrosomal dispersal from mouse sperm using GFP as a marker protein. FEBS Letters. https://doi.org/10.1016/s0014-5793(99)00433-0
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