In vitro production of fertile sperm from murine spermatogonial stem cell lines
Open accessAbstract
Spermatogonial stem cells (SSCs) are the only stem cells in the body that transmit genetic information to the next generation. The long-term propagation of rodent SSCs is now possible in vitro, and their genetic modification is feasible. However, their differentiation into sperm is possible only under in vivo conditions. Here we show a new in vitro system that can induce full spermatogenesis from SSC lines or any isolated SSCs. The method depends on an organ culture system onto which SSCs are transplanted. The settled SSCs form colonies and differentiate up into sperm. The resultant haploid cells are fertile, and give rise to healthy offspring through micro-insemination. In addition, the system can induce spermatogenesis from SSCs that show spermatogenic failure due to a micro-environmental defect in their original testes. Thus, an in vitro system is established that can be used to correct or manipulate the micro-environmental conditions required for proper spermatogenesis from murine SSC lines.
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Niche aging is a major contributor to the functional decline of GSCs.
“Moreover, consecutive transplantation of spermatogonial stem cells to the testes of young male mice was sufficient to maintain stem cell function for extended periods of time, underscoring a major role of aging of the niche in GSC functional decline and suggesting the potential for reversing aging phenotypes by targeting the niche155.”
From Stem cell aging: mechanisms, regulators and therapeutic opportunities (2014) · cites this paper as [155]
Repeatedly transplanting spermatogonial stem cells into young mouse testes kept stem cell function going for long periods.
“Moreover, consecutive transplantation of spermatogonial stem cells to the testes of young male mice was sufficient to maintain stem cell function for extended periods of time, underscoring a major role of aging of the niche in GSC functional decline and suggesting the potential for reversing aging phenotypes by targeting the niche155.”
From Stem cell aging: mechanisms, regulators and therapeutic opportunities (2014) · cites this paper as [155]
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- Authors
- Sato, T., Katagiri, K., Yokonishi, T., Kubota, Y., Inoue, K., Ogonuki, N., Matoba, S., Ogura, A., & Ogawa, T.
- Affiliations
- Yokohama City University (JP)
- RIKEN BioResource Research Center (JP)
- Advanced Medical Research Center, Yokohama City University (JP)
- Corresponding author
- Ogawa, Takehiko
- Venue
- Nature Communications
- Published
- 2011-09-13
- Volume
- 2
- Issue
- 1
- Pages
- 472-472
- ISSN
- 2041-1723 (ISSN-L)
- OpenAlex
- W1986307220
- Cited by
- 228
- References
- 37
- Citations by year
- 2026: 8 · 2025: 6 · 2024: 10 · 2023: 17 · 2022: 21 · 2021: 21 · 2020: 19 · 2019: 16 · 2018: 21 · 2017: 18 · 2016: 18 · 2015: 16 · 2014: 10 · 2013: 14 · 2012: 13
- FWCI
- 13.27
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Nature Communications (publishedVersion)
- PubMed (publishedVersion)
- RePEc: Research Papers in Economics (submittedVersion)
- Funders
- Japan Society for the Promotion of Science (23500507, 21592080, 22800084)
- Topics
- Health Sciences › Medicine › Reproductive Medicine › Sperm and Testicular Function
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Animal Genetics and Reproduction
- Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Reproductive Biology and Fertility
- Keywords
- spermatogonial stem cells, in vitro spermatogenesis, organ culture, fertility, microinsemination, spermatogenic failure, microenvironment, germ cell differentiation, mice
- MeSH terms
- Animals, Cell Differentiation, Male, Mice, Transgenic, Spermatogenesis, Spermatogonia, Stem Cells, Mice, In Vitro Techniques
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