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Cited by 259Sperm and Testicular FunctionUrologic and reproductive health conditionsMale Reproductive Health Studies

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Leukocytospermia is associated with increased reactive oxygen species production by human spermatozoa

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Saleh, Ramadan A, Agarwal, Ashok, Kandirali, Engin, Sharma, Rakesh K, Thomas, Anthony J, Nada, Essam A, Evenson, Donald P, Alvarez, Juan G·2002·Fertility and Sterility
DOI 10.1016/s0015-0282(02)04237-1
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Abstract

To investigate the role of increased seminal leukocytes in enhancing reactive oxygen species (ROS) production by human spermatozoa.A prospective study. Male infertility clinic.Forty-eight infertile men. Standard semen analysis. Assessment of sperm nuclear DNA damage by sperm chromatin structure assay. Incubation of spermatozoa from nonleukocytospermic samples with blood neutrophils. Spontaneous and phorbol 12-myristate 13-acetate (PMA)-induced ROS production in pure-sperm suspensions (after removal of leukocytes) as measured by a chemiluminescence assay. Levels of spontaneous and PMA-induced ROS production in pure-sperm suspensions from the infertile men with a diagnosis of leukocytospermia (n = 16) were significantly higher compared with the case of infertile men without leukocytospermia (n = 32) and with the case of a control group of healthy volunteers (n = 13). A similar pattern of increased ROS was observed when spermatozoa were incubated with blood neutrophils. Leukocytospermia was associated with a significant decrease in sperm motility and increase in DNA damage. Increased seminal leukocytes may play a role in stimulating ROS production by human spermatozoa. Such stimulation may be mediated via direct cell-cell contact or by soluble products released by leukocytes. Poor sperm quality in leukocytospermic samples may be due to leukocyte-mediated oxidative stress.

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Metadata

Authors
Saleh, R. A., Agarwal, A., Kandirali, E., Sharma, R. K., Thomas, A. J., Nada, E. A., Evenson, D. P., & Alvarez, J. G.
Affiliations
  • Cleveland Clinic (US)
  • South Valley University (EG)
  • South Dakota State University (US)
Corresponding author
Agarwal, Ashok
ORCID
Venue
Fertility and Sterility
Publisher
Elsevier BV
Published
2002-12
Volume
78
Issue
6
Pages
1215-1224
ISSN
0015-0282 (ISSN-L)
DOI
10.1016/s0015-0282(02)04237-1
OpenAlex
W2129317574
Cited by
259
References
0
Citations by year
2026: 6 · 2025: 5 · 2024: 6 · 2023: 11 · 2022: 9 · 2021: 13 · 2020: 11 · 2019: 5 · 2018: 11 · 2017: 14 · 2016: 10 · 2015: 13 · 2014: 21 · 2013: 11 · 2012: 16
FWCI
7.45
Type
journal article
Language
en
Open access
Licence
Locations
  • Fertility and Sterility (publishedVersion)
  • PubMed (publishedVersion)
Funders
Cleveland Clinic Foundation
Topics
  • Health Sciences › Medicine › Reproductive Medicine › Sperm and Testicular Function
  • Health Sciences › Medicine › Urology › Urologic and reproductive health conditions
  • Health Sciences › Medicine › Urology › Male Reproductive Health Studies
Keywords
leukocytospermia, reactive oxygen species, spermatozoa, seminal leukocytes, neutrophils, oxidative stress, male infertility, sperm DNA damage, sperm motility, phorbol myristate acetate
MeSH terms
DNA Damage, Humans, Infertility, Male, Leukocytes, Male, Neutrophils, Prospective Studies, Reference Values, Sperm Motility, Spermatozoa, Tetradecanoylphorbol Acetate, Reactive Oxygen Species

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