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Cited by 169

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Sequence specificity of mRNA N6-adenosine methyltransferase

Csepany, T, Lin, A, Baldick, C J, Beemon, K·1990·Journal of Biological Chemistry
DOI 10.1016/s0021-9258(17)30477-5
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FIG. 1 Analysis of m6Ap at site 8485 in wild-type RSV and mutant 8484A. The presence of m6Ap in a 246-nucleotide fragment containing a single m6A site at nucleotide 8485 was investigated by the in vitro labeling method described under "Experimental Procedures." This involved two-dimensional thin-layer chromatography. The first dimension is from bottom to top, and the second is from left to right. A, wild-type virus; B, mutant 8484A.
FIG. 1 Analysis of m6Ap at site 8485 in wild-type RSV and mutant 8484A. The presence of m6Ap in a 246-nucleotide fragment containing a single m6A site at nucleotide 8485 was investigated by the in vitro labeling method described under "Experimental Procedures." This involved two-dimensional thin-layer chromatography. The first dimension is from bottom to top, and the second is from left to right. A, wild-type virus; B, mutant 8484A.CC-BY · From the PDF
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FIG. 2 RSV RNAs in chicken cells infected with wild-type (WT) or mutant viruses. The levels of unspliced, env, and src mRNAs were determined by an RNase protection assay as described under "Experimental Procedures." Probes spanning the env and src splice acceptor sites were used simultaneously in this assay. M represents RNA markers. The four-site mutants 8487G and 8484U also contained mutations 8016G and 7415U/7425U.
FIG. 2 RSV RNAs in chicken cells infected with wild-type (WT) or mutant viruses. The levels of unspliced, env, and src mRNAs were determined by an RNase protection assay as described under "Experimental Procedures." Probes spanning the env and src splice acceptor sites were used simultaneously in this assay. M represents RNA markers. The four-site mutants 8487G and 8484U also contained mutations 8016G and 7415U/7425U.CC-BY · From the PDF

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Cited by

Metadata

Authors
Csepany, T., Lin, A., Baldick, C. J., & Beemon, K.
Venue
Journal of Biological Chemistry
Publisher
Elsevier BV
Published
1990-11
Volume
265
Issue
33
Pages
20117-20122
ISSN
0021-9258
DOI
10.1016/s0021-9258(17)30477-5
Cited by
169
References
0
Language
en
Licence

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