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Actin-based cytoskeleton regulates a chloride channel and cell volume in a renal cortical collecting duct cell line.
Open accessAbstract
The regulatory volume decrease (RVD) of a renal cortical collecting duct cell line (RCCT-28A) exposed to a hypotonic solution was studied using electronic cell sizing to measure cell volume and the patch clamp technique to measure Cl- channel activity. Results demonstrate that RVD was mediated in part by KCl loss through separate K+ and Cl- channels. The Cl- channel had a conductance of 305 pS and was activated by cell swelling, membrane stretch, and disruption of F-actin by dihydrocytochalasins. In contrast, stabilizing F-actin with phalloidin prevented swelling and stretch activation of the Cl- channel and inhibited the RVD. Thus, the state of actin polymerization regulates the probability of the 305 pS Cl- channel being open. Short actin filaments activate whereas long actin filaments inactivate the channel. Taken together, our studies suggest that RVD in this renal collecting duct cell line cell is mediated in part by a 305 pS Cl- channel, which is activated, during cell swelling, by a signaling pathway that includes disruption of F-actin.
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Cited by
Functional significance of cell volume regulatory mechanisms
LANG, F., BUSCH, G. L., RITTER, M., VÖLKL, H., WALDEGGER, S., GULBINS, E., & HÄUSSINGER, D. (1998) · Physiological Reviews · doi:10.1152/physrev.1998.78.1.247 · Cited by 1,891
Physiology of cell volume regulation in vertebrates
Hoffmann, E. K., Lambert, I. H., & Pedersen, S. F. (2009) · Physiological Reviews · doi:10.1152/physrev.00037.2007 · Cited by 1,441
Molecular Structure and Physiological Function of Chloride Channels
Jentsch, T. J., Stein, V., Weinreich, F., & Zdebik, A. A. (2002) · Physiological Reviews · doi:10.1152/physrev.00029.2001 · Cited by 1,256
Metadata
- Authors
- Schwiebert, E. M., Mills, J. W., & Stanton, B. A.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1994-03
- Volume
- 269
- Issue
- 10
- Pages
- 7081-7089
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W1531796828
- Cited by
- 200
- References
- 0
- FWCI
- 6.25
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Schwiebert, E. M., Mills, J. W., & Stanton, B. A. (1994). Actin-based cytoskeleton regulates a chloride channel and cell volume in a renal cortical collecting duct cell line. Journal of Biological Chemistry. https://doi.org/10.1016/s0021-9258(17)37249-6
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