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Isolation of a lytic, pore-forming protein (perforin) from cytolytic T-lymphocytes.
Open accessAbstract
Cytolytic granules from a T-cell line with specific cytolytic activity were isolated. Granules were solubilized and fractionated on a TSK 4000 gel filtration column. Lytic activity was eluted as a single retarded peak. Polyacrylamide-gel electrophoresis in the presence of sodium dodecyl sulfate indicated that the lytic fractions contained a single protein (perforin) with an apparent molecular weight of approximately 66 kDa. It separated well from the other proteins present in the granules. Isolated perforin polymerized and inserted into lipid bilayers in the presence of Ca2+, forming tubular structures with inner diameters varying from 6 to 16 nm. Lipid insertion of perforin was demonstrated using a membrane-restricted, photoactivatable probe. The lytic properties of perforin suggest an important role of this particular protein during cytolytic T-cell-mediated target cell lysis.
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Cited by
Perforin-mediated target-cell death and immune homeostasis
Voskoboinik, I., Smyth, M. J., & Trapani, J. A. (2006) · Nature reviews. Immunology · doi:10.1038/nri1983 · Cited by 592
Jenne, D. E., & Tschopp, J. (1988) · Immunological Reviews · doi:10.1111/j.1600-065x.1988.tb00749.x · Cited by 257
Self-citationCytotoxic CD4 T Cells—Friend or Foe during Viral Infection?
Juno, J. A., van Bockel, D., Kent, S. J., Kelleher, A. D., Zaunders, J. J., & Munier, C. M. L. (2017) · Frontiers in Immunology · doi:10.3389/fimmu.2017.00019 · Cited by 195
Ritter, A. T., Kapnick, S. M., Murugesan, S., Schwartzberg, P. L., Griffiths, G. M., & Lippincott-Schwartz, J. (2017) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1710751114 · Cited by 104
Breaching the Bacterial Envelope: The Pivotal Role of Perforin-2 (MPEG1) Within Phagocytes
Merselis, L. C., Rivas, Z. P., & Munson, G. P. (2021) · Frontiers in Immunology · doi:10.3389/fimmu.2021.597951 · Cited by 18
Metadata
- Authors
- Masson, D., & Tschopp, J.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1985-08
- Volume
- 260
- Issue
- 16
- Pages
- 9069-9072
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W1591446606
- Cited by
- 292
- References
- 0
- FWCI
- 12.59
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Masson, D., & Tschopp, J. (1985). Isolation of a lytic, pore-forming protein (perforin) from cytolytic T-lymphocytes. Journal of Biological Chemistry. https://doi.org/10.1016/s0021-9258(17)39328-6
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