The DOI matches its Crossref record; 27 of the 27 references it cites were found in a registry or an index.
Complete sequence of the staphylococcal gene encoding protein A. A gene evolved through multiple duplications.
Open accessAbstract
The gene coding for protein A from Staphylococcus aureus has been isolated by molecular cloning, and a subclone containing an 1.8-kilobase insert was found to give a functional protein A in Escherichia coli. The complete nucleotide sequence of the insert, including the structural gene and the 5' and 3' flanking sequences, has been determined. Starting from a TTG initiator codon, an open reading frame comprising 1527 nucleotides gives a preprotein of 509 amino acids and a predicted Mr = 58,703. The structural gene is flanked on both sides by palindromic structures followed by a stretch of T residues, suggesting transcriptional termination signals. Thus, it appears that protein A is translated from a monocistronic mRNA. The sequence reveals extensive internal homologies involving a 58-amino acid unit, responsible for IgG binding, repeated 5 times and an 8-amino acid unit, possibly responsible for binding to the cell wall of S. aureus, repeated 12 times. Comparisons between the repeated regions show a marked preference for silent mutations, indicating an evolutionary pressure to keep the amino acid sequence preserved. The structure of the gene also suggests how the gene has evolved.
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Metadata
- Authors
- Uhlén, M., Guss, B., Nilsson, B., Gatenbeck, S., Philipson, L., & Lindberg, M.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1984-02
- Volume
- 259
- Issue
- 3
- Pages
- 1695-1702
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W1601256941
- Cited by
- 566
- References
- 27
- FWCI
- 24.72
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Uhlén, M., Guss, B., Nilsson, B., Gatenbeck, S., Philipson, L., & Lindberg, M. (1984). Complete sequence of the staphylococcal gene encoding protein A. A gene evolved through multiple duplications. Journal of Biological Chemistry. https://doi.org/10.1016/s0021-9258(17)43463-6
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
