CoChat
Sign Up
Cited by 35Cell Adhesion Molecules ResearchSlime Mold and Myxomycetes ResearchGlycosylation and Glycoproteins Research

The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.

Adhesion of Dictyostelium discoideum cells to carbohydrates immobilized in polyacrylamide gels. I. Evidence for three sugar-specific cell surface receptors.

Open access
Bozzaro, S, Roseman, S·1983·Journal of Biological Chemistry
DOI 10.1016/s0021-9258(17)44000-2
Open this paper in CoChat — free account

Abstract

Dictyostelium discoideum cells appear to be able to recognize particular carbohydrate prosthetic groups at different stages in their life cycle. We therefore used our previously developed model system (consisting of polyacrylamide gels containing putative ligands covalently linked to the polymer) to determine the receptors on these cells capable of recognizing carbohydrates. D. discoideum cells, at different developmental stages from growth phase to late aggregation, were incubated with the derivatized gels, and the number of adherent cells was determined by measuring alanine transaminase after cell lysis. From 70 to 100% of the cells firmly adhered to gels derivatized with glucose, maltose, or cellobiose. The cells were also capable of binding to N-acetylglucosamine and mannose, but both the rate and the extent of binding to these sugars were less than those observed with the glucose derivatives. Furthermore, binding to N-acetylglucosamine decreased to negligible levels during the aggregation stage of development. The cells did not bind to the glucose-derivatized gels in the presence of glucose and a variety of carbohydrates containing glucose at the nonreducing termini, whereas binding was not inhibited by N-acetylglucosamine, mannose, and derivatives of these sugars. Adhesion to all sugars was blocked by 2,4-dinitrophenol. This inhibitor also reversed the binding to gels containing N-acetylglucosamine and mannose, but not to glucose. Differential binding to the three monosaccharides was also observed under conditions affecting the normal amoeboid shape of the cells. In addition, adhesion to N-acetylglucosamine and mannose was trypsin-sensitive, whereas adhesion to glucose was only slightly affected by treating the cells with trypsin (and cycloheximide). These and other results suggest that D. discoideum cell adhesion to derivatized gels is mediated by three different receptors, one highly specific for glucose and two (probably less specific) for N-acetylglucosamine and mannose.

Overview

Integrity signals

Registry

Crossref · 10.1016/s0021-9258(17)44000-2

Cites

1 of 1 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Verification

Claims the paper makes

We couldn’t retrieve this paper’s full text, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

  • Carbohydrate-binding receptors have been long suggested to be involved in Dictyostelium phagocytosis, though their identification has been elusive.

    “Carbohydrate-binding receptors have been long suggested to be involved in Dictyostelium phagocytosis, though their identification has been elusive so far [22,62,63].”

    From Genome-wide transcriptional changes induced by phagocytosis or growth on bacteria in Dictyostelium (2008) · cites this paper as [62]

Citations

Cites

Flagged first, then by citations

Research works printed but not linked: 41

  1. Roseman, 1970, Chem. Phys. Lipids, 5, 270, 10.1016/0009-3084(70)90024-1 · DOI
  2. Crandall, 1977, Vol. 3
  3. Lis, 1973, Annu. Rev. Biochem., 42, 541, 10.1146/annurev.bi.42.070173.002545 · DOI
  4. Olden, 1982, J. Cell. Biochem., 18, 313, 10.1002/jcb.1982.240180306 · DOI
  5. Turner, 1973, Nature (Lond.), 244, 509, 10.1038/244509a0 · DOI · Paper page
  6. Müller, 1978, Differentiation, 10, 55, 10.1111/j.1432-0436.1978.tb00945.x · DOI
  7. Oppenheimer, 1969, Proc. Natl. Acad. Sci. U. S. A., 63, 1395, 10.1073/pnas.63.4.1395 · DOI
  8. Balsamo, 1975, Biochemistry, 14, 167, 10.1021/bi00672a028 · DOI
  9. Barondes, 1981, Annu. Rev. Biochem., 50, 7, 10.1146/annurev.bi.50.070181.001231 · DOI
  10. Beug, 1973, J. Cell Biol., 56, 647, 10.1083/jcb.56.3.647 · DOI
  11. Bozzaro, 1978, J. Mol. Biol., 120, 265, 10.1016/0022-2836(78)90067-0 · DOI
  12. Müller, 1979, Eur. J. Biochem., 99, 419, 10.1111/j.1432-1033.1979.tb13271.x · DOI
  13. Bozzaro, 1981, Exp. Cell Res., 134, 181, 10.1016/0014-4827(81)90475-4 · DOI
  14. Steinemann, 1980, Nature (Lond.), 286, 621, 10.1038/286621a0 · DOI
  15. Wilcox, 1981, Proc. Natl. Acad. Sci. U. S. A., 78, 358, 10.1073/pnas.78.1.358 · DOI
  16. Saxe, 1982, J. Biol. Chem., 29, 755
  17. Lam, 1981, Differentiation, 20, 22, 10.1111/j.1432-0436.1981.tb01151.x · DOI
  18. Rosen, 1973, Proc. Natl. Acad. Sci. U. S. A., 70, 2554, 10.1073/pnas.70.9.2554 · DOI
  19. Rosen, 1975, Exp. Cell Res., 95, 159, 10.1016/0014-4827(75)90621-7 · DOI
  20. Cooper, 1981, J. Biol. Chem., 256, 5046, 10.1016/S0021-9258(19)69364-6 · DOI
  21. Rosen, 1979, Am. Zool., 19, 809, 10.1093/icb/19.3.809 · DOI
  22. Gerisch, 1980, Curr. Top. Dev. Biol., 14, 243, 10.1016/S0070-2153(08)60197-0 · DOI
  23. Shinnick, 1980, Proc. Natl. Acad. Sci. U. S. A., 77, 4788, 10.1073/pnas.77.8.4788 · DOI
  24. Town, 1970, Proc. Natl. Acad. Sci. U. S. A., 76, 308, 10.1073/pnas.76.1.308 · DOI
  25. Brookman, 1982, Dev. Biol., 91, 191, 10.1016/0012-1606(82)90022-7 · DOI
  26. Schnaar, 1978, J. Biol. Chem., 253, 7940, 10.1016/S0021-9258(17)34462-9 · DOI
  27. Weigel, 1979, J. Biol. Chem., 254, 10830, 10.1016/S0021-9258(19)86596-1 · DOI
  28. Weigel, 1979, Carbohydr. Res., 70, 83, 10.1016/S0008-6215(00)83272-3 · DOI
  29. Lee, 1979, Anal. Biochem., 95, 260, 10.1016/0003-2697(79)90215-X · DOI
  30. McElvy, 1969, Arch. Biophys. Biochem., 132, 99, 10.1016/0003-9861(69)90341-5 · DOI
  31. Rondle, 1955, Biochem. J., 61, 586, 10.1042/bj0610586 · DOI
  32. Gerisch, 1960, Roux’ Arch. Entwicklungsmech., 152, 632, 10.1007/BF00582043 · DOI
  33. Sussman, 1965, Biochem. Biophys. Res. Commun., 18, 763, 10.1016/0006-291X(65)90851-X · DOI
  34. Bozzaro, 1979, Cell Differ., 8, 117, 10.1016/0045-6039(79)90031-9 · DOI
  35. Marin, 1980, Dev. Biol., 80, 301, 10.1016/0012-1606(80)90406-6 · DOI
  36. Weeks, 1973, Exp. Cell Res., 76, 467, 10.1016/0014-4827(73)90406-0 · DOI
  37. Weeks, 1975, J. Biol. Chem., 250, 6706, 10.1016/S0021-9258(19)40990-3 · DOI
  38. Geltosky, 1976, Cell, 8, 391, 10.1016/0092-8674(76)90151-3 · DOI
  39. West, 1977, J. Cell Biol., 74, 264, 10.1083/jcb.74.1.264 · DOI
  40. Reitherman, 1975, Proc. Natl. Acad. Sci. U. S. A., 72, 3541, 10.1073/pnas.72.9.3541 · DOI
  41. Umbreit, 1975, J. Biol. Chem., 250, 9360, 10.1016/S0021-9258(19)40652-2 · DOI

Cited by

Sorted by citations

Metadata

Authors
Bozzaro, S., & Roseman, S.
Affiliations
Johns Hopkins University (US)
ORCID
Bozzaro, S
Venue
Journal of Biological Chemistry
Publisher
Elsevier BV
Published
1983-11
Volume
258
Issue
22
Pages
13882-13889
ISSN
0021-9258 (ISSN-L)
DOI
10.1016/s0021-9258(17)44000-2
OpenAlex
W1550374019
Cited by
35
References
1
Citations by year
2020: 1 · 2019: 2 · 2018: 2 · 2017: 1 · 2015: 1 · 2013: 1
FWCI
1.64
Type
journal article
Language
en
Open access
Licence
Locations
  • Journal of Biological Chemistry (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • Institutional Research Information System University of Turin (University of Turin) (submittedVersion)
Topics
  • Health Sciences › Medicine › Immunology and Allergy › Cell Adhesion Molecules Research
  • Life Sciences › Agricultural and Biological Sciences › Ecology, Evolution, Behavior and Systematics › Slime Mold and Myxomycetes Research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Glycosylation and Glycoproteins Research
Keywords
Dictyostelium discoideum, cell adhesion, carbohydrate receptors, cell surface receptors, glucose, N-acetylglucosamine, mannose, cellobiose, maltose, 2,4-dinitrophenol, trypsin sensitivity, cell aggregation, developmental stages
MeSH terms
Acetylglucosamine, Acrylic Resins, Carbohydrates, Carrier Proteins, Cell Adhesion, Dictyostelium, Dinitrophenols, Glucose, Kinetics, Maltose, Mannose, Monosaccharide Transport Proteins, Sodium Chloride, 2,4-Dinitrophenol, Mannose-Binding Lectins, Maltose-Binding Proteins

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review