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Binding of synthetic oligosaccharides to the hepatic Gal/GalNAc lectin. Dependence on fine structural features.
Open accessAbstract
A series of synthetic oligosaccharides, resembling natural N-acetyllactosamine type glycans, were tested for their ability to inhibit the binding of labeled ligand to the mammalian hepatic lectin on rabbit hepatocytes at 2 degrees C. A dramatic hierarchy of inhibitory potency (tetraantennary greater than triantennary much greater than biantennary much greater than monoantennary) could be demonstrated. The range of concentration required for 50% inhibition of labeled ligand binding extended from approximately 1 mM, for the monoantennary oligosaccharides, to approximately 1 nM for triantennary oligosaccharides, even though the absolute Gal concentration increased only 3-fold. It was found that the number of Gal residues/cluster and their branching mode are major determinants of binding affinity of ligands to the hepatic lectin on the surface of hepatocytes.
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“A single subcutaneous injection of trivalent GalNAc-conjugated siRNA can achieve efficacy for >12 months22, 2324.”
From A divalent siRNA chemical scaffold for potent and sustained modulation of gene expression throughout the central nervous system (2019) · cites this paper as [24]
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Metadata
- Authors
- Lee, Y. C., Townsend, R. R., Hardy, M. R., Lönngren, J., Arnarp, J., Haraldsson, M., & Lönn, H.
- Affiliations
- Johns Hopkins University (US)
- ORCID
- Haraldsson, M
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1983-01
- Volume
- 258
- Issue
- 1
- Pages
- 199-202
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W1595570412
- Cited by
- 503
- References
- 24
- Citations by year
- 2026: 7 · 2025: 6 · 2024: 10 · 2023: 18 · 2022: 11 · 2021: 15 · 2020: 17 · 2019: 9 · 2018: 10 · 2017: 4 · 2016: 7 · 2015: 13 · 2014: 14 · 2013: 13 · 2012: 19
- FWCI
- 5.24
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Journal of Biological Chemistry (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Glycosylation and Glycoproteins Research
- Physical Sciences › Chemistry › Organic Chemistry › Carbohydrate Chemistry and Synthesis
- Life Sciences › Immunology and Microbiology › Immunology › Galectins and Cancer Biology
- Keywords
- Gal/GalNAc lectin, synthetic oligosaccharides, N-acetyllactosamine, oligosaccharide branching, hepatocytes
- MeSH terms
- Animals, Binding, Competitive, Carbohydrate Sequence, Cell Membrane, Glycopeptides, Kinetics, Liver, Oligosaccharides, Rabbits, Structure-Activity Relationship, Lectins
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
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