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Import of proteins into mitochondria. Cytochrome b2 and cytochrome c peroxidase are located in the intermembrane space of yeast mitochondria.

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Daum, G, Böhni, P C, Schatz, G·1982·Journal of Biological Chemistry

Abstract

Yeast mitochondria were fractionated into inner membrane, outer membrane, matrix, and intermembrane space. Identity and purity of each fraction were monitored by enzyme assays, dodecyl sulfate-polyacrylamide gel electrophoresis, and immunological detection of characteristic mitochondrial polypeptides. Cytochrome b2 and cytochrome c peroxidase were found to be components of the intermembrane space. The most reliable marker of the outer membrane was a major 29,000-dalton polypeptide component. The availability of submitochondrial fractions provides a basis for studying import of precursor polypeptides into isolated yeast mitochondria.

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Metadata

Authors
Daum, G., Böhni, P. C., & Schatz, G.
Venue
Journal of Biological Chemistry
Publisher
Elsevier BV
Published
1982-11
Volume
257
Issue
21
Pages
13028-13033
ISSN
0021-9258 (ISSN-L)
OpenAlex
W1507851645
Cited by
1,132
References
0
FWCI
9.66
Type
journal article
Language
en
Open access
hybrid

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CoChat. (2026). Citation check: Import of proteins into mitochondria. Cytochrome b2 and cytochrome c peroxidase are located in the intermembrane space of yeast mitochondria. https://app.cochat.ai/papers/10.1016/s0021-9258(18)33617-2
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Daum, G., Böhni, P. C., & Schatz, G. (1982). Import of proteins into mitochondria. Cytochrome b2 and cytochrome c peroxidase are located in the intermembrane space of yeast mitochondria. Journal of Biological Chemistry. https://doi.org/10.1016/s0021-9258(18)33617-2

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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.