The DOI matches its Crossref record; 15 of the 15 references it cites were found in a registry or an index.
Botulinum neurotoxins are zinc proteins.
Open accessAbstract
The available amino acid sequences of 150-kDa botulinum and tetanus neurotoxins show the presence of a closely homologous segment in the middle of the light chain (NH2-terminal 50 kDa), which is the intracellularly active portion of the toxin. This segment contains the zinc binding motif of metalloendopeptidases, HEXXH. Atomic adsorption analysis of botulinum neurotoxins (serotypes A, B, and E) made on the basis of this observation demonstrated the presence of one zinc atom/molecule of 150-kDa neurotoxin. Conditions were found for the removal of the zinc ion with chelating agents and for the restoration of the normal metal content. The conserved segment, which includes the zinc binding motif, was synthesized and shown to bind [65Zn]2+. Chemical modification experiments indicated that two histidines and no cysteines are involved in Zn2+ coordination in agreement with a probable catalytic role for the zinc ion. The present findings suggest the possibility that botulinum neurotoxins are zinc proteases.
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Cites
Ellman, G. L. (1959) · Archives of Biochemistry and Biophysics · doi:10.1016/0003-9861(59)90090-6 · Cited by 26,311
Zinc coordination, function, and structure of zinc enzymes and other proteins
Vallee, B. L., & Auld, D. S. (1990) · Biochemistry · doi:10.1021/bi00476a001 · Cited by 1,777
[41] Modification of histidyl residues in proteins by diethylpyrocarbonate
Miles, E. W. (1977) · Methods in Enzymology · doi:10.1016/0076-6879(77)47043-5 · Cited by 801
Structural basis of the action of thermolysin and related zinc peptidases
Matthews, B. W. (1988) · Accounts of Chemical Research · doi:10.1021/ar00153a003 · Cited by 687
Hatheway, C. L. (1990) · Clinical Microbiology Reviews · doi:10.1128/cmr.3.1.66 · Cited by 650
Binding of Diffusible Molecules by Macromolecules: Rapid Measurement by Rate of Dialysis
Colowick, S. P., & Womack, F. C. (1969) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)94419-4 · Cited by 515
Arslan, P., Di Virgilio, F., Beltrame, M., Tsien, R. Y., & Pozzan, T. (1985) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)89421-2 · Cited by 485
Active-site zinc ligands and activated H2O of zinc enzymes.
Vallee, B. L., & Auld, D. S. (1990) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.87.1.220 · Cited by 437
How do tetanus and botulinum toxins bind to neuronal membranes?
Montecucco, C. (1986) · Trends in Biochemical Sciences · doi:10.1016/0968-0004(86)90282-3 · Cited by 400
Self-citationThree-dimensional Structure of Thermolysin
MATTHEWS, B. W., JANSONIUS, J. N., COLMAN, P. M., SCHOENBORN, B. P., & DUPOURQUE, D. (1972) · Nature New Biology · doi:10.1038/newbio238037a0 · Cited by 299
Cited by
Botulinum Neurotoxins: Biology, Pharmacology, and Toxicology
Pirazzini, M., Rossetto, O., Eleopra, R., & Montecucco, C. (2017) · Pharmacological Reviews · doi:10.1124/pr.116.012658 · Cited by 768
Self-citationVitale, G., Pellizzari, R., Recchi, C., Napolitani, G., Mock, M., & Montecucco, C. (1998) · Biochemical and Biophysical Research Communications · doi:10.1006/bbrc.1998.9040 · Cited by 403
Self-citationClostridial neurotoxins: new tools for dissecting exocytosis
Niemann, H., Blasi, J., & Jahn, R. (1994) · Trends in Cell Biology · doi:10.1016/0962-8924(94)90203-8 · Cited by 326
Klimpel, K. R., Arora, N., & Leppla, S. H. (1994) · Molecular Microbiology · doi:10.1111/j.1365-2958.1994.tb00500.x · Cited by 309
Nishiki, T., Kamata, Y., Nemoto, Y., Omori, A., Ito, T., Takahashi, M., & Kozaki, S. (1994) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(17)34087-5 · Cited by 305
Metadata
- Authors
- Schiavo, G., Rossetto, O., Santucci, A., DasGupta, B. R., & Montecucco, C.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1992-11
- Volume
- 267
- Issue
- 33
- Pages
- 23479-23483
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W4320301114
- Cited by
- 291
- References
- 15
- FWCI
- 14.22
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Schiavo, G., Rossetto, O., Santucci, A., DasGupta, B. R., & Montecucco, C. (1992). Botulinum neurotoxins are zinc proteins. Journal of Biological Chemistry. https://doi.org/10.1016/s0021-9258(18)35863-0
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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