Induction of four proteins in chick embryo cells by sodium arsenite.

Abstract

Four proteins of Mr = 89,000, 73,000, 35,000, and 27,000 are strongly induced in chick fibroblasts by sodium arsenite. Induction of these proteins is discoordinate as a function of arsenite concentration. Kinetically, all species appear 1 h after exposure to 50 microM arsenite, after 24 and 48 h of exposure, the 27,000 protein is still synthesized extensively, whereas normal cell proteins and the three other induced proteins are greatly reduced. The four proteins are unrelated by tryptic peptide-mapping procedures. Multiple subspecies of p89, p73, and p27 were observed in two-dimensional gels. The subspecies of p73 appear to be related as determined by partial proteolytic maps as are those of p27. Two-dimensional gel analysis of in vitro translation products from rabbit reticulocyte lysates primed with mRNA from uninduced and induced cells reveals that the amount of translatable mRNA specific for these proteins is increase by induction. This increase is attributable to new mRNA synthesis since actinomycin D prevent induction and new bands of RNA (Mr = 0.9 X 10(6) and 1.3 X 10(6)) appear in methyl mercury gels of oligo(dT) selected RNA from induced cells. These bands are assigned to p73 and p89 based on translation of electroeluted RNA from a similar preparative gel. A comparison is made between induction of these proteins and the heat shock response in Drosophilla melanogaster.