The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Nucleotide insertion kinetics opposite abasic lesions in DNA.
Open accessAbstract
A gel assay is introduced to measure DNA polymerase insertion kinetics at single sites along a DNA template strand. The assay is used to analyze the kinetics of inserting deoxynucleotides opposite a synthetic abasic (apurinic/apyrimidinic) lesions using Drosophila DNA polymerase alpha. The location of the abasic lesion next to different nearest-neighbor bases allows the effects of base stacking on the specificity of insertion to be evaluated. The specificity of nucleotide insertion, Vmax/Km, is 6-11 times greater for A over G and about 20-50 times greater for A over C and T. The insertion specificity at the abasic lesion appears to depend more on differences in Vmax than Km. Apparent Michaelis constants for inserting A and G deoxynucleotides are similar to within about a factor of 2. The insertion of A or G occurs most efficiently at the abasic lesion when T is the 5'-nearest neighbor on the primer strand and least efficiently when G is the 5'-nearest neighbor. The presence of different base stacking partners adjacent to the site of insertion has up to a 4-fold effect on specificity.
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Citations
Cites
Mutagenic deamination of cytosine residues in DNA
Duncan, B. K., & Miller, J. H. (1980) · Nature · doi:10.1038/287560a0 · Cited by 792
Cited by
Abasic sites in DNA: repair and biological consequences in Saccharomyces cerevisiae
Boiteux, S., & Guillet, M. (2004) · DNA Repair · doi:10.1016/j.dnarep.2003.10.002 · Cited by 502
Biochemical Basis of DNA Replication Fidelity
Goodman, M. F., Creighton, S., Bloom, L. B., Petruska, J., & Kunkel, T. A. (1993) · Critical Reviews in Biochemistry and Molecular Biology · doi:10.3109/10409239309086792 · Cited by 446
Self-citationThe major human abasic endonuclease: formation, consequences and repair of abasic lesions in DNA
Wilson, D. M., & Barsky, D. (2001) · Mutation Research/DNA Repair · doi:10.1016/s0921-8777(01)00063-5 · Cited by 408
DNA polymerase insertion fidelity. Gel assay for site-specific kinetics.
Boosalis, M. S., Petruska, J., & Goodman, M. F. (1987) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)47850-7 · Cited by 332
Self-citationMoran, S., Ren, R. X.-F., Rumney, & Kool, E. T. (1997) · Journal of the American Chemical Society · doi:10.1021/ja963718g · Cited by 317
Metadata
- Authors
- Randall, S. K., Eritja, R., Kaplan, B. E., Petruska, J., & Goodman, M. F.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1987-05
- Volume
- 262
- Issue
- 14
- Pages
- 6864-6870
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W1497215682
- Cited by
- 211
- References
- 1
- FWCI
- 4.49
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Randall, S. K., Eritja, R., Kaplan, B. E., Petruska, J., & Goodman, M. F. (1987). Nucleotide insertion kinetics opposite abasic lesions in DNA. Journal of Biological Chemistry. https://doi.org/10.1016/s0021-9258(18)48324-x
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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