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Regulation of the transferrin-independent iron transport system in cultured cells.
Open accessAbstract
Mammalian cells accumulate iron via the binding of transferrin to high affinity surface receptors, or through a transferrin-independent pathway which involves the uptake of iron-organic anion chelates by a membrane-based transport system. Previously we determined that the transferrin-independent transport system was present on a wide variety of cultured cells (Sturrock, A., Alexander, J., Lamb, J., Craven, C. M., and Kaplan, J. (1990) J. Biol. Chem. 265, 3139-3145). In this communication we demonstrate that the transferrin-independent iron uptake system is regulated differently than the transferrin-mediated pathway. The activity of the transferrin-independent system was unaffected by changes in cellular growth rate, induction of DNA synthesis and cell division, or depletion of cellular iron. Exposure of cells to ferric or ferrous iron, however, resulted in a time-dependent increase in transport activity, due to a change in Vmax with no change in Km. Increased transport activity was seen in a variety of cultured cell types, occurred in the presence of cycloheximide, and persisted for hours after removal of iron. The ability of other transition metals to induce changes in transport, or to compete with iron for accumulation by the transferrin-independent uptake system, was critically dependent on the composition of the media in which the cells were incubated. Metals such as Cu2+ or Zn2+, but not Cd2+ or Mn2+, when dissolved in a balanced salt solution buffered with 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid, induced changes in the transferrin-independent iron transport system. The same metals which induced changes in transport were ineffective in media containing amino acids, ascorbate, or N-[2-hydroxy-1,1-bis(hydroxymethyl)ethyl]glycine. The Vmax of the transferrin-independent iron transport system was also elevated by increases in intracellular Ca2+. The effect of iron on transport activity, however, did not result from an iron-induced release of intracellular Ca2+. These results suggest a novel form of regulation in which the presence of extracellular iron induces the appearance of previously cryptic transporters and thus accelerates the clearance of potentially toxic molecules.
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“Studies of non-transferrin bound iron uptake by cultured cells support this conclusion.”
From Forging a field: the golden age of iron biology. (2008) · cites this paper as [69]
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Cited by
Sorted by citations
Fleming, M. D., Romano, M. A., Su, M. A., Garrick, L. M., Garrick, M. D., & Andrews, N. C. (1998) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.95.3.1148 · Cited by 936
The Evolution of Iron Chelators for the Treatment of Iron Overload Disease and Cancer
Kalinowski, D. S., & Richardson, D. R. (2005) · Pharmacological Reviews · doi:10.1124/pr.57.4.2 · Cited by 721
“…endocytosis remains the major pathway of iron uptake, cells can also internalize iron from small molecular weight complexes such as ferric citrate through a membrane-based iron transport system (Sturrock et al., 1990; Kaplan et al., 1991; Richardson and Baker, 1991; Richardson and Ponka, 1995).”
BackgroundNon-transferrin bound iron: a key role in iron overload and iron toxicity
Brissot, P., Ropert, M., Le Lan, C., & Loréal, O. (2012) · Biochimica et Biophysica Acta (BBA) - General Subjects · doi:10.1016/j.bbagen.2011.07.014 · Cited by 654
“Using transformed cell lines (fibroblasts [23], HepG2 cells [24]) intracellular iron loading led to an increased NTBI uptake.”
Forging a field: the golden age of iron biology.
Andrews, N. C. (2008) · Blood · doi:10.1182/blood-2007-12-077388 · Cited by 623
Gunshin, H., Fujiwara, Y., Custodio, A. O., DiRenzo, C., Robine, S., & Andrews, N. C. (2005) · Journal of Clinical Investigation · doi:10.1172/jci24356 · Cited by 419
“The non–transferrin-bound iron transport system appears to require reduction of Fe3+ to Fe2+ by an extracellular ferrireductase (10, 11, 30).”
Background
Metadata
- Authors
- Kaplan, J., Jordan, I., & Sturrock, A.
- Affiliations
- University of Utah (US)
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1991-02
- Volume
- 266
- Issue
- 5
- Pages
- 2997-3004
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W1587837383
- Cited by
- 198
- References
- 26
- Citations by year
- 2026: 2 · 2024: 1 · 2023: 1 · 2021: 1 · 2020: 2 · 2019: 2 · 2018: 8 · 2016: 3 · 2015: 3 · 2014: 4 · 2013: 8 · 2012: 5
- FWCI
- 6.12
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Journal of Biological Chemistry (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health (5r01dk020630-22)
- National Institute of Diabetes and Digestive and Kidney Diseases (DK20630, DK30534)
- Topics
- Health Sciences › Medicine › Hematology › Iron Metabolism and Disorders
- Health Sciences › Nursing › Nutrition and Dietetics › Trace Elements in Health
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Micronutrient Interactions and Effects
- Keywords
- iron uptake regulation, transferrin receptor, extracellular iron, copper, zinc, intracellular calcium, iron accumulation, cultured cells
- MeSH terms
- Biological Transport, Cadmium, Calcium, Copper, Cycloheximide, Ferritins, HeLa Cells, Humans, Iron, Manganese, Transferrin, Zinc
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