The DOI matches its Crossref record; 37 of the 37 references it cites were found in a registry or an index.
Contrasting mechanisms for suppression of macrophage cytokine release by transforming growth factor-beta and interleukin-10
Open accessAbstract
Transforming growth factor (TGF)-beta and interleukin (IL)-10 inhibited lipopolysaccharide (LPS)-induced macrophage production of the inflammatory cytokines tumor necrosis factor-alpha (TNF), IL-1 alpha, and IL-1 beta by contrasting post-transcriptional mechanisms. TGF-beta acted slowly and late, as it required 12-16 h to exert a suppressive effect, and inhibited TNF production even when added 6 h after LPS. TGF-beta affected neither the level of TNF mRNA, the release of preformed TNF nor the degradation of TNF. Thus, TGF-beta appeared to inhibit translation of TNF mRNA. IL-10 not only suppressed TNF release to a 25-fold greater extent than TGF-beta, but also inhibited release of IL-1. In contrast to TGF-beta, IL-10 acted on an early step in cytokine production, its effect being maximal 3 h after addition of LPS. Unlike TGF-beta, IL-10 markedly suppressed TNF, IL-1 alpha, and IL-1 beta mRNA levels. However, this was accomplished without suppressing transcription of the corresponding genes. Moreover, cycloheximide antagonized the IL-10-dependent reduction in cytokine mRNA levels. Thus, IL-10 may induce a ribonuclease active on cytokine transcripts or may induce a protein that enhances the susceptibility of TNF, IL-1 alpha, and IL-1 beta mRNAs to ribonucleolytic action. We conclude that IL-10 and TGF-beta induce different phenotypes of macrophage deactivation, and deactivate macrophages by different mechanisms: IL-10 promotes degradation of cytokine mRNA, while TGF-beta primarily suppresses translation.
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Macrophage activation and polarization
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The anti-inflammatory effect of exercise
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Muscle as an Endocrine Organ: Focus on Muscle-Derived Interleukin-6
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Metadata
- Authors
- Bogdan, C., Paik, J., Vodovotz, Y., & Nathan, C.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1992-11
- Volume
- 267
- Issue
- 32
- Pages
- 23301-23308
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W1606157168
- Cited by
- 512
- References
- 37
- FWCI
- 9.84
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Bogdan, C., Paik, J., Vodovotz, Y., & Nathan, C. (1992). Contrasting mechanisms for suppression of macrophage cytokine release by transforming growth factor-beta and interleukin-10. Journal of Biological Chemistry. https://doi.org/10.1016/s0021-9258(18)50091-0
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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