The DOI matches its Crossref record; 48 of the 48 references it cites were found in a registry or an index.
Induction of Sp1-p53 DNA-binding heterocomplexes during granulocyte/macrophage colony-stimulating factor-dependent proliferation in human erythroleukemia cell line TF-1.
Open accessAbstract
The involvement of Sp1 in regulating cell proliferation in myeloid leukemia cells was determined by measuring the levels and DNA binding activity of Sp1 in TF-1 cells, a human erythroleukemia cell line dependent on granulocyte/macrophage colony-stimulating factor (GM-CSF) for viability and cell growth. DNA binding of Sp1 to a specific double-stranded oligodeoxynucleotide was increased markedly in a dose-dependent manner in proliferating cells in response to GM-CSF compared with growth-arrested or apoptotic cells. Competition experiments and mobility shift interference assays with antibodies against Sp1 as well as wild-type or mutant p53 indicated that GM-CSF-inducible DNA-binding complexes contained both Sp1 and p53 and that these heterocomplexes bound to both p53- and Sp1-binding sequences with high affinity. Immunoprecipitation of nuclear extracts with a p53 antibody indicated that Sp1 was associated as a heterocomplex with p53. Formation of this complex was dependent on the level of p53 since p53 was more abundant in proliferating cells and decreased upon induction of growth arrest and apoptosis by withdrawal of GM-CSF while Sp1 levels remained unchanged. These results suggest that the association of Sp1 with p53 may represent a novel mechanism of growth regulation in cytokine-dependent leukemia cells.
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“In 1993, p53 was first reported to bind to coactivators, including the TATA-box binding protein,73, 74 the CCAAT-box binding factor (NF-Y)75 and specificity protein 1 (Sp1) that binds to GC-boxes,76 and to interfere with their transactivator function.”
From Census and evaluation of p53 target genes (2017) · cites this paper as [76]
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Metadata
- Authors
- Borellini, F., & Glazer, R. I.
- Affiliations
- Georgetown University Medical Center (US)
- ORCID
- Glazer, R.I.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1993-04
- Volume
- 268
- Issue
- 11
- Pages
- 7923-7928
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W1547769073
- Cited by
- 147
- References
- 48
- Citations by year
- 2025: 1 · 2022: 1 · 2018: 1 · 2017: 2 · 2016: 3 · 2014: 4 · 2013: 1 · 2012: 2
- FWCI
- 4.98
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Journal of Biological Chemistry (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health (5r01ca054231-03)
- National Cancer Institute (CA54231)
- Topics
- Health Sciences › Medicine › Oncology › Cancer-related Molecular Pathways
- Health Sciences › Medicine › Hematology › Acute Myeloid Leukemia Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Keywords
- Sp1, p53, heterocomplexes, DNA binding, GM-CSF, erythroleukemia, TF-1 cell line, cell proliferation, growth regulation, apoptosis, growth arrest, transcription factor binding sites
- MeSH terms
- Base Sequence, Cell Division, Cell Nucleus, Chromatin, DNA-Binding Proteins, DNA, Neoplasm, Leukemia, Erythroblastic, Acute, Humans, Microscopy, Electron, Molecular Sequence Data, Oligodeoxyribonucleotides, Tumor Cells, Cultured, Tumor Suppressor Protein p53, Granulocyte-Macrophage Colony-Stimulating Factor, Sp1 Transcription Factor, Apoptosis
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