The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Cloning, sequencing, purification, and Gq-dependent activation of phospholipase C-beta 3.
Open accessAbstract
Six mammalian phospholipase C isozymes (PLC-beta 1, PLC-beta 2, PLC-gamma 1, PLC-gamma 2, PLC-delta 1, and PLC-delta 2) have been identified at both protein and DNA levels. Here, cDNAs corresponding to a previously unidentified PLC isozyme were isolated from a rat thyroid cell FRTL cDNA library. Comparison of the predicted amino acid sequence of this new PLC with other known PLC isozymes revealed a high degree of overall similarity with PLC-beta 1 and PLC-beta 2. Thus, the new PLC was named PLC-beta 3. Comparison with PLC-beta 1 and PLC-beta 2 also revealed that the deduced amino-terminal sequence of PLC-beta 3 was incomplete by 10-20 amino acids. With the use of antibodies raised against synthetic peptides corresponding to PLC-beta 3-specific amino acid sequences, we purified PLC-beta 3 from a rat brain particulate fraction. The purified enzyme exhibited an apparent molecular mass of 152 kDa on SDS-polyacrylamide gels, as compared with 150 and 140 kDa for PLC-beta 1 and PLC-beta 2, respectively. Studies of the activation of PLC-beta isozymes by three alpha subunits of Gq class G proteins, alpha q, alpha 11, and alpha 16 in the presence of guanosine 5-O-(3-thiotriphosphate) (GTP gamma S) revealed that the extent of activation decreased in the order of PLC-beta 1 > or = PLC-beta 3 >> PLC-beta 2 for all three alpha subunits, suggesting a certain degree of specificity in the interaction of Gq alpha subunits with different PLC-beta isozymes.
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Cites
Activation of the β1 isozyme of phospholipase C by α subunits of the Gq class of G proteins
Taylor, S. J., Chae, H. Z., Rhee, S. G., & Exton, J. H. (1991) · Nature · doi:10.1038/350516a0 · Cited by 780
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Cited by
Regulation of Phosphoinositide-Specific Phospholipase C
Rhee, S. G. (2001) · Annual Review of Biochemistry · doi:10.1146/annurev.biochem.70.1.281 · Cited by 1,628
Self-citationMultiple roles of phosphoinositide-specific phospholipase C isozymes
Suh, P.-G., Park, J.-I., Manzoli, L., Cocco, L., Peak, J. C., Katan, M., Fukami, K., Kataoka, T., Yun, S.-U., & Ryu, S.-H. (2008) · BMB Reports · doi:10.5483/bmbrep.2008.41.6.415 · Cited by 501
Offermanns, S., Laugwitz, K. L., Spicher, K., & Schultz, G. (1994) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.91.2.504 · Cited by 465
The Concise Guide to PHARMACOLOGY 2013/14: Enzymes
Alexander, S. P. H., Benson, H. E., Faccenda, E., Pawson, A. J., Sharman, J. L., Spedding, M., Peters, J. A., Harmar, A. J., & Collaborators, C. (2013) · British Journal of Pharmacology · doi:10.1111/bph.12451 · Cited by 425
REGULATION OF EUKARYOTIC PHOSPHATIDYLINOSITOL-SPECIFIC PHOSPHOLIPASE C AND PHOSPHOLIPASE D
Singer, W. D., Brown, H. A., & Sternweis, P. C. (1997) · Annual Review of Biochemistry · doi:10.1146/annurev.biochem.66.1.475 · Cited by 401
Metadata
- Authors
- Jhon, D. Y., Lee, H. H., Park, D., Lee, C. W., Lee, K. H., Yoo, O. J., & Rhee, S. G.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1993-03
- Volume
- 268
- Issue
- 9
- Pages
- 6654-6661
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W1522961358
- Cited by
- 212
- References
- 1
- FWCI
- 8.74
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Jhon, D. Y., Lee, H. H., Park, D., Lee, C. W., Lee, K. H., Yoo, O. J., & Rhee, S. G. (1993). Cloning, sequencing, purification, and Gq-dependent activation of phospholipase C-beta 3. Journal of Biological Chemistry. https://doi.org/10.1016/s0021-9258(18)53300-7
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