The DOI matches its Crossref record; 50 of the 50 references it cites were found in a registry or an index.
Pteridine biosynthesis in human endothelial cells. Impact on nitric oxide-mediated formation of cyclic GMP.
Open accessAbstract
Stimulation of nitric oxide (NO) synthase in endothelial cells by Ca2+ influx leads to increased intracellular levels of cGMP. NO synthase from various sources is known to use tetrahydrobiopterin, flavins, and NADPH as cofactors. We studied the effect of interferon-gamma, tumor necrosis factor-alpha, and lipopolysaccharide on tetrahydrobiopterin biosynthetic activities in human umbilical vein endothelial cells (HUVEC). These stimuli led to an up to 40-fold increase of GTP cyclohydrolase I (EC 3.5.4.16) activity and to increased accumulation of neopterin and tetrahydrobiopterin in HUVEC. Further enzyme activities of tetrahydrobiopterin biosynthesis, i.e. 6-pyruvoyl tetrahydropterin synthase and sepiapterin reductase (EC 1.1.1.153), remained unchanged. NO synthase activity in protein fractions from homogenates of cells treated with interferon-gamma plus tumor necrosis factor-alpha was not influenced as compared with untreated controls. However, interferon-gamma alone or in combination with tumor necrosis factor-alpha significantly increased intracellular cGMP formation in intact HUVEC by 50 and 80%, respectively. These stimuli increased intracellular tetrahydrobiopterin concentrations up to 14-fold. NO-triggered cGMP formation was similarly increased by incubation of otherwise untreated cells with sepiapterin, leading to elevated intracellular tetrahydrobiopterin levels. Thus, cytokines indirectly stimulate the activity of constitutive NO synthase in HUVEC by upregulating production of the cofactor tetrahydrobiopterin.
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“Increased NOX1 activity is associated with vascular disease, and studies have shown links between high NOX1 levels and hypertension (1015), diabetes (163, 380, 1003), ischemia/reperfusion injury (479), and coronary artery disease (393).”
From Sources of Vascular Nitric Oxide and Reactive Oxygen Species and Their Regulation (2019) · cites this paper as [1003]
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Cites
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Research works printed but not linked: 2
- Werner-Felmayer, 1990, Cancer Res., 50, 2863
- Brown, 1989, Cancer Res., 49, 4941
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Nitric oxide synthases: regulation and function
Forstermann, U., & Sessa, W. C. (2012) · European Heart Journal · doi:10.1093/eurheartj/ehr304 · Cited by 4,409
Endothelial Nitric Oxide Synthase in Vascular Disease: From Marvel to Menace
Förstermann, U., & Münzel, T. (2006) · Circulation · doi:10.1161/circulationaha.105.602532 · Cited by 1,957
Regulation of biosynthesis of nitric oxide.
Nathan, C., & Xie, Q. W. (1994) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(17)36703-0 · Cited by 1,669
Nitric oxide and oxidative stress in vascular disease
Förstermann, U. (2010) · Pflügers Archiv - European Journal of Physiology · doi:10.1007/s00424-010-0808-2 · Cited by 796
Cytokine regulation of endothelial cell function
Mantovani, A., Bussolino, F., & Dejana, E. (1992) · The FASEB Journal · doi:10.1096/fasebj.6.8.1592209 · Cited by 677
Metadata
- Authors
- Werner-Felmayer, G., Werner, E. R., Fuchs, D., Hausen, A., Reibnegger, G., Schmidt, K., Weiss, G., & Wachter, H.
- Affiliations
- Universität Innsbruck (AT)
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1993-01
- Volume
- 268
- Issue
- 3
- Pages
- 1842-1846
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W1496789829
- Cited by
- 263
- References
- 50
- Citations by year
- 2023: 1 · 2022: 1 · 2021: 1 · 2020: 1 · 2019: 1 · 2018: 3 · 2017: 3 · 2016: 4 · 2015: 2 · 2014: 4 · 2013: 3 · 2012: 6
- FWCI
- 13.33
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Journal of Biological Chemistry (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Physiology (medical) › Nitric Oxide and Endothelin Effects
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Biochemical and Molecular Research
- Health Sciences › Nursing › Nutrition and Dietetics › Folate and B Vitamins Research
- Keywords
- pteridine synthesis, tetrahydrobiopterin, nitric oxide synthase, cyclic GMP, endothelial cells, GTP cyclohydrolase I, interferon-gamma, TNF-α, lipopolysaccharide, neopterin, 6-pyruvoyl-tetrahydropterin synthase, sepiapterin reductase, sepiapterin
- MeSH terms
- Amino Acid Oxidoreductases, Biopterins, Cells, Cultured, Endothelium, Vascular, GTP Cyclohydrolase, Cyclic GMP, Humans, Hypoxanthines, Interferon-gamma, Lipopolysaccharides, Nitric Oxide, Pteridines, Pterins, Tumor Necrosis Factor-alpha, Umbilical Veins, Nitric Oxide Synthase, Neopterin
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