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In vivo regulation of monomer-tetramer conversion of pyruvate kinase subtype M2 by glucose is mediated via fructose 1,6-bisphosphate

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Ashizawa, K., Willingham, M.C., Liang, C.M., Cheng, S.Y.·1991·Journal of Biological Chemistry

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Abstract

The activity of pyruvate kinase, subtype M2 (PKM2), is known to be increased by fructose 1,6-bisphosphate (Fru-1,6-P2), one of the metabolites in the glycolytic pathway. Recently, we have shown that in vitro, Fru-1,6-P2 activated the association of monomer to form the tetrameric PKM2. To ascertain whether this mode of regulation also occurs in vivo, we prepared monomer-specific monoclonal antibody and quantified the monomer formation in situ in cultured cells by immunocytochemistry. The intracellular Fru-1,6-P2 was manipulated by the glucose concentration in the media. At the physiological concentration of glucose (4-6 mM), 30-35% of PK existed as a monomer. However, PKM2 was dissociated into monomer within minutes after cells were deprived of glucose. The maximal level of monomer was detected after 1 h at 37 degrees C. Monomer was rapidly (within minutes) converted to tetramer after addition of glucose. Furthermore, when cells cultured in 10 mM of glucose were treated with cytochalasin B, an inhibitor of the glucose transporter, a maximal level of monomer was detected within 20-30 min. Determination of Fru-1,6-P2 indicated that its intracellular concentration decreased concomitantly with the reduction in glucose concentration in the medium. These results indicate that monomer-tetramer inter-conversion is a major in vivo cellular regulatory mechanism in response to changes in the extracellular glucose concentration via Fru-1,6-P2.

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Authors: Ashizawa, K., Willingham, M. C., Liang, C. M., & Cheng, S. Y.
Affiliations: National Institutes of Health (US)
ORCID: Willingham, M.C.
Liang, C.M.
Cheng, S.Y.
Venue: Journal of Biological Chemistry
Publisher: Elsevier BV
Published: 1991-09
Volume: 266
Issue: 25
Pages: 16842-16846
ISSN: 0021-9258 (ISSN-L)
DOI: 10.1016/s0021-9258(18)55378-3
OpenAlex: W1484073306
Cited by: 172
References: 12
Citations by year: 2026: 8 · 2025: 3 · 2024: 4 · 2023: 6 · 2022: 6 · 2021: 16 · 2020: 5 · 2019: 13 · 2018: 9 · 2017: 11 · 2016: 8 · 2015: 6 · 2014: 8 · 2013: 14 · 2012: 16
FWCI: 1.06
Type: journal article
Language: en
Open access: hybrid
doi.org
Licence: cc-by
www.elsevier.com/tdm/userlicense/1.0/ (tdm)
www.elsevier.com/legal/tdmrep-license (tdm)
creativecommons.org/licenses/by/4.0/ (vor, 10636 d)
Locations: Journal of Biological Chemistry (publishedVersion, cc-by)
PubMed (publishedVersion)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Metabolism, Diabetes, and Cancer
Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Cancer, Hypoxia, and Metabolism
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Biochemical and Molecular Research
Keywords: PKM2, fructose-1,6-bisphosphate, glucose regulation, glucose deprivation, cytochalasin B, glucose transporter inhibition, enzyme oligomerization, glycolysis, monoclonal antibodies
MeSH terms: Thyroid Hormone-Binding Proteins, Animals, Antibodies, Monoclonal, Carrier Proteins, Cell Line, Cytochalasin B, Fluorescent Antibody Technique, Fructosediphosphates, Glucose, Humans, Kinetics, Membrane Proteins, Precipitin Tests, Pyruvate Kinase, Thyroid Hormones, Triiodothyronine, Macromolecular Substances

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In vivo regulation of monomer-tetramer conversion of pyruvate kinase subtype M2 by glucose is mediated via fructose 1,6-bisphosphate

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