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Cited by 27

The DOI matches its Crossref record; 45 of the 45 references it cites were found in a registry or an index.

Cell-specific Expression of Mouse Albumin Promoter

Izban, M G, Papaconstantinou, J·1989·Journal of Biological Chemistry
DOI 10.1016/s0021-9258(18)60510-1
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Abstract

Regulation of albumin gene expression is believed to be mediated by multiple nuclear factors that interact with cis-acting DNA sequences within the first 160 base pairs (bp) of the promoter. The minimal promoter sequence required to generate tissue-specific expression has not been clearly defined. We have constructed a series of transient expression vectors containing progressive deletions of the mouse albumin gene 5'-flanking sequence fused to the bacterial chloramphenicol acetyltransferase (CAT) gene and include the Moloney murine leukemia viral (Mo-MuLV) enhancer. Promoter activity was determined in mouse hepatoma and fibroblast cell lines by chloramphenicol acetyltransferase and S1 nuclease analyses. All constructions were compared with -623 Albcat-Mo-MuLV which contains all the sequence homology between the rat and mouse promoters. Low levels of expression were observed with -60 Albcat-Mo-MuLV (10%) in hepatoma but not fibroblast cells. Addition of promoter sequence to -208 bp progressively increased activity to 190% in the hepatoma cells, while -308 and -1612 Albcat-Mo-MuLV had activity similar to the -623 Albcat-Mo-MuLV level, and -3000 Albcat-Mo-MuLV showed a 2-fold reduction in transcriptional activity. The inclusion of promoter sequences upstream of -60 generated low levels of expression in the fibroblasts. We also show that factors from mouse liver nuclear extracts protect at least five regions of the albumin promoter upstream of -160. Our results indicate that tis…

Overview

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Crossref · 10.1016/s0021-9258(18)60510-1

Cites

45 of 45 references verified0 citation errors · 0 not checkable

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Citations

Cites

Research works printed but not linked: 8

  1. Grayson, 1988, Mol. Cell. Biol., 8, 1055
  2. Godbout, 1986, Mol. Cell. Biol., 6, 477
  3. Costa, 1986, Mol. Cell. Biol., 6, 4697
  4. Heard, 1987, Mol. Cell. Biol., 7, 2425
  5. Babiss, 1986, Biologist, 6, 3798
  6. Gorman, 1982, Mol. Cell. Biol., 2, 1044
  7. Hall, 1983, J. Mol. Biol., 2, 101
  8. Godbout, 1988, Mol. Cell. Biol., 8, 1169

Cited by

See all 27

Metadata

Authors
Izban, M. G., & Papaconstantinou, J.
Venue
Journal of Biological Chemistry
Publisher
Elsevier BV
Published
1989-06
Volume
264
Issue
16
Pages
9171-9179
ISSN
0021-9258
DOI
10.1016/s0021-9258(18)60510-1
Cited by
27
References
45
Language
en
Licence

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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.