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Molecular Weight Determination of Protein-Dodecyl Sulfate Complexes by Gel Electrophoresis in a Discontinuous Buffer System
Open accessAbstract
This report describes methods and results obtained by combining the techniques of sodium dodecyl sulfate (SDS) gel electrophoresis and electrophoresis in discontinuous buffer systems. The SDS gel system utilizes a sulfate-borate discontinuity which stacks and unstacks protein-SDS complexes over a range of 2,300 to 320,000 daltons, providing high resolution fractionation. The properties of protein-SDS complexes are investigated by calculating retardation coefficients and apparent free mobilities from Ferguson plots. Apparent free mobilities are approximately constant, establishing a linear relationship between the logarithm of the relative mobility and the retardation coefficient. The retardation coefficient is shown both empirically and theoretically to be a uniform function of molecular weight of protein-SDS complexes over specified ranges, providing a rationale for determining molecular weight from plots of the negative logarithm of relative mobility against molecular weight.
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Cites
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
LAEMMLI, U. K. (1970) · Nature · doi:10.1038/227680a0 · Cited by 249,961
Cited by
Lehrach, H., Diamond, D., Wozney, J. M., & Boedtker, H. (1977) · Biochemistry · doi:10.1021/bi00640a033 · Cited by 3,415
Characterization of membrane proteins in detergent solutions
Tanford, C., & Reynolds, J. A. (1976) · Biochimica et Biophysica Acta (BBA) - Reviews on Biomembranes · doi:10.1016/0304-4157(76)90009-5 · Cited by 854
Protein blotting: Principles and applications
Gershoni, J. M., & Palade, G. E. (1983) · Analytical Biochemistry · doi:10.1016/0003-2697(83)90128-8 · Cited by 853
Weiss, J., Elsbach, P., Olsson, I., & Odeberg, H. (1978) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(17)40872-6 · Cited by 451
Utermann, G., Jaeschke, M., & Menzel, J. (1975) · FEBS Letters · doi:10.1016/0014-5793(75)81125-2 · Cited by 337
Metadata
- Authors
- Neville, D. M.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1971-10
- Volume
- 246
- Issue
- 20
- Pages
- 6328-6334
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W1566240917
- Cited by
- 1,989
- References
- 1
- FWCI
- 47.85
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Neville, D. M. (1971). Molecular Weight Determination of Protein-Dodecyl Sulfate Complexes by Gel Electrophoresis in a Discontinuous Buffer System. Journal of Biological Chemistry. https://doi.org/10.1016/s0021-9258(18)61792-2
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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