The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Hydrolysis of Proteins with p-Toluenesulfonic Acid
Open accessAbstract
Abstract A new procedure for analyses for tryptophan in proteins and glycoproteins is described. The method utilizes p-toluenesulfonic acid as the catalyst for hydrolysis and the amino acid analyzer for the quantitative estimation of tryptophan. The hydrolysis of proteins is carried out in 3 n p-toluenesulfonic acid containing 0.2% 3-(2-aminoethyl)-indole in evacuated sealed tubes at 110° for 22, 48, and 72 hours. The values of tryptophan obtained were close to the expected integral values and the recoveries of all other amino acids were comparable to those observed after hydrolysis with 6 n HCl. Since the hydrolysate can be placed on the ion exchange column, without the prior removal of the solvent which is required when 6 n HCl is used for the hydrolysis, the present procedure is more convenient than the use of 6 n HCl. This procedure, however, is not designed for the analyses of proteins that are grossly contaminated with carbohydrates such as feeds. The limit of carbohydrate content that the present procedure can tolerate is 2.0 mg when 2 to 4 mg of protein are being hydrolyzed with 1.0 ml of the acid.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Sanger, F., & Thompson, E. O. P. (1953) · Biochemical Journal · doi:10.1042/bj0530353 · Cited by 567
Cited by
Complete amino acid analysis of proteins from a single hydrolysate.
Simpson, R. J., Neuberger, M. R., & Liu, T. Y. (1976) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(17)33637-2 · Cited by 990
Self-citationThe purification, composition, and specificity of the anti-T lectin from peanut (Arachis hypogaea).
Lotan, R., Skutelsky, E., Danon, D., & Sharon, N. (1975) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)40790-4 · Cited by 939
A new acid hydrolysis method for determining tryptophan in peptides and proteins
Penke, B., Ferenczi, R., & Kovács, K. (1974) · Analytical Biochemistry · doi:10.1016/0003-2697(74)90129-8 · Cited by 608
Hydrolysis and amino acid composition analysis of proteins
Fountoulakis, M., & Lahm, H.-W. (1998) · Journal of Chromatography A · doi:10.1016/s0021-9673(98)00721-3 · Cited by 606
Entactin, a novel basal lamina-associated sulfated glycoprotein.
Carlin, B., Jaffe, R., Bender, B., & Chung, A. E. (1981) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)69388-9 · Cited by 444
Metadata
- Authors
- Liu, T.-Y., & Chang, Y. H.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1971-05
- Volume
- 246
- Issue
- 9
- Pages
- 2842-2848
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W1597014450
- Cited by
- 1,043
- References
- 1
- FWCI
- 253.94
- Type
- journal article
- Language
- en
- Open access
- hybrid
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Hydrolysis of Proteins with p-Toluenesulfonic Acid. https://app.cochat.ai/papers/10.1016/s0021-9258(18)62259-8
- The paper
- Liu, T.-Y., & Chang, Y. H. (1971). Hydrolysis of Proteins with p-Toluenesulfonic Acid. Journal of Biological Chemistry. https://doi.org/10.1016/s0021-9258(18)62259-8
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
