The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Site-directed cross-linking. Establishing the dimeric structure of the aspartate receptor of bacterial chemotaxis.
Open accessAbstract
Cysteine residues introduced at specific locations in the aspartate receptor of Salmonella typhimurium provide anchor points for cross-linking and serve as chemical markers for structural studies of this oligomeric receptor. These markers have been used to measure the rate of subunit exchange between oligomeric receptors and to show that ligand binding inhibits this exchange. The cysteine-containing receptors can be oxidatively cross-linked to completion within the oligomeric receptor, indicating that the receptor has an even number of subunits. Based on this observation, a technique has been developed that can be used to determine the oligomeric structure of proteins under a variety of experimental conditions. The technique involves the measurement of the effect of dilution by "cysteineless" receptor subunits on cross-linking and reveals that the aspartate receptor is dimeric in detergent solution, in a mixed-micelle system, and in reconstituted membrane vesicles. Binding of aspartate does not change the oligomeric structure of the receptor, indicating that transmembrane signaling occurs within an oligomeric receptor of constant size.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Yarden, Y., & Schlessinger, J. (1987) · Biochemistry · doi:10.1021/bi00379a035 · Cited by 627
Cited by
Signal transduction schemes of bacteria
Parkinson, J. S. (1993) · Cell · doi:10.1016/0092-8674(93)90267-t · Cited by 772
Bacterial chemoreceptors: high-performance signaling in networked arrays
Hazelbauer, G. L., Falke, J. J., & Parkinson, J. S. (2008) · Trends in Biochemical Sciences · doi:10.1016/j.tibs.2007.09.014 · Cited by 632
Milburn, M. V., Privé, G. G., Milligan, D. L., Scott, W. G., Yeh, J., Jancarik, J., Koshland, D. E., & Kim, S.-H. (1991) · Science · doi:10.1126/science.1660187 · Cited by 411
Self-citationCloning of a putative glutamate receptor: A low affinity kainate-binding subunit
Bettler, B., Egebjerg, J., Sharma, G., Pecht, G., Hermans-Borgmeyer, I., Moll, C., Stevens, C. F., & Heinemann, S. (1992) · Neuron · doi:10.1016/0896-6273(92)90292-l · Cited by 377
Gegner, J. A., Graham, D. R., Roth, A. F., & Dahlquist, F. W. (1992) · Cell · doi:10.1016/0092-8674(92)90247-a · Cited by 338
Metadata
- Authors
- Milligan, D. L., & Koshland, D. E.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1988-05
- Volume
- 263
- Issue
- 13
- Pages
- 6268-6275
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W2101755633
- Cited by
- 232
- References
- 1
- FWCI
- 1.91
- Type
- journal article
- Language
- en
- Open access
- hybrid
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Site-directed cross-linking. Establishing the dimeric structure of the aspartate receptor of bacterial chemotaxis. https://app.cochat.ai/papers/10.1016/s0021-9258(18)68781-2
- The paper
- Milligan, D. L., & Koshland, D. E. (1988). Site-directed cross-linking. Establishing the dimeric structure of the aspartate receptor of bacterial chemotaxis. Journal of Biological Chemistry. https://doi.org/10.1016/s0021-9258(18)68781-2
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
