The DOI matches its Crossref record; 31 of the 31 references it cites were found in a registry or an index.
Three-Dimensional Structures of the Ligand-Binding Domain of the Bacterial Aspartate Receptor with and Without a Ligand
Open accessAbstract
The three-dimensional structure of an active, disulfide cross-linked dimer of the ligand-binding domain of the " Salmonella typhimurium aspartate receptor and that of an aspartate complex have been determined by x-ray crystallographic methods at 2.4 and 2.0 angstrom (Å) resolution, respectively. A single subunit is a four-α-helix bundle with two long amino-terminal and carboxyl-terminal helices and two shorter helices that form a cylinder 20 Å in diameter and more than 70 Å long. The two subunits in the disulfide-bonded dimer are related by a crystallographic twofold axis in the apo structure, but by a noncrystallographic twofold axis in the aspartate complex structure. The latter structure reveals that the ligand binding site is located more than 60 Å from the presumed membrane surface and is at the interface of the two subunits. Aspartate binds between two α helices from one subunit and one α helix from the other in a highly charged pocket formed by three arginines. The comparison of the apo and aspartate complex structures shows only small structural changes in the individual subunits, except for one loop region that is disordered, but the subunits appear to change orientation relative to each other. The structures of the two forms of this protein provide a step toward understanding the mechanisms of transmembrane signaling.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Kabsch, W., & Sander, C. (1983) · Biopolymers · doi:10.1002/bip.360221211 · Cited by 15,320
Ullrich, A., Coussens, L., Hayflick, J. S., Dull, T. J., Gray, A., Tam, A. W., Lee, J., Yarden, Y., Libermann, T. A., Schlessinger, J., et al. (1984) · Nature · doi:10.1038/309418a0 · Cited by 2,752
Structure of the haemagglutinin membrane glycoprotein of influenza virus at 3 Å resolution
Wilson, I. A., Skehel, J. J., & Wiley, D. C. (1981) · Nature · doi:10.1038/289366a0 · Cited by 2,525
X-ray structure analysis of a membrane protein complex
Deisenhofer, J., Epp, O., Miki, K., Huber, R., & Michel, H. (1984) · Journal of Molecular Biology · doi:10.1016/s0022-2836(84)80011-x · Cited by 1,926
Wiley, D. C., Wilson, I. A., & Skehel, J. J. (1981) · Nature · doi:10.1038/289373a0 · Cited by 1,615
The human insulin receptor cDNA: The structural basis for hormone-activated transmembrane signalling
Ebina, Y., Ellis, L., Jarnagin, K., Edery, M., Graf, L., Clauser, E., Ou, J.-H., Masiarz, F., Kan, Y. W., Goldfine, I. D., et al. (1985) · Cell · doi:10.1016/0092-8674(85)90334-4 · Cited by 1,477
Resolution of phase ambiguity in macromolecular crystallography
Wang, B.-C. (1985) · Methods in Enzymology · doi:10.1016/0076-6879(85)15009-3 · Cited by 1,028
Structure of the reaction center from Rhodobacter sphaeroides R-26: the cofactors.
Allen, J. P., Feher, G., Yeates, T. O., Komiya, H., & Rees, D. C. (1987) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.84.16.5730 · Cited by 915
An efficient general-purpose least-squares refinement program for macromolecular structures
Tronrud, D. E., Ten Eyck, L. F., & Matthews, B. W. (1987) · Acta Crystallographica Section A Foundations of Crystallography · doi:10.1107/s0108767387099124 · Cited by 847
Three-Dimensional Structure of the LDL Receptor-Binding Domain of Human Apolipoprotein E
Wilson, C., Wardell, M. R., Weisgraber, K. H., Mahley, R. W., & Agard, D. A. (1991) · Science · doi:10.1126/science.2063194 · Cited by 678
Cited by
Signal transduction schemes of bacteria
Parkinson, J. S. (1993) · Cell · doi:10.1016/0092-8674(93)90267-t · Cited by 772
Stimulus Perception in Bacterial Signal-Transducing Histidine Kinases
Mascher, T., Helmann, J. D., & Unden, G. (2006) · Microbiology and Molecular Biology Reviews · doi:10.1128/mmbr.00020-06 · Cited by 721
Polar Location of the Chemoreceptor Complex in the Escherichia coli Cell
Maddock, J. R., & Shapiro, L. (1993) · Science · doi:10.1126/science.8456299 · Cited by 708
Spivak-Kroizman, T., Lemmon, M. A., Dikic, I., Ladbury, J. E., Pinchasi, D., Huang, J., Jaye, M., Crumley, G., Schlessinger, J., & Lax, I. (1994) · Cell · doi:10.1016/0092-8674(94)90032-9 · Cited by 658
Bacterial chemoreceptors: high-performance signaling in networked arrays
Hazelbauer, G. L., Falke, J. J., & Parkinson, J. S. (2008) · Trends in Biochemical Sciences · doi:10.1016/j.tibs.2007.09.014 · Cited by 632
Metadata
- Authors
- Milburn, M. V., Privé, G. G., Milligan, D. L., Scott, W. G., Yeh, J., Jancarik, J., Koshland, D. E., & Kim, S.-H.
- Venue
- Science
- Published
- 1991-11-29
- Volume
- 254
- Issue
- 5036
- Pages
- 1342-1347
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2082928276
- Cited by
- 411
- References
- 31
- FWCI
- 9.51
- Type
- journal article
- Language
- en
- Open access
- green
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Three-Dimensional Structures of the Ligand-Binding Domain of the Bacterial Aspartate Receptor with and Without a Ligand. https://app.cochat.ai/papers/10.1126/science.1660187
- The paper
- Milburn, M. V., Privé, G. G., Milligan, D. L., Scott, W. G., Yeh, J., Jancarik, J., Koshland, D. E., & Kim, S.-H. (1991). Three-Dimensional Structures of the Ligand-Binding Domain of the Bacterial Aspartate Receptor with and Without a Ligand. Science. https://doi.org/10.1126/science.1660187
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
