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Photochemically generated cytosolic calcium pulses and their detection by fluo-3
Open accessAbstract
Fluo-3, one member of a family of new fluorescent Ca2+ indicators excitable at wavelengths in the visible (Minta, A., Kao, J. P. Y., and Tsien, R. Y. (1989) J. Biol. Chem. 264, 8171-8178), has been tested in living cells. We demonstrate that fluo-3 can be loaded into fibroblasts and lymphocytes by incubation with the pentaacetoxymethyl ester of the dye and that the ester is hydrolyzed intracellularly to yield genuine fluo-3 capable of indicating changes in [Ca2+]i induced by agonist stimulation. Fluo-3 can also be microinjected into fibroblasts along with photolabile compounds such as nitr-5 and caged inositol trisphosphate for photorelease experiments. Fluo-3 permits continuous monitoring of [Ca2+]i without interference with use of UV-sensitive caged compounds. A procedure for combined use of ionophore and heavy metal ions in end-of-experiment calibration of fluo-3 intensities to give [Ca2+]i is also described.
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Cited by
Glutamate Induces Calcium Waves in Cultured Astrocytes: Long-Range Glial Signaling
Cornell-Bell, A. H., Finkbeiner, S. M., Cooper, M. S., & Smith, S. J. (1990) · Science · doi:10.1126/science.1967852 · Cited by 1,846
Cyclic ADP-Ribose in Insulin Secretion from Pancreatic βCells
Takasawa, S., Nata, K., Yonekura, H., & Okamoto, H. (1993) · Science · doi:10.1126/science.8420005 · Cited by 441
Estimating Intracellular Calcium Concentrations and Buffering without Wavelength Ratioing
Maravall, M., Mainen, Z. F., Sabatini, B. L., & Svoboda, K. (2000) · Biophysical Journal · doi:10.1016/s0006-3495(00)76809-3 · Cited by 416
Leinekugel, X., Medina, I., Khalilov, I., Ben-Ari, Y., & Khazipov, R. (1997) · Neuron · doi:10.1016/s0896-6273(00)80265-2 · Cited by 407
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Kawashima, K. (2000) · Pharmacology & Therapeutics · doi:10.1016/s0163-7258(99)00071-6 · Cited by 384
Metadata
- Authors
- Kao, J. P. Y., Harootunian, A. T., & Tsien, R. Y.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1989-05
- Volume
- 264
- Issue
- 14
- Pages
- 8179-8184
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W1529590189
- Cited by
- 811
- References
- 0
- FWCI
- 44.34
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Kao, J. P. Y., Harootunian, A. T., & Tsien, R. Y. (1989). Photochemically generated cytosolic calcium pulses and their detection by fluo-3. Journal of Biological Chemistry. https://doi.org/10.1016/s0021-9258(18)83166-0
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