The DOI matches its Crossref record; 24 of the 24 references it cites were found in a registry or an index.
Internalization and recycling of activated thrombin receptors
Open accessAbstract
Shortly after activation by either thrombin or the tethered ligand domain peptide SFLLRN, thrombin receptors undergo homologous desensitization, temporarily losing their ability to respond to both agonists. We have examined the role of receptor internalization and recycling in this process using receptor-directed antibodies as probes. The results show within 1 min of activation > 85% of the approximately 200,000 thrombin receptors on megakaryoblastic human erythroleukemia (HEL) and CHRF-288 cells are sequestered into endosomes via coated pits, after which the majority are transferred to lysosomes. This process does not require proteolysis of the receptor and occurs with sufficient speed to play a major role in the regulation of thrombin receptor function. Although most of the internalized receptors are ultimately degraded, approximately 25% return to the cell surface. These recycled receptors are in a state in which they can respond to SFLLRN but not thrombin; nor do they self-activate despite the apparent continued presence of the tethered ligand. In contrast to other G protein-coupled receptors, which are internalized and then recycled in an activatable state, recovery of the thrombin response occurs only after the expression on the cell surface of adequate numbers of newly synthesized receptors.
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Cites
Continuous cultures of fused cells secreting antibody of predefined specificity
KÖHLER, G., & MILSTEIN, C. (1975) · Nature · doi:10.1038/256495a0 · Cited by 17,229
Vu, T.-K. H., Hung, D. T., Wheaton, V. I., & Coughlin, S. R. (1991) · Cell · doi:10.1016/0092-8674(91)90261-v · Cited by 2,970
von Zastrow, M., & Kobilka, B. K. (1992) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)50762-1 · Cited by 459
Beta-adrenergic receptor sequestration. A potential mechanism of receptor resensitization.
Yu, S. S., Lefkowitz, R. J., & Hausdorff, W. P. (1993) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)54155-7 · Cited by 390
Glickman, J. N., Conibear, E., & Pearse, B. M. (1989) · The EMBO Journal · doi:10.1002/j.1460-2075.1989.tb03471.x · Cited by 333
Vassallo, R. R., Kieber-Emmons, T., Cichowski, K., & Brass, L. F. (1992) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)42664-6 · Cited by 299
Self-citationInhibition of fibrinogen binding to stimulated human platelets by a monoclonal antibody.
Bennett, J. S., Hoxie, J. A., Leitman, S. F., Vilaire, G., & Cines, D. B. (1983) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.80.9.2417 · Cited by 271
Self-citationTabilio, A., Rosa, J. P., Testa, U., Kieffer, N., Nurden, A. T., Del Canizo, M. C., Breton-Gorius, J., & Vainchenker, W. (1984) · The EMBO Journal · doi:10.1002/j.1460-2075.1984.tb01827.x · Cited by 224
Parton, R. G., Prydz, K., Bomsel, M., Simons, K., & Griffiths, G. (1989) · The Journal of cell biology · doi:10.1083/jcb.109.6.3259 · Cited by 222
Self-citationWaldo, G. L., Northup, J. K., Perkins, J. P., & Harden, T. K. (1983) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(17)44003-8 · Cited by 204
Cited by
Characterization of Protease-activated Receptor-2 Immunoreactivity in Normal Human Tissues
R. D'Andrea, M., Derian, C. K., Leturcq, D., Baker, S. M., Brunmark, A., Ling, P., Darrow, A. L., Santulli, R. J., Brass, L. F., & Andrade–Gordon, P. (1998) · Journal of Histochemistry & Cytochemistry · doi:10.1177/002215549804600204 · Cited by 320
Self-citationFörster, R., Kremmer, E., Schubel, A., Breitfeld, D., Kleinschmidt, A., Nerl, C., Bernhardt, G., & Lipp, M. (1998) · The Journal of Immunology · doi:10.4049/jimmunol.160.3.1522 · Cited by 253
Protease-activated receptors in health and disease
Peach, C. J., Edgington-Mitchell, L. E., Bunnett, N. W., & Schmidt, B. L. (2023) · Physiological Reviews · doi:10.1152/physrev.00044.2021 · Cited by 116
YAZAWA, H., YU, Z.-X., TAKEDA, K., LE, Y., GONG, W., FERRANS, V. J., OPPENHEIM, J. J., LI, C. C. H., & WANG, J. M. (2001) · The FASEB Journal · doi:10.1096/fj.01-0251com · Cited by 105
Metadata
- Authors
- Hoxie, J. A., Ahuja, M., Belmonte, E., Pizarro, S., Parton, R., & Brass, L. F.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1993-06
- Volume
- 268
- Issue
- 18
- Pages
- 13756-13763
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W1545475398
- Cited by
- 272
- References
- 24
- FWCI
- 6.37
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Hoxie, J. A., Ahuja, M., Belmonte, E., Pizarro, S., Parton, R., & Brass, L. F. (1993). Internalization and recycling of activated thrombin receptors. Journal of Biological Chemistry. https://doi.org/10.1016/s0021-9258(18)86921-6
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
