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Carboxyl-terminal isoprenylation of ras-related GTP-binding proteins encoded by rac1, rac2, and ralA

Kinsella, B.T., Erdman, R.A., Maltese, W.A.·1991·Journal of Biological Chemistry
DOI 10.1016/s0021-9258(18)92889-9
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Abstract

Membrane localization of p21ras is dependent upon its posttranslational modification by a 15-carbon farnesyl group. The isoprenoid is linked to a cysteine located within a conserved carboxyl-terminal sequence termed the "CAAX" box (where C is cysteine, A is an aliphatic amino acid, and X is any amino acid). We now show that three GTP-binding proteins encoded by the recently identified rac1, rac2, and ralA genes also undergo isoprenoid modification. cDNAs coding for each protein were transcribed in vitro, and the RNAs were translated in reticulocyte lysates. Incorporation of isoprenoid precursors, [3H]mevalonate or [3H]farnesyl pyrophosphate, indicated that the translation products were modified by isoprenyl groups. A protein recognized by an antibody to rac1 also comigrated with a protein metabolically labeled by a product of [3H] mevalonate in cultured cells. Gel permeation chromatography of radiolabeled hydrocarbons released from the rac1, rac2, and ralA proteins by reaction with Raney nickel catalyst indicated that unlike p21Hras, which was modified by a 15-carbon moiety, the rac and ralA translation products were modified by 20-carbon isoprenyl groups. Site-directed mutagenesis established that the isoprenylated cysteines in the rac1, rac2, and ralA proteins were located in the fourth position from the carboxyl terminus. The three-amino acid extension distal to the cysteine was required for this modification. The isoprenylation of rac1 (CSLL), ralA (CCIL), and the site-di…

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Research works printed but not linked: 63

  1. Maltese, 1990, FASEB J., 4, 3319, 10.1096/fasebj.4.15.2123808 · DOI
  2. Glomset, 1990, Trends Biochem. Sei., 15, 139, 10.1016/0968-0004(90)90213-U · DOI
  3. Maltese, 1989, J. Biol. Chem., 264, 18168, 10.1016/S0021-9258(19)84692-6 · DOI
  4. Farnsworth, 1989, J. Biol. Chem., 264, 20422, 10.1016/S0021-9258(19)47079-8 · DOI
  5. Reiss, 1990, Cell, 62, 81, 10.1016/0092-8674(90)90242-7 · DOI
  6. Schaber, 1990, J. Biol. Chem., 265, 14701, 10.1016/S0021-9258(18)77164-0 · DOI
  7. Fukada, 1990, Nature, 346, 658, 10.1038/346658a0 · DOI
  8. Lai, 1990, Proc. Natl. Acad. Sei. U. S. A., 87, 7673, 10.1073/pnas.87.19.7673 · DOI
  9. Rilling, 1990, Science, 247, 318, 10.1126/science.2296720 · DOI
  10. Farnsworth, 1990, Science, 247, 320, 10.1126/science.2296721 · DOI
  11. Mumby, 1990, Proc. Natl. Acad. Sei. U. S. A., 87, 5873, 10.1073/pnas.87.15.5873 · DOI
  12. Yamane, 1990, Proc. Natl. Acad. Sei. U. S. A., 87, 5868, 10.1073/pnas.87.15.5868 · DOI
  13. Kawata, 1990, Proc. Natl. Acad. Sei. U. S. A., 87, 8960, 10.1073/pnas.87.22.8960 · DOI
  14. Hancock, 1989, Cell, 57, 1167, 10.1016/0092-8674(89)90054-8 · DOI
  15. Casey, 1989, Proc. Natl. Acad. Sei. U. S. A., 86, 8323, 10.1073/pnas.86.21.8323 · DOI
  16. Schafer, 1989, Science, 245, 379, 10.1126/science.2569235 · DOI
  17. Leonard, 1990, J. Biol. Chem., 265, 5157, 10.1016/S0021-9258(19)34099-2 · DOI
  18. Manne, 1990, Proc. Natl. Acad. Sei. U. S. A., 87, 7541, 10.1073/pnas.87.19.7541 · DOI
  19. Kim, 1990, Mol. Cell. Biol., 10, 5945
  20. Maltese, 1990, J. Biol. Chem., 265, 17883, 10.1016/S0021-9258(18)38246-2 · DOI
  21. Yamane, 1991, Proc. Natl. Acad. Sei. U. S. A., 88, 286, 10.1073/pnas.88.1.286 · DOI
  22. Wolda, 1988, J. Biol. Chem., 263, 5997, 10.1016/S0021-9258(18)68736-8 · DOI
  23. Beck, 1988, J. Cell Biol., 107, 1307, 10.1083/jcb.107.4.1307 · DOI
  24. Vorburger, 1989, EMBO J., 8, 4007, 10.1002/j.1460-2075.1989.tb08583.x · DOI
  25. Pollard, 1990, Mol. Cell. Biol., 10, 2164
  26. Beck, 1990, J. Cell Biol., 110, 1489, 10.1083/jcb.110.5.1489 · DOI
  27. Maltese, 1990, J. Biol. Chem., 265, 18071, 10.1016/S0021-9258(17)44715-6 · DOI
  28. Schmidt, 1984, J. Biol. Chem., 259, 10175, 10.1016/S0021-9258(18)90945-2 · DOI
  29. Sinensky, 1985, Proc. Natl. Acad. Sei. U. S. A., 82, 3257, 10.1073/pnas.82.10.3257 · DOI
  30. Maltese, 1987, J. Cell. Physiol., 133, 471, 10.1002/jcp.1041330307 · DOI
  31. Repko, 1989, J. Biol. Chem., 264, 9945, 10.1016/S0021-9258(18)81751-3 · DOI
  32. Sepp-Lorenzino, 1989, FEBS Lett., 245, 110, 10.1016/0014-5793(89)80202-9 · DOI
  33. Maltese, 1990, J. Biol. Chem., 265, 2148, 10.1016/S0021-9258(19)39953-3 · DOI
  34. Santos, 1989, FASEB J., 3, 2151, 10.1096/fasebj.3.10.2666231 · DOI
  35. Hall, 1990, Science, 249, 635, 10.1126/science.2116664 · DOI
  36. Bourne, 1990, Nature, 348, 125, 10.1038/348125a0 · DOI
  37. Keller, 1986, J. Biol. Chem., 261, 12053, 10.1016/S0021-9258(18)67201-1 · DOI
  38. Didsbury, 1989, J. Biol. Chem., 264, 16378, 10.1016/S0021-9258(19)84716-6 · DOI · Paper page
  39. Polakis, 1989, J. Biol. Chem., 264, 16383, 10.1016/S0021-9258(19)84717-8 · DOI
  40. Hemsley, 1989, Nucleic Acid Res., 17, 6545, 10.1093/nar/17.16.6545 · DOI
  41. Chen, 1985, DNA (New York), 4, 165
  42. Suzuki, 1988, Anal. Biochem., 72, 259, 10.1016/0003-2697(88)90440-X · DOI
  43. Garrels, 1979, J. Biol. Chem., 254, 7961, 10.1016/S0021-9258(18)36039-3 · DOI
  44. Chardin, 1986, EMBO J., 5, 2203, 10.1002/j.1460-2075.1986.tb04485.x · DOI · Paper page
  45. Chardin, 1989, Nucleic Acid Res., 17, 4380, 10.1093/nar/17.11.4380 · DOI
  46. Anderegg, 1988, J. Biol. Chem., 263, 18236, 10.1016/S0021-9258(19)81351-0 · DOI
  47. Jones, 1990, J. Biol. Chem., 265, 19389, 10.1016/S0021-9258(17)45381-6 · DOI
  48. Saito, 1979, J. Biol. Chem., 254, 8511, 10.1016/S0021-9258(19)86921-1 · DOI
  49. Sagami, 1981, Biochem. Int., 3, 669
  50. Didsbury, 1990, Biochem. Biophys. Res. Commun., 171, 804, 10.1016/0006-291X(90)91217-G · DOI
  51. Reese, 1991, Mol. Cell. Biochem.
  52. Gautam, 1989, Science, 224, 971, 10.1126/science.2499046 · DOI
  53. Robishaw, 1989, J. Biol. Chem., 264, 15758, 10.1016/S0021-9258(18)71540-8 · DOI
  54. Pizon, 1988, Nucleic Acids Res., 16, 7719, 10.1093/nar/16.15.7719 · DOI
  55. Shinjo, 1990, Proc. Natl. Acad. Sei. U. S. A., 87, 9853, 10.1073/pnas.87.24.9853 · DOI
  56. Clarke, 1988, Proc. Natl. Acad. Sei. U. S. A., 85, 4643, 10.1073/pnas.85.13.4643 · DOI
  57. Lowe, 1990, Oncogene, 5, 1045
  58. Schaefer, 1990, Science, 249, 1133, 10.1126/science.2204115 · DOI
  59. Stephenson, 1990, J. Biol. Chem., 265, 16248, 10.1016/S0021-9258(17)46215-6 · DOI
  60. Holtz, 1989, Cell, 59, 969, 10.1016/0092-8674(89)90753-8 · DOI
  61. Krohne, 1989, J. Cell Biol., 109, 2003, 10.1083/jcb.109.5.2003 · DOI
  62. Shirsat, 1990, Oncogene, 5, 769
  63. Bhullar, 1990, FEBS. Lett., 260, 48, 10.1016/0014-5793(90)80063-O · DOI

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Metadata

Authors
Kinsella, B. T., Erdman, R. A., & Maltese, W. A.
Venue
Journal of Biological Chemistry
Publisher
Elsevier BV
Published
1991-05
Volume
266
Issue
15
Pages
9786-9794
ISSN
0021-9258
DOI
10.1016/s0021-9258(18)92889-9
Cited by
131
References
0
Language
en
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CoChat. (2026). Citation check: Carboxyl-terminal isoprenylation of ras-related GTP-binding proteins encoded by rac1, rac2, and ralA. https://app.cochat.ai/papers/10.1016/s0021-9258(18)92889-9
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Kinsella, B. T., Erdman, R. A., & Maltese, W. A. (1991). Carboxyl-terminal isoprenylation of ras-related GTP-binding proteins encoded by rac1, rac2, and ralA. Journal of Biological Chemistry. https://doi.org/10.1016/s0021-9258(18)92889-9