Agonist-modulated Regulation of AMP-activated Protein Kinase (AMPK) in Endothelial Cells
Open accessAbstract
The endothelial isoform of nitric-oxide synthase (eNOS), a key determinant of vascular homeostasis, is a calcium/calmodulin-dependent phosphoprotein regulated by diverse cell surface receptors. Vascular endothelial growth factor (VEGF) and sphingosine 1-phosphate (S1P) stimulate eNOS activity through Akt/phosphoinositide 3-kinase and calcium-dependent pathways. AMP-activated protein kinase (AMPK) also activates eNOS in endothelial cells; however, the molecular mechanisms linking agonist-mediated AMPK regulation with eNOS activation remain incompletely understood. We studied the role of AMPK in VEGF- and S1P-mediated eNOS activation and found that both agonists led to a striking increase in AMPK phosphorylation in pathways involving the calcium/calmodulin-dependent protein kinase kinase beta. Treatment with tyrosine kinase inhibitors or the phosphoinositide 3-kinase inhibitor wortmannin demonstrated differential effects of VEGF versus S1P. Small interfering RNA (siRNA)-mediated knockdown of AMPKalpha1or Akt1 impaired the stimulatory effects of both VEGF and S1P on eNOS activation. AMPKalpha1 knockdown impaired agonist-mediated Akt phosphorylation, whereas Akt1 knockdown did not affect AMPK activation, thus suggesting that AMPK lies upstream of Akt in the pathway leading from receptor activation to eNOS stimulation. Importantly, we found that siRNA-mediated knockdown of AMPKalpha1 abrogates agonist-mediated activation of the small GTPase Rac1. Conversely, siRNA-mediated knockdown of Rac1 decreased the agonist-mediated phosphorylation of AMPK substrates without affecting that of AMPK, implicating Rac1 as a molecular link between AMPK and Akt in agonist-mediated eNOS activation. Finally, siRNA-mediated knockdown of caveolin-1 significantly enhanced AMPK phosphorylation, suggesting that AMPK is negatively regulated by caveolin-1. Taken together, these results suggest that VEGF and S1P differentially regulate AMPK and establish a central role for an agonist-modulated AMPK --> Rac1 --> Akt axis in the control of eNOS in endothelial cells.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
AMPK lies upstream of the phosphatidylinositol-3-kinase-protein kinase B pathway, which also leads to eNOS activation and NO production.
“Further evidence also shows that AMPK lies upstream of the phosphatidylinositol-3-kinase-protein kinase B pathway, which also leads to eNOS activation and NO production [162,164,165].”
From AMPK, Mitochondrial Function, and Cardiovascular Disease (2020) · cites this paper as [162]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Cites
2 retracted
Activation of the AMP-activated Protein Kinase by the Anti-diabetic Drug Metformin in Vivo
Zou, M., Kirkpatrick, S. S., Davis, B. J., Nelson, J. S., Wiles, W. G., Schlattner, U., Neumann, D., Brownlee, M., Freeman, M. B., & Goldman, M. H. (2004) · Journal of Biological Chemistry · doi:10.1074/jbc.m404421200 · Cited by 471 · see citing works
RetractedZou, M., Hou, X., Shi, C., Kirkpatick, S., Liu, F., Goldman, M. H., & Cohen, R. A. (2003) · Journal of Biological Chemistry · doi:10.1074/jbc.m300215200 · Cited by 167 · see citing works
RetractedDuplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells
Elbashir, S. M., Harborth, J., Lendeckel, W., Yalcin, A., Weber, K., & Tuschl, T. (2001) · Nature · doi:10.1038/35078107 · Cited by 9,456 · see citing works
Role of AMP-Activated Protein Kinase in Mechanism of Metformin Action
Zhou, G., Myers, R., Li, Y., Chen, Y., Shen, X., Fenyk-Melody, J., Wu, M., Ventre, J., Doebber, T., Fujii, N., et al. (2001) · Journal of Clinical Investigation · doi:10.1172/jci13505 · Cited by 5,652 · see citing works
Yamauchi, T., Kamon, J., Minokoshi, Y., Ito, Y., Waki, H., Uchida, S., Yamashita, S., Noda, M., Kita, S., Ueki, K., et al. (2002) · Nature Medicine · doi:10.1038/nm788 · Cited by 4,238 · see citing works
Regulation of endothelium-derived nitric oxide production by the protein kinase Akt
Fulton, D. J., Gratton, J., McCabe, T. J. D., Fontana, J. T., Fujio, Y., Walsh, K., Franke, T. F., Papapetropoulos, A., & Sessa, W. C. (1999) · Nature · doi:10.1038/21218 · Cited by 2,612 · see citing works
Caveolin, a protein component of caveolae membrane coats
Rothberg, K. G., Heuser, J. E., Donzell, W. C., Ying, Y., Glenney, J. R., & Anderson, R. G. W. (1992) · Cell · doi:10.1016/0092-8674(92)90143-z · Cited by 2,259 · see citing works
Shaw, R. J., Kosmatka, M., Bardeesy, N., Hurley, R. L., Witters, L. A., DePinho, R. A., & Cantley, L. C. (2004) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0308061100 · Cited by 1,797 · see citing works
Burridge, K., & Wennerberg, K. (2004) · Cell · doi:10.1016/s0092-8674(04)00003-0 · Cited by 1,790 · see citing works
Hawley, S. A., Pan, D. A., Mustard, K. J., Ross, L., Bain, J., Edelman, A. M., Frenguelli, B. G., & Hardie, D. G. (2005) · Cell Metabolism · doi:10.1016/j.cmet.2005.05.009 · Cited by 1,589 · see citing works
The AMP‐Activated Protein Kinase: Fuel Gauge of the Mammalian Cell?
Hardie, D. G., & Carling, D. (1997) · European Journal of Biochemistry · doi:10.1111/j.1432-1033.1997.00259.x · Cited by 1,317 · see citing works
Folkman, J., & Haudenschild, C. C. (1980) · Nature · doi:10.1038/288551a0 · Cited by 1,044 · see citing works
The Ca2+/Calmodulin-dependent Protein Kinase Kinases Are AMP-activated Protein Kinase Kinases
Hurley, R. L., Anderson, K. A., Franzone, J. M., Kemp, B. E., Means, A. R., & Witters, L. A. (2005) · Journal of Biological Chemistry · doi:10.1074/jbc.m503824200 · Cited by 1,021 · see citing works
Adiponectin Stimulates Production of Nitric Oxide in Vascular Endothelial Cells
Chen, H., Montagnani, M., Funahashi, T., Shimomura, I., & Quon, M. J. (2003) · Journal of Biological Chemistry · doi:10.1074/jbc.m307878200 · Cited by 993 · see citing works
AMP‐activated protein kinase phosphorylation of endothelial NO synthase
Chen, Z.-P., Mitchelhill, K. I., Michell, B. J., Stapleton, D., Rodríguez‐Crespo, I., Witters, L. A., Power, D. A., de Montellano, P. R. O., & Kemp, B. E. (1999) · FEBS Letters · doi:10.1016/s0014-5793(98)01705-0 · Cited by 835 · see citing works
THE FINE STRUCTURE OF THE GALL BLADDER EPITHELIUM OF THE MOUSE
Yamada, E. (1955) · The Journal of Cell Biology · doi:10.1083/jcb.1.5.445 · Cited by 816 · see citing works
Ouchi, N., Kobayashi, H., Kihara, S., Kumada, M., Sato, K., Inoue, T., Funahashi, T., & Walsh, K. (2004) · Journal of Biological Chemistry · doi:10.1074/jbc.m310389200 · Cited by 759 · see citing works
Russell, R. R., Li, J., Coven, D. L., Pypaert, M., Zechner, C., Palmeri, M., Giordano, F. J., Mu, J., Birnbaum, M. J., & Young, L. H. (2004) · Journal of Clinical Investigation · doi:10.1172/jci19297 · Cited by 738 · see citing works
Angiogenesis Assays: A Critical Overview
Auerbach, R., Lewis, R. L., Shinners, B., Kubai, L., & Akhtar, N. (2003) · Clinical Chemistry · doi:10.1373/49.1.32 · Cited by 705 · see citing works
Parton, R. G. (1996) · Current Opinion in Cell Biology · doi:10.1016/s0955-0674(96)80033-0 · Cited by 551 · see citing works
Rho Family GTPases Bind to Phosphoinositide Kinases
Tolias, K. F., Cantley, L. C., & Carpenter, C. L. (1995) · Journal of Biological Chemistry · doi:10.1074/jbc.270.30.17656 · Cited by 466 · see citing works
Akt1 regulates pathological angiogenesis, vascular maturation and permeability in vivo
Chen, J., Somanath, P. R., Razorenova, O. V., Chen, W. S., Hay, N., BORNSTEIN, P. K., & Byzova, T. V. (2005) · Nature Medicine · doi:10.1038/nm1307 · Cited by 450 · see citing works
Regulation of acetyl-CoA carboxylase
Brownsey, R. W., Boone, A. N., Elliott, J. E., Kulpa, J. E., & Lee, W. M. (2006) · Biochemical Society Transactions · doi:10.1042/bst20060223 · Cited by 432 · see citing works
THE REGULATION AND PHARMACOLOGY OF ENDOTHELIAL NITRIC OXIDE SYNTHASE
Dudzinski, D. M., Igarashi, J., Greif, D. M., & Michel, T. M. (2005) · The Annual Review of Pharmacology and Toxicology · doi:10.1146/annurev.pharmtox.44.101802.121844 · Cited by 425 · see citing works
Self-citationPurification and characterization of the AMP‐activated protein kinase
Carling, D., Clarke, P. R., Zammit, V. A., & Hardie, D. G. (1989) · European Journal of Biochemistry · doi:10.1111/j.1432-1033.1989.tb15186.x · Cited by 418 · see citing works
Balligand, J., Kobzik, L., Han, X., Kaye, D. M., Belhassen, L., O’Hara, D. S., Kelly, R. A., Smith, T. W., & Michel, T. M. (1995) · Journal of Biological Chemistry · doi:10.1074/jbc.270.24.14582 · Cited by 388 · see citing works
Self-citationMorrow, V. A., Foufelle, F., Connell, J., Petrie, J., Gould, G., & Salt, I. (2003) · Journal of Biological Chemistry · doi:10.1074/jbc.m212831200 · Cited by 384 · see citing works
The role of AMP‐activated protein kinase in mitochondrial biogenesis
Reznick, R. M., & Shulman, G. I. (2006) · The Journal of Physiology · doi:10.1113/jphysiol.2006.109512 · Cited by 371 · see citing works
Sphingosine 1-Phosphate Stimulates Cell Migration through a Gi-coupled Cell Surface Receptor
Wang, F., Brocklyn, J. R. V., Hobson, J. P., Movafagh, S., Żukowska-Grójec, Z., Milstien, S., & Spiegel, S. (1999) · Journal of Biological Chemistry · doi:10.1074/jbc.274.50.35343 · Cited by 367 · see citing works
Mitochondria as signaling organelles in the vascular endothelium
Quintero, M., Colombo, S. L., Godfrey, A. R., & Moncada, S. (2006) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0601026103 · Cited by 358 · see citing works
Horman, S., Vertommen, D., Heath, R. B., Neumann, D., Mouton, V., Woods, A., Schlattner, U., Wallimann, T., Carling, D., Hue, L., et al. (2005) · Journal of Biological Chemistry · doi:10.1074/jbc.m506850200 · Cited by 345 · see citing works
Nagata, D., Mogi, M., & Walsh, K. (2003) · Journal of Biological Chemistry · doi:10.1074/jbc.m300643200 · Cited by 331 · see citing works
Akt1 in Endothelial Cell and Angiogenesis
Somanath, P. R., Razorenova, O. V., Chen, J., & Byzova, T. V. (2006) · Cell Cycle · doi:10.4161/cc.5.5.2538 · Cited by 298 · see citing works
STO-609, a Specific Inhibitor of the Ca2+/Calmodulin-dependent Protein Kinase Kinase
Tokumitsu, H., Inuzuka, H., Ishikawa, Y., Ikeda, M., Saji, I., & Kobayashi, R. (2002) · Journal of Biological Chemistry · doi:10.1074/jbc.m201075200 · Cited by 293 · see citing works
Li, L., Ren, C. H., Tahir, S. A., Ren, C., & Thompson, T. C. (2003) · Molecular and Cellular Biology · doi:10.1128/mcb.23.24.9389-9404.2003 · Cited by 291 · see citing works
Lee, M.-J., Thangada, S., Paik, J.-H., Sapkota, G. P., Ancellin, N., Chae, S.-S., Wu, M.-T., Morales‐Ruiz, M., Sessa, W. C., Alessi, D. R., et al. (2001) · Molecular Cell · doi:10.1016/s1097-2765(01)00324-0 · Cited by 288 · see citing works
Butt, E., Bernhardt, M., Smolenski, A., Kotsonis, P., Fröhlich, L. G., Sickmann, A., Meyer, H. E., Lohmann, S. M., & Schmidt, H. H. H. W. (2000) · Journal of Biological Chemistry · doi:10.1074/jbc.275.7.5179 · Cited by 284 · see citing works
Signaling via Vascular Endothelial Growth Factor Receptors
Petrova, T. V., Mäkinen, T., & Alitalo, K. K. (1999) · Experimental Cell Research · doi:10.1006/excr.1999.4707 · Cited by 275 · see citing works
Statins Activate AMP-Activated Protein Kinase In Vitro and In Vivo
Sun, W., Lee, T., Zhu, M., Gu, C., Wang, Y., Zhu, Y., & Shyy, J. Y.-J. (2006) · Circulation · doi:10.1161/circulationaha.106.630194 · Cited by 264 · see citing works
Sphingosine 1-Phosphate and Activation of Endothelial Nitric-oxide Synthase
Igarashi, J., Bernier, S. G., & Michel, T. M. (2001) · Journal of Biological Chemistry · doi:10.1074/jbc.m008375200 · Cited by 250 · see citing works
Self-citationIgarashi, J., Erwin, P. A., Dantas, A. P., Chen, H., & Michel, T. M. (2003) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1934494100 · Cited by 192 · see citing works
Self-citationTanimoto, T., Jin, Z., & Berk, B. C. (2002) · Journal of Biological Chemistry · doi:10.1074/jbc.m204764200 · Cited by 189 · see citing works
Sambandam, N., & Lopaschuk, G. D. (2003) · Progress in Lipid Research · doi:10.1016/s0163-7827(02)00065-6 · Cited by 171 · see citing works
Carboxyl-terminal isoprenylation of ras-related GTP-binding proteins encoded by rac1, rac2, and ralA
Kinsella, B. T., Erdman, R., & Maltese, W. A. (1991) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)92889-9 · Cited by 160 · see citing works
Qiao, M., Shapiro, P. S., Kumar, R., & Passaniti, A. (2004) · Journal of Biological Chemistry · doi:10.1074/jbc.m404480200 · Cited by 147 · see citing works
Gonzalez, E., Nagiel, A., Lin, A. J., Golan, D. E., & Michel, T. M. (2004) · Journal of Biological Chemistry · doi:10.1074/jbc.m407051200 · Cited by 138 · see citing works
Self-citationInvolvement of Endothelial Nitric Oxide in Sphingosine-1-Phosphate–Induced Angiogenesis
Rikitake, Y., Hirata, K., Kawashima, S., Ozaki, M., Takahashi, T., Ogawa, W., Inoue, N., & Yokoyama, M. (2002) · Arteriosclerosis Thrombosis and Vascular Biology · doi:10.1161/hq0102.101843 · Cited by 122 · see citing works
Schulz, E., Anter, E., Zou, M., & Keaney, J. F. (2005) · Circulation · doi:10.1161/circulationaha.105.546812 · Cited by 114 · see citing works
Greif, D. M., Kou, R., & Michel, T. M. (2002) · Biochemistry · doi:10.1021/bi026732g · Cited by 104 · see citing works
Self-citationGonzalez, E., Kou, R., & Michel, T. M. (2005) · Journal of Biological Chemistry · doi:10.1074/jbc.m510434200 · Cited by 95 · see citing works
Self-citationAMP-activated protein kinase mediates VEGF-stimulated endothelial NO production
Reihill, J. A., Ewart, M., Hardie, D. G., & Salt, I. P. (2007) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2007.01.110 · Cited by 91 · see citing works
Multiple Activation Mechanisms of p38α Mitogen-activated Protein Kinase
Kang, Y. J., Seit‐Nebi, A. S., Davis, R. J., & Han, J. (2006) · Journal of Biological Chemistry · doi:10.1074/jbc.m606800200 · Cited by 91 · see citing works
Thors, B., Halldórsson, H., & Þorgeirsson, G. (2004) · FEBS Letters · doi:10.1016/j.febslet.2004.07.078 · Cited by 84 · see citing works
Activating AMP-Activated Protein Kinase without AMP
Birnbaum, M. J. (2005) · Molecular Cell · doi:10.1016/j.molcel.2005.07.012 · Cited by 81 · see citing works
Role of NADPH Oxidases in the Control of Vascular Gene Expression
Brandes, R. (2003) · Antioxidants and Redox Signaling · doi:10.1089/152308603770380115 · Cited by 71 · see citing works
The Role of Basement Membrane in Angiogenesis and Tumor Growth
Grant, D. S., Kibbey, M. C., Kinsella, J. L., et al. (1994) · Pathology - Research and Practice · doi:10.1016/s0344-0338(11)80989-1 · Cited by 66 · see citing works
Tokumitsu, H., Inuzuka, H., Ishikawa, Y., & Kobayashi, R. (2003) · Journal of Biological Chemistry · doi:10.1074/jbc.m213183200 · Cited by 51 · see citing works
Zhang, Q., Adiseshaiah, P. P., Kalvakolanu, D. V., & Reddy, S. P. (2006) · Journal of Biological Chemistry · doi:10.1074/jbc.m513008200 · Cited by 43 · see citing works
Kou, R., SenBanerjee, S., Jain, M. K., et al. (2005) · Biochemistry · doi:10.1021/bi0509898 · Cited by 32 · see citing works
Involvement of Nitric Oxide in Angiogenic Activities of Vascular Endothelial Growth Factor Isoforms
Józkowicz, A., Dulak, J., Nigisch, A., Funovics, P. T., Weigel, G., Polterauer, P., Huk, I., & Maliñski, T. (2004) · Growth Factors · doi:10.1080/0897719041682863 · Cited by 32 · see citing works
Seebach, J., Mädler, H., Wójciak‐Stothard, B., et al. (2005) · Thrombosis and Haemostasis · doi:10.1160/th05-01-0015 · Cited by 31 · see citing works
Palanivel, R., Veluthakal, R., McDonald, P., et al. (2005) · Endocrine · doi:10.1385/endo:26:1:071 · Cited by 11 · see citing works
Flagged first, then by citations
Research works printed but not linked: 4
- Shaw, R. J., Kosmatka, M., Bardeesy, N., Hurley, R. L., Witters, L. A., DePinho, R. A., and Cantley, L. C. (2004) Proc. Natl. Acad. Sci. U. S. A. 101, 3329 -3335
- Quintero, M., Colombo, S. L., Godfrey, A., and Moncada, S. (2006) Proc. Natl. Acad. Sci. U. S. A. 103, 5379 -5384
- Igarashi, J., Erwin, P. A., Dantas, A. P., Chen, H., and Michel, T. (2003) Proc. Natl. Acad. Sci. U. S. A. 100, 10664 -10669
- Brownsey, R. W., Boone, A. N., Elliott, J. E., Kulpa, J. E., and Lee, W. M. (2006) Biochem. Soc. Trans. 34, 223-227
Cited by
Sorted by citations
Receptor-mediated activation of ceramidase activity initiates the pleiotropic actions of adiponectin
Holland, W. L., Miller, R. A., Wang, Z. V., Sun, K., Barth, B. M., Bui, H. H., Davis, K. E., Bikman, B. T., Halberg, N., Rutkowski, J. M., et al. (2011) · Nature Medicine · doi:10.1038/nm.2277 · Cited by 892 · see citing works
Cellular and molecular biology of aging endothelial cells
Donato, A. J., Morgan, R. G., Walker, A. E., & Lesniewski, L. A. (2015) · Journal of Molecular and Cellular Cardiology · doi:10.1016/j.yjmcc.2015.01.021 · Cited by 538 · see citing works
Balligand, J.-L., Feron, O., & Dessy, C. (2009) · Physiological Reviews · doi:10.1152/physrev.00042.2007 · Cited by 477 · see citing works
Rivera, L., Morón, R., Zarzuelo, A., & Galisteo, M. (2009) · Biochemical Pharmacology · doi:10.1016/j.bcp.2008.11.027 · Cited by 391 · see citing works
Activation and Signaling by the AMP-Activated Protein Kinase in Endothelial Cells
Fisslthaler, B., & Fleming, I. (2009) · Circulation Research · doi:10.1161/circresaha.109.201590 · Cited by 295 · see citing works
AMPK, Mitochondrial Function, and Cardiovascular Disease
Wu, S., & Zou, M.-H. (2020) · International Journal of Molecular Sciences · doi:10.3390/ijms21144987 · Cited by 286 · see citing works
AMP-activated protein kinase functionally phosphorylates endothelial nitric oxide synthase Ser633
Chen, Z., Peng, I.-C., Sun, W., Su, M.-I., Hsu, P.-H., Fu, Y., Zhu, Y., DeFea, K., Pan, S., Tsai, M.-D., et al. (2009) · Circulation Research · doi:10.1161/circresaha.108.187567 · Cited by 264 · see citing works
Diversity and plasticity of microglial cells in psychiatric and neurological disorders
Nakagawa, Y., & Chiba, K. (2015) · Pharmacology & Therapeutics · doi:10.1016/j.pharmthera.2015.06.010 · Cited by 208 · see citing works
Koska, J., Sands, M., Burciu, C., D’Souza, K. M., Raravikar, K., Liu, J., Truran, S., Franco, D. A., Schwartz, E. A., Schwenke, D. C., et al. (2015) · Diabetes · doi:10.2337/db14-0976 · Cited by 174 · see citing works
Zhang, Y., Recouvreux, M. V., Jung, M., Galenkamp, K. M. O., Li, Y., Zagnitko, O., Scott, D. A., Lowy, A. M., & Commisso, C. (2021) · Cancer Discovery · doi:10.1158/2159-8290.cd-20-0119 · Cited by 116 · see citing works
Xing, S.-S., Yang, X.-Y., Zheng, T., Li, W.-J., Wu, D., Chi, J.-Y., Bian, F., Bai, X.-L., Wu, G.-J., Zhang, Y.-Z., et al. (2015) · Vascular Pharmacology · doi:10.1016/j.vph.2015.07.004 · Cited by 99 · see citing works
Vázquez-Chantada, M., Ariz, U., Varela-Rey, M., Embade, N., Martínez-Lopez, N., Fernández-Ramos, D., Gómez-Santos, L., Lamas, S., Lu, S. C., Martínez-Chantar, L. M., et al. (2009) · Hepatology · doi:10.1002/hep.22660 · Cited by 76 · see citing works
Ou, H.-C., Hsieh, Y.-L., Yang, N.-C., Tsai, K.-L., Chen, K.-L., Tsai, C.-S., Chen, I.-J., Wu, B.-T., & Lee, S.-D. (2013) · Journal of Applied Physiology · doi:10.1152/japplphysiol.00367.2012 · Cited by 34 · see citing works
- Authors
- Levine, Y. C., Li, G. K., & Michel, T.
- Affiliations
- Brigham and Women's Hospital (US)
- Corresponding author
- Michel, Thomas
- ORCID
- Michel, Thomas
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 2007-07
- Volume
- 282
- Issue
- 28
- Pages
- 20351-20364
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W2091263181
- Cited by
- 207
- References
- 62
- Citations by year
- 2026: 2 · 2025: 2 · 2024: 5 · 2023: 7 · 2022: 8 · 2021: 9 · 2020: 8 · 2019: 8 · 2018: 6 · 2017: 10 · 2016: 7 · 2015: 14 · 2014: 11 · 2013: 15 · 2012: 21
- FWCI
- 12.03
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Journal of Biological Chemistry (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Funders
- Sarnoff Cardiovascular Research Foundation
- National Institutes of Health (HL48743, HL46457, GM36259)
- National Heart, Lung, and Blood Institute (HL 46457, HL-48743, P01HL048743)
- National Institute of General Medical Sciences (GM 36259)
- Topics
- Health Sciences › Medicine › Physiology › Nitric Oxide and Endothelin Effects
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Metabolism, Diabetes, and Cancer
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Kinase Regulation and GTPase Signaling
- Keywords
- AMPK, endothelial nitric oxide synthase, endothelial cells, vascular endothelial growth factor, sphingosine-1-phosphate, AMPKα1, AKT1, Rac1, caveolin-1, phosphatidylinositol 3-kinase, vascular homeostasis, signal transduction
- MeSH terms
- Wortmannin, Androstadienes, Animals, Cattle, Cells, Cultured, Enzyme Activation, Homeostasis, Lysophospholipids, Multienzyme Complexes, Phosphorylation, Sphingosine, Protein Serine-Threonine Kinases, Calcium-Calmodulin-Dependent Protein Kinases, Phosphatidylinositol 3-Kinases, Calcium Signaling, rac1 GTP-Binding Protein, RNA, Small Interfering, Vascular Endothelial Growth Factor A, Endothelial Cells, Protein Kinase Inhibitors, Proto-Oncogene Proteins c-akt, Caveolin 1, Nitric Oxide Synthase Type III, AMP-Activated Protein Kinases
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
