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A Requirement for Lipids by the Microsomal Stearyl Coenzyme A Desaturase
Open accessAbstract
Abstract Hen liver microsomes, when extracted with aqueous acetone, lose their ability to desaturate stearyl coenzyme A to oleate. The desaturase activity of these particles is partially restored by addition of lipid micelles prepared from microsomal lipid. Fractionation of the microsomal lipid yields fractions which are tested for their ability to restore the desaturase activity. A mixture of phospholipids, triglycerides, and fatty acids is able to restore the desaturase activity of the acetone-extracted microsomes to the level of the original microsomes. Phosphatidylcholine, isolated from the phospholipid, can partially replace phospholipids in this mixture. The properties of the reconstituted system are similar to those of the original microsomes. There is an absolute requirement for oxygen and a reduced pyridine nucleotide, although DPNH, rather than TPNH, is the preferred electron donor. The similarities between the lipid requirements of the DPNH-cytochrome c reductase and the stearyl-CoA desaturase support the suggestion that the two systems share a common electron transfer pathway or carrier.
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Cited by
Lu, A. Y. H., Junk, K. W., & Coon, M. J. (1969) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)83427-5 · Cited by 577
Purification and Properties of Rat Liver Microsomal Stearyl Coenzyme A Desaturase
Strittmatter, P., Spatz, L., Corcoran, D., Rogers, M. J., Setlow, B., & Redline, R. (1974) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.71.11.4565 · Cited by 469
The oxidative desaturation of unsaturated fatty acids in animals
Brenner, R. R. (1974) · Molecular and Cellular Biochemistry · doi:10.1007/bf01660076 · Cited by 391
The gradient hypothesis and other models of carrier-mediated active transport
Crane, R. K. (2005) · Reviews of Physiology, Biochemistry and Pharmacology · doi:10.1007/bfb0027722 · Cited by 324
Role of stearoyl-coenzyme A desaturase in lipid metabolism
Miyazaki, M., & Ntambi, J. M. (2003) · Prostaglandins, Leukotrienes and Essential Fatty Acids · doi:10.1016/s0952-3278(02)00261-2 · Cited by 295
Metadata
- Authors
- Jones, P. D., Holloway, P. W., Peluffo, R. O., & Wakil, S. J.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1969-02
- Volume
- 244
- Issue
- 3
- Pages
- 744-754
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W1570602387
- Cited by
- 158
- References
- 0
- FWCI
- 8.05
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Jones, P. D., Holloway, P. W., Peluffo, R. O., & Wakil, S. J. (1969). A Requirement for Lipids by the Microsomal Stearyl Coenzyme A Desaturase. Journal of Biological Chemistry. https://doi.org/10.1016/s0021-9258(18)94415-7
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