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The Release of Enzymes by Osmotic Shock from Escherichia coli in Exponential Phase
Open accessAbstract
A number of degradative enzymes are specifically released from exponentially growing Escherichia coli by osmotic shock.They include alkaline phosphatase, cyclic phosphodiesterase, 5'-nucleotidase, acid phosphatase, and the ribonucleic acidinhibited endonuclease that is active with deoxyribonucleic acid.Small amounts of RNase are also set free, but some 16 control enzymes are found to remain within the cell.Only about 4% of the cellular protein is released into the shock medium.The procedure for osmotic shock has been modified in order to obtain about 80% viability with exponential cells, with good release of enzymes.In the first stage, cells are exposed to 20% sucrose-1 X 10 -4 M EDTA.In the second stage, the pellet of sucrose-treated cells is rapidly dispersed in cold 5 X 10 -4 M MgC 1 2, which causes the enzymes to be liberated.At least 90% of the DNA endonuclease and 50% of the latent ribonuclease are released from exponentially growing E. coli when as much as 2 X 10 -2 M MgC1 2 is used in the procedure for osmotic shock.
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Cited by
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Barker, D. J., & Stuckey, D. C. (1999) · Water Research · doi:10.1016/s0043-1354(99)00022-6 · Cited by 905
The purification of eukaryotic polypeptides synthesized in Escherichia coli
Marston, F. A. (1986) · Biochemical Journal · doi:10.1042/bj2400001 · Cited by 836
Lugtenberg, B., & Van Alphen, L. (1983) · Biochimica et Biophysica Acta (BBA) - Reviews on Biomembranes · doi:10.1016/0304-4157(83)90014-x · Cited by 759
Grodberg, J., & Dunn, J. J. (1988) · Journal of Bacteriology · doi:10.1128/jb.170.3.1245-1253.1988 · Cited by 676
Mutant phenotypes for thousands of bacterial genes of unknown function
Price, M. N., Wetmore, K. M., Waters, R. J., Callaghan, M., Ray, J., Liu, H., Kuehl, J. V., Melnyk, R. A., Lamson, J. S., Suh, Y., et al. (2018) · Nature · doi:10.1038/s41586-018-0124-0 · Cited by 576
Metadata
- Authors
- Nossal, N. G., & Heppel, L. A.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1966-07
- Volume
- 241
- Issue
- 13
- Pages
- 3055-3062
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W1612160157
- Cited by
- 670
- References
- 0
- FWCI
- 20.55
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Nossal, N. G., & Heppel, L. A. (1966). The Release of Enzymes by Osmotic Shock from Escherichia coli in Exponential Phase. Journal of Biological Chemistry. https://doi.org/10.1016/s0021-9258(18)96497-5
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